Effects of temperature and salinity interaction on Vibrio spp and Vibrio parahaemolyticus in the intercontinental Euro-African Atlantic

Abstract

Vibrionaceae include several human pathogens such as Vibrio cholerae, Vibrio vulnificus or Vibrio parahaemolyticus. The risk of vibriosis is increasing worldwide due to the effects of climate change and modified patterns of food consumption, leading to a considerable economic and public health interest in understanding the factors related to the greater abundance of vibrios. Fluctuations in Vibrio populations are strongly affected by changes in temperature and salinity, nonetheless, there is substantial variability in their effect and discrepancies in their specific roles. In this study, we analyzed the abundance, and spatiotemporal distribution of Vibrio in the Euro-African Atlantic area, focusing the investigation on associations with environmental factors and with an emphasis on V. parahaemolyticus. Using membrane-filtration, cultures and MALDI-TOF mass spectrometry, 191 samples from 12 locations were analysed and a biochemical and proteomic profile created. We developed two multivariate linear regression models for the density of Vibrio spp (adjusted R2 = 0.32 and 0.27) and a logistic regression model for the occurrence of V. parahaemolyticus (R2Nagelkerke = 0.32, Accuracy = 77%). Including the interaction between sea surface temperature (SST) and salinity in linear regression helps to explain the discrepancies found in several studies on the effect of these variables on the abundance of vibrios.

16S Next-generation sequencing and quantitative PCR reveal the distribution of potential pathogens in the Liaohe Estuary

“…Recent studies have shown that few environmental pathogens, such as Legionella pneumophila and Pseudomonas aeruginosa, were responsible for increasing outbreaks (Percival, 2014;Liang et al, 2020). Some enteric pathogens, such as Vibrio, Escherichia coli, and Salmonella spp., are attributed to an increase in seafood disease outbreaks (Ramaiah, 2002;Rodrigues et al, 2011;Khandeparker et al, 2015), particularly Vibrio parahaemolyticus and Vibrio vulnificus (Ruiz-Cayuso et al, 2021). Vibrio-infected oysters caused the first major outbreak in Europe in 1999 (Lozano-Leoń et al, 2003).…”