Effects of taurine and hypotaurine supplementation and ionophore concentrations on post-thaw acrosome reaction of dog spermatozoa

Martins‐Bessa, Ana; Rocha, António; Mayenco-Aguirre, A.

doi:10.1016/j.theriogenology.2008.07.006

Cited by 22 publications

(14 citation statements)

References 35 publications

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“…3). Anti-oxidants, such as amino acids like taurine, hypotaurine [16,17] and hyaluronic acid [19], may be needed for maintenance of motility and viability at 5 C or room temperature.…”

Section: Discussionmentioning

confidence: 99%

Effects of Semen Extenders and Storage Temperatures on Characteristics of Frozen-Thawed Bryde's (Balaenoptera edeni) Whale Spermatozoa

Hiwasa

Suzuki

Watanabe

et al. 2009

Journal of Reproduction and Development

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Abstract. The present study investigated effects of three semen extenders and storage temperatures on post-thaw characteristics of Bryde's whale spermatozoa. Spermatozoa were collected from the vasa deferens of three mature Bryde's whales captured during the Japanese whale research in the north-west Pacific (May to August 2007) after death. The three semen extenders used for freezing were 1) a commercialized synthetic extender (AndroMed: AM), 2) Trisbased + 10% bovine serum albumin (BSA) and 3) Tris-based + egg yolk (EY). The sperm samples from the three whales were frozen with the three extenders, and the post-thaw spermatozoa were stored at three different temperatures (35 C; 20-25 C, room temperature; and 5 C) for 0, 6, 12, 24, 48 and 96 h. At each time-point, total and progressive motility (PM), viability (live or dead), the hypo-osmotic test, defective acrosomes and malformation were examined. Immediately after thawing, AM resulted in similar recovery rates (60.4 and 83.3%) in 2 of the 3 whales examined and had comparable post-thaw recovery rates to those obtained using the EY and BSA extenders. Immediately after thawing, the proportion of PM in EY (17.6%) was higher (P<0.05) than that in BSA (15.0%). In the hypo-osmotic test, the proportions of AM (26.0%) and BSA (25.2%) were higher (P<0.05) than that of EY (17.3 %). The three extenders had similar viabilities (36.7, 37.9 and 32.1%, respectively), but the viability of BSA was higher (P<0.05) than that of EY. The present study showed that a synthetic semen extender, AndroMed, could be used for cryopreservation of whale spermatozoa in addition to Tris-based extenders containing bovine serum albumin or egg yolk. Storage of the post-thaw Bryde's whale spermatozoa was better at 5 C than at room temperature or 35 C. The frozen-thawed Bryde's whale spermatozoa maintained their motility and viability for at least two days at room temperature and for four days at 5 C. [5] used Bryde's (Balaenoptera edeni) whale spermatozoa freshly-diluted with m-PBS and stored at 4 C for in vitro fertillization (IVF) in the field. However, it has not been demonstrated yet how long the viability of frozen-thawed spermatozoa is maintained and what the most appropriate temperature is for post-thaw storage in baleen whales, including Bryde's whales. For domestic animals including sheep, post-thaw semen is usually maintained at 37 C for examinations of their motility and viability, but the body temperatures (35.5 to 37 C) of dolphins and whales are lower than those of other mammals, and no one has measured the body temperatures of live baleen whales, including Antarctic minke and Bryde's whales.There has been recent movement to eliminate all animal ingredients, such as egg yolk, milk and even bovine serum albumin (BSA), from semen extenders. Our recent studies [6-8] on artificial insemination (AI) in sheep have clearly shown that a soybean-based semen extender (AndroMed) is useful and comparable to the conventional semen extenders containing egg yolk or BSA. Fukui et al. [6] compared ...

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“…3). Anti-oxidants, such as amino acids like taurine, hypotaurine [16,17] and hyaluronic acid [19], may be needed for maintenance of motility and viability at 5 C or room temperature.…”

Section: Discussionmentioning

confidence: 99%

Effects of Semen Extenders and Storage Temperatures on Characteristics of Frozen-Thawed Bryde's (Balaenoptera edeni) Whale Spermatozoa

Hiwasa

Suzuki

Watanabe

et al. 2009

Journal of Reproduction and Development

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“…Tris-based egg yolk extenders are frequently used for semen cryopreservation in domestic animals including buffaloes (Barbas and Mascarenhas 2008). In recent years, cryoprotectants like taurine, trehalose, 4-bromophenacyl bromide and catalase were supplemented to freezing extender of bull (Chen et al 1993;Uysal et al 2007;Sariozkan et al 2009), boar (Funahashi and Sano 2005;Gutierrez-Perez et al 2009;Hu et al 2009), ram Bucak and Uysal 2008), goat (Atessahin et al 2008), dog (Michael et al 2007;Martins-Bessa et al 2009) and human (Tiziana et al 2001) semen to improve the sperm characteristics after cryopreservation. The sulphonic amino acid, taurine, acts as an antioxidant and can traverse the sperm plasma membrane, inhibit lipid peroxidation, protect the cells against accumulation of reactive oxygen species (Chen et al 1993;Foote et al 2002), modulate Ca 2+ uptake and inhibit protein phosphorylation (Lombardini 1985).…”

Section: Introductionmentioning

confidence: 99%

Effect of Incorporation of Additives in Tris‐Based Egg Yolk Extender on Buffalo (Bubalus bubalis) Sperm Tyrosine Phosphorylation During Cryopreservation

