Effects of Temperature and Light on the Carbon Budget of Young Cucumber Plants Studied by Steady-State Feeding with<sup>14</sup>CO<sub>2</sub>

Shishido, Yoshihiro; Challa, H.; Krupa, J.

doi:10.1093/jxb/38.6.1044

Cited by 27 publications

(24 citation statements)

References 5 publications

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“…3, D-F, and 4A). Many calculations of export have also been obtained by first labeling the leaf and then analyzing the amount of radioactivity remaining in the leaf following a chase period of minutes, hours, and in some cases, even days (see Webb, 1970;Shishido et al, 1987;Farrar, 1988;Moing et al, 1992, and refs. therein).…”

Section: Varying Leaf Temperaturementioning

confidence: 99%

The Effect of Leaf Temperature and Photorespiratory Conditions on Export of Sugars during Steady-State Photosynthesis in Salvia splendens

Jiao

Grodzinski

1996

Plant Physiology

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Export and photosynthesis i n leaves of Salvia splendens were measured concurrently during steady-state 14C0, labeling conditions. Under ambient CO, and O, conditions, photosynthesis and export rates were similar at 15 and 25"C, but both declined as leaf temperature was raised from 25 to 40°C. Suppressing photorespiration between 15 and 40°C by manipulating CO, and O, levels resulted in higher rates of leaf photosynthesis, total sugar synthesis, and export. There was a linear relationship between the rate of photosynthesis and the rate of export between 15 and 35°C. At these temperatures, 60 to 80% of the carbon fixed was readily exported with sucrose, raffinose, and stachyose, which together constituted over 90% of phloem mobile assimilates. Above 35"C, however, export during photosynthesis was inhibited both in photorespiratory conditions, which inhibited photosynthesis, and in nonphotorespiratory conditions, which did not inhibit photosynthesis. Sucrose and raffinose but not stachyose accumulated i n the leaf at 40°C. When leaves were preincubated at 40°C and then cooled to 3 5 T , export recovered more slowly than photosynthesis. These data are consistent with the view that impairment of export processes, rather than photosynthetic processes associated with light trapping, carbon reduction, and sucrose synthesis, accounted for the marked reduction i n export between 35 and 40°C. Taken together, the data indicated that temperature changes between 15 and 40°C had two effects on photosynthesis and concurrent export. At all temperatures, suppressing photorespiration increased both photosynthesis and export, but above 35"C, export processes were more directly inhibited independent of changes i n photorespiration and photosynthesis.

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Section: Varying Leaf Temperaturementioning

confidence: 99%

The Effect of Leaf Temperature and Photorespiratory Conditions on Export of Sugars during Steady-State Photosynthesis in Salvia splendens

Jiao

Grodzinski

1996

Plant Physiology

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“…Shishido et al (1988) investigated the relationship between vascular architecture and photosynthate translocation in tomato by using 14 C. Furthermore, the carbon balance of a single leaf can be obtained by 14 CO 2 steady-state feeding methods, which enable the rates of photosynthesis, translocation, and respiration to be quantified (Shishido et al, 1987(Shishido et al, , 1989. The stable isotope 13 C has been used similarly (Bledsoe and Orians, 2006).…”

Section: Introductionmentioning

confidence: 99%

Real-time Analysis of Photoassimilate Translocation in Intact Eggplant Fruit using 11CO2 and a Positron-emitting Tracer Imaging System

