Effects of Temperature and Culture Media Composition on Sporulation, Mycelial Growth, and Antifungal Activity of Isaria javanica pf185

Abstract

The fungal isolate Isaria javanica pf185 has potential as a mycopesticide because it demonstrates insecticidal activity against the green peach aphid and antifungal activity against Colletotrichum gloeosporioides. For commercialization of this isolate, determination of the optimal and least expensive culture conditions is required; however, these data are not currently available. This study describes the conditions for optimal development of conidia and production of metabolites for the biocontrol of the fungal pathogen. The optimal culture conditions were examined using cultures on solid agar and liquid media. High growth temperature enhanced spore formation but reduced antifungal activity in both solid and liquid media. The highest spore yield was obtained in a medium containing glucose as a carbon source and yeast extract as a nitrogen source. Soybean powder and wheat bran were effective nitrogen sources that promoted spore production and antifungal activity of the isolate. These results revealed the basic, cost-effective growth media for commercial production of a biopesticide with insecticidal and antifungal properties for use in integrated pest management.

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“…After 15 days, the plates were flooded with 5 mL of sterile distilled water and conidia were dislodged using a small brush. The suspension was homogenized by vigorous shaking in a vortex shaker (Vortex-Genie® 2 Shaker, Mumbai, India) for 10 min ( Lee et al, 2021 ). A drop of filtered spore suspension was placed on the hemocytometer (Bright-Line™, Hemacytometer, Darmstadt, Germany) and spores were counted under the microscope (Nikon Eclipse Ci, Japan).…”