Kumar

Atreja

2011

Reprod Domestic Animals

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Phosphorylation of tyrosine residues on sperm protein is a known indicator of capacitation and a major intracellular signalling event. There is evidence that sperm cryopreservation promotes tyrosine phosphorylation and is associated with reduced fertility of spermatozoa. Under this study, cryoprotective role of different additives namely taurine, trehalose, catalase and 4-bromophenacyl bromide on buffalo sperm quality was evaluated. Buffalo semen was cryopreserved in tris-based egg yolk extender supplemented with additives like taurine (50 mm) or trehalose (100 mm) or 4-bromophenacyl bromide (200 μm) or catalase (100 U/ml) and used for assessment of levels of tyrosine phosphorylation in frozen-thawed spermatozoa. The results obtained were compared with the level of protein tyrosine phosphorylation of semen cryopreserved in tris-based egg yolk extender without additives. Proteins were extracted from a total number of nine ejaculates from three individual buffalo bulls chosen at random and analysed for tyrosine phospho-proteins using SDS-PAGE followed by immunoblotting. Monoclonal anti-phosphotyrosine antibody (Clone pT-154) was used as primary antibody followed by treatment with HRP-conjugated secondary antibody. Signals were detected on X-ray film using chemiluminescence. Nine proteins (p20, p30, p32, p38, p49, p56, p59, p72 and p86) were found to be tyrosine phosphorylated in cryopreserved spermatozoa. Supplementation of additives significantly (p<0.05) reduced the level of protein tyrosine phosphorylation in spermatozoa. Moreover, this study showed improved (p<0.05) post-thaw motility, viability and membrane integrity of spermatozoa on addition of these additives. The results obtained clearly indicate reduced level of capacitation like changes on supplementation of additives in terms of protein tyrosine phosphorylation.

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“…2007), goat (Atessahin et al. 2008) and dog spermatozoa (Martins‐Bessa et al. 2009) which showed improved semen characteristics upon cryopreservation.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Intracellular Ca²⁺, cAMP and 1,2‐Diacylglycerol in Cryopreserved Buffalo (Bubalus bubalis) Spermatozoa on Supplementation of Taurine and Trehalose in the Extender

Singh

Atreja

Kumar

et al. 2011

Reprod Domestic Animals

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In mammalian spermatozoa, intracellular calcium plays a major role in sperm functions like motility and capacitation. Cryopreservation-induced modifications to sperm membrane result in an influx of intracellular calcium affecting calcium-dependent intracellular signalling pathways. Intracellular calcium activates adenyl cyclase to produce cAMP that activates phospholipase A(2) (PLA(2) ) and phospholipase C (PLC) generating lysophosphatidyl choline, 1,2-diacylglycerol (DAG) and IP(3) , acting as intracellular secondary messengers required for sperm capacitation. Present study was designed to determine levels of intracellular calcium, cAMP and DAG in fresh and frozen-thawed buffalo spermatozoa cryopreserved in the presence and absence of taurine or trehalose. A total number of nine ejaculates from three randomly chosen buffalo bulls were cryopreserved in Tris-based egg yolk extender and thawed in warm water at 37°C. The cAMP was measured by enzyme immuno assay, and intracellular calcium was quantified using fluorescent dye FURA 2-AM. Total lipid was extracted from spermatozoa, and DAG was estimated using thin layer chromatography followed by spectrophotometric analysis. Intracellular calcium, cAMP and DAG levels in spermatozoa were significantly (p < 0.01) increased following cryopreservation as compared to fresh ejaculate. Addition of taurine or trehalose to the freezing medium significantly decreased (p < 0.01) the levels of intracellular calcium and cAMP in frozen-thawed spermatozoa. 1,2-diacylglycerol content was also decreased significantly (p < 0.01) in spermatozoa cryopreserved in presence of additives. Moreover, significant (p < 0.01) improvement in post-thaw motility, viability and membrane integrity of spermatozoa on addition of taurine or trehalose clearly indicated the reduced level of capacitation-like changes in buffalo spermatozoa.

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Effects of taurine and hypotaurine supplementation and ionophore concentrations on post-thaw acrosome reaction of dog spermatozoa

Cited by 22 publications

References 35 publications

Effects of Semen Extenders and Storage Temperatures on Characteristics of Frozen-Thawed Bryde's (Balaenoptera edeni) Whale Spermatozoa

Effects of Semen Extenders and Storage Temperatures on Characteristics of Frozen-Thawed Bryde's (Balaenoptera edeni) Whale Spermatozoa

Effect of Incorporation of Additives in Tris‐Based Egg Yolk Extender on Buffalo (Bubalus bubalis) Sperm Tyrosine Phosphorylation During Cryopreservation

Assessment of Intracellular Ca²⁺, cAMP and 1,2‐Diacylglycerol in Cryopreserved Buffalo (Bubalus bubalis) Spermatozoa on Supplementation of Taurine and Trehalose in the Extender

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Effects of taurine and hypotaurine supplementation and ionophore concentrations on post-thaw acrosome reaction of dog spermatozoa

Cited by 22 publications

References 35 publications

Effects of Semen Extenders and Storage Temperatures on Characteristics of Frozen-Thawed Bryde's (Balaenoptera edeni) Whale Spermatozoa

Effects of Semen Extenders and Storage Temperatures on Characteristics of Frozen-Thawed Bryde's (Balaenoptera edeni) Whale Spermatozoa

Effect of Incorporation of Additives in Tris‐Based Egg Yolk Extender on Buffalo (Bubalus bubalis) Sperm Tyrosine Phosphorylation During Cryopreservation

Assessment of Intracellular Ca2+, cAMP and 1,2‐Diacylglycerol in Cryopreserved Buffalo (Bubalus bubalis) Spermatozoa on Supplementation of Taurine and Trehalose in the Extender

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Assessment of Intracellular Ca²⁺, cAMP and 1,2‐Diacylglycerol in Cryopreserved Buffalo (Bubalus bubalis) Spermatozoa on Supplementation of Taurine and Trehalose in the Extender