Kikuchi

Ishii

Fujimaki

et al. 2008

J. Japan. Soc. Hort. Sci.

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A recently developed positron-emitting tracer imaging system (PETIS) noninvasively produces quantitative realtime images of the movement of various compounds in plants. To clarify the mechanism of the growth and development of the fruit of eggplant (Solanum melongena L.), we fed 11 CO 2 to a leaf and monitored the translocation of 11 C-labeled photoassimilate into the fruit by PETIS. Continuous images of the translocation of [11 C]photoassimilate from the leaf to the fruit and inside the fruit were produced, with a shorter time resolution than reported previously. 11 C signal intensity in the fruit increased gradually, and its distribution was not uniform. The fruits were sliced after PETIS measurement and exposed to the imaging plates of a bio-imaging analyzer system. The resultant images showed the localization of 11 C activity inside the fruit, and indicated that translocation of photoassimilate within the fruit was not uniform. The velocity of photoassimilate translocation in the peduncle and changes in the rate of translocation of photoassimilate in the fruit were estimated by analysis of PETIS data. The velocity of photoassimilate translocation through the peduncle was estimated as 1.17 cm·min −1. About 60 min after the start of 11 CO 2 feeding, the 11 C activity of the fruit began to increase, and by 120 min it had reached about 8% of feeding 11 CO 2 . These results indicate that it took about 60 min for the first [11 C]photoassimilates to reach the fruit. Real time observation of photoassimilate translocation within a fruit has never been reported. PETIS may be a powerful tool for revealing the mechanisms of fruit development and maturity.

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“…Methods of data analysis need to take into account that carbon available to a given organ generally is a composite of recently fixed carbon supplied by several possible sources including direct fixation, as well as of stored carbon from source, path and sink organs. Whole-plant labelling methods are needed to complement steady-state labelling of a single leaf with COj as the latter characterizes distribution of tracer carbon from that leaf but does not measure the total carbon influx contributed by the different sources (Shishido, Challa & Krupa, 1987),…”

Section: Introductionmentioning

confidence: 99%

Analysing partitioning of recently fixed and of reserve carbon in reproductive Phaseolus vulgaris L. plants

Geiger

Shieh

1988

Plant Cell & Environment

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Abstract. Partitioning of recently‐fixed carbon among plant organs and subsequent distribution of reserve carbon were studied by supplying whole shoots of bean plants (Phaseolus vulgaris L.) with 14C‐labelled CO2 of constant specific radioactivity throughout a photoperiod. The gain of tracer carbon in each part revealed net accumulation of recently‐fixed carbon from direct fixation, import or both. Growth rate coefficients describing the present pattern of plant growth were calculated from ratios of tracer carbon to total carbon present in plant organs and were used to project future plant form. The period 10–20 d after the start of flowering was marked by a major increase in partitioning of recently‐fixed carbon to reproductive growth. Growth rates for the plant and its parts during this period were projected on the basis of growth rate coefficients and were found to be similar to rates measured by gravimetric growth analysis. Changes in tracer carbon recovered in individual organs after chase periods of various lengths revealed net decreases for leaves and stems. About 9% of the carbon distributed to fruits came from reserves even in the absence of obvious stress. Respiratory loss during the chase period was determined from the progressive drop in recovery of the original tracer carbon. The methods are being applied to measure current net accumulation rates in studies of sink organ physiology, and to compare partitioning of recently‐fixed and of stored carbon in several plant species under defined growth conditions.

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Effects of Temperature and Light on the Carbon Budget of Young Cucumber Plants Studied by Steady-State Feeding with¹⁴CO₂

Cited by 27 publications

References 5 publications

The Effect of Leaf Temperature and Photorespiratory Conditions on Export of Sugars during Steady-State Photosynthesis in Salvia splendens

The Effect of Leaf Temperature and Photorespiratory Conditions on Export of Sugars during Steady-State Photosynthesis in Salvia splendens

Real-time Analysis of Photoassimilate Translocation in Intact Eggplant Fruit using 11CO2 and a Positron-emitting Tracer Imaging System

Analysing partitioning of recently fixed and of reserve carbon in reproductive Phaseolus vulgaris L. plants

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Effects of Temperature and Light on the Carbon Budget of Young Cucumber Plants Studied by Steady-State Feeding with14CO2

Cited by 27 publications

References 5 publications

The Effect of Leaf Temperature and Photorespiratory Conditions on Export of Sugars during Steady-State Photosynthesis in Salvia splendens

The Effect of Leaf Temperature and Photorespiratory Conditions on Export of Sugars during Steady-State Photosynthesis in Salvia splendens

Real-time Analysis of Photoassimilate Translocation in Intact Eggplant Fruit using 11CO2 and a Positron-emitting Tracer Imaging System

Analysing partitioning of recently fixed and of reserve carbon in reproductive Phaseolus vulgaris L. plants

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Effects of Temperature and Light on the Carbon Budget of Young Cucumber Plants Studied by Steady-State Feeding with¹⁴CO₂