Effects of temperature and salinity on juveniles of two ascidians, one native and one invasive, and their hybrids

Malfant, Marine; Coudret, Jérôme; Merdy, Régis Le; Viard, Frédérique

doi:10.1016/j.jembe.2017.09.019

Cited by 15 publications

(30 citation statements)

References 38 publications

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“…environmental barriers) do not seem neither to be effective against gene flow between the two species in their sympatry area. Hybrids are indeed performing as well as their parental species under various temperature and salinity conditions 53 . It is thus likely that intrinsic barriers, notably due to genomic incompatibilities in second generation hybrids (i.e.…”

Section: Discussionmentioning

confidence: 97%

“…Experiments were made following protocols provided in Malfant, et al . 53 . Briefly, the number of oocytes of one individual was estimated in three drops of 150 µl.…”

Section: Methodsmentioning

confidence: 99%

“…intestinalis as maternal lineage Malfant et al . 53 “Original name” corresponds to the species name used in the paper whereas “Putative name” is the species name that we propose to be the most likely following the taxonomic revision and nomenclature accepted from September 2015. …”

Section: Introductionmentioning

confidence: 99%

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Coupling molecular data and experimental crosses sheds light about species delineation: a case study with the genus Ciona

Malfant

Darras

Viard

2018

Sci Rep

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Molecular studies sometimes reveal evolutionary divergence within accepted species. Such findings can initiate taxonomic revision, as exemplified in the formerly recognized species Ciona intestinalis. While an increasing number of studies have examined the ecology, reproductive barriers and genetics of C. intestinalis and C. robusta, there are still much uncertainties regarding other species of this genus. Using experimental crosses and mitochondrial data, we investigated the evolutionary relationships among four native and introduced Ciona spp., found in sympatry in the Mediterranean Sea or English Channel. Outcome of 62 bi-parental reciprocal crosses between C. intestinalis, C. robusta, C. roulei and C. edwardsi showed that C. edwardsi is reproductively isolated from the other taxa, which is in agreement with its distinct location in the phylogenetic tree. Conversely, hybrids are easily obtained in both direction when crossing C. intestinalis and C. roulei, reinforcing the hypothesis of two genetically differentiated lineages but likely being from a same species. Altogether, this study sheds light on the evolutionary relationship in this complex genus. It also calls for further investigation notably based on genome-wide investigation to better describe the evolutionary history within the genus Ciona, a challenging task in a changing world where biological introductions are shuffling species distribution.

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Section: Discussionmentioning

confidence: 97%

“…Experiments were made following protocols provided in Malfant, et al . 53 . Briefly, the number of oocytes of one individual was estimated in three drops of 150 µl.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Coupling molecular data and experimental crosses sheds light about species delineation: a case study with the genus Ciona

Malfant

Darras

Viard

2018

Sci Rep

Self Cite

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“…Mechanisms ensuring species-specific fertilization are important for prezygotic reproductive isolation (Mayr 1963;Seehausen et al 2014;Herberg et al 2018) , but successful fertilization between C. robusta and C. intestinalis can routinely be obtained in the laboratory, despite indications that C. intestinalis sperm occasionally fails to fertilize C. robusta eggs (Suzuki et al 2005;Sato et al 2014;Bouchemousse et al 2016a;Malfant et al 2017) . Notably, Ciona adults are self-incompatible hermaphrodites (Harada et al 2008;Sawada et al 2020) , which spawn their gametes in the open water at dawn.…”

Section: Introductionmentioning

confidence: 99%

“…Sperm and eggs were surgically obtained from mature animals, and were used for controlled in vitro fertilizations to produce F1 generation animals, using established protocols (Christiaen et al 2009) . We cultured all animals at 18°C (Sanyo, MIR-154), a permissive temperature for both species, as well as for F1 hybrids (Sato et al 2014;Malfant et al 2017) . Juveniles were kept in Petri dishes at 18°C until 28 days post fertilization (dpf).…”

Section: Introductionmentioning

confidence: 99%

Asymmetric second-generation genomic incompatibility in interspecific crosses betweenCiona robustaandCiona intestinalis

Ohta

Kaplan

et al. 2020

Preprint

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Reproductive isolation is central to speciation, but interspecific crosses between two closely related species can produce viable and fertile hybrids. Two different species in the tunicate genus Ciona , Ciona robusta and Ciona intestinalis can produce hybrids. However, wild sympatric populations display limited gene flow, suggesting the existence of obstacles to interspecific reproduction that remain unknown. Here, we took advantage of a closed inland culture system to cross C. robusta with C. intestinalis and established F1 and F2 hybrids. We monitored post-embryonic development, survival, and sexual maturation to further probe the physiological mechanisms underlying reproductive isolation. Partial viability of first and second generation hybrids indicated that both pre-and postzygotic mechanisms contributed to genomic incompatibilities in hybrids. Asymmetrical second generation inviability and infertility suggested that interspecific genomic incompatibilities involved interactions between the maternal, zygotic and mitochondrial genomes during development. This study paves the way to quantitative genetic approaches to study the mechanisms underlying genomic incompatibilities and other complex traits in the genome-enabled Ciona model.

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Effects of Temperature and Salinity on the Egg Development and Larval Settlement of Ciona robusta (Ascidiacea, Phlebobranchia, Cionidae)

Kim

Park

et al. 2018

Ocean Sci. J.

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Effects of temperature and salinity on juveniles of two ascidians, one native and one invasive, and their hybrids

Cited by 15 publications

References 38 publications

Coupling molecular data and experimental crosses sheds light about species delineation: a case study with the genus Ciona

Coupling molecular data and experimental crosses sheds light about species delineation: a case study with the genus Ciona

Asymmetric second-generation genomic incompatibility in interspecific crosses betweenCiona robustaandCiona intestinalis

Effects of Temperature and Salinity on the Egg Development and Larval Settlement of Ciona robusta (Ascidiacea, Phlebobranchia, Cionidae)

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