Effects of taraxerol and taraxeryl acetate on cell cycle and apoptosis of human gastric epithelial cell line AGS

Tan, Bao; Shi, Hailian; Ji, Guang; Xie, Jianqun

doi:10.3736/jcim20110610

Cited by 23 publications

(12 citation statements)

References 4 publications

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“…The results showed that taraxerol reduced mitochondrial membrane potential dose-dependently. Previous investigations have also indicated that taraxerol prompts apoptosis in human gastric epithelial cells [18]. In the present study, it was observed that treatment of the epithelial cholesteatoma cells with taraxerol caused considerable accumulation of the cells in sub-G1 phase of the cell cycle, resulting in sub-G1 cell cycle arrest.…”

Section: Discussionsupporting

confidence: 62%

Taraxerol exerts potent anticancer effects via induction of apoptosis and inhibition of Nf-kB signalling pathway in human middle ear epithelial cholesteatoma cells

Liu¹,

Wu²,

Lin³

et al. 2018

Trop. J. Pharm Res

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Section: Discussionsupporting

confidence: 62%

Taraxerol exerts potent anticancer effects via induction of apoptosis and inhibition of Nf-kB signalling pathway in human middle ear epithelial cholesteatoma cells

Liu¹,

Wu²,

Lin³

et al. 2018

Trop. J. Pharm Res

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“…Taraxerol from other plant has already been reported to have plenty of pharmacological activities like antitumor, antiinflammatory, antiproliferative, and antioxidant etc. [ 13 – 16 ] These reports suggest that C. ternatea may also have potential therapeutic application over skin wound due to the presence of taraxerol. A better separation of the taraxerol marker in the extract was noted by the peak purity analysis.…”

Section: Discussionmentioning

confidence: 99%

Standardized Clitoria ternatea leaf extract as hyaluronidase, elastase and matrix-metalloproteinase-1 inhibitor

et al. 2012

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Aim:Plant Clitoria ternatea L. is claimed to possess a wide range of activities including antiinflammatory, local anesthetic and antidiabetic effect, etc. The aim of the present study was to evaluate the wound healing potential of standardized C. ternatea leaf extract in terms of different enzymatic models, which are mostly associated with skin wound.Materials and Methods:The methanol extract and fractions were screened for its hyaluronidase, elastase, and matrix metalloproteinase-1 (MMP-1) inhibitory activity compared with standard oleanolic acid. The activity was rationalized through reverse phase high performance liquid chromatography (RP-HPLC) standardization of the extract and fractions with respect to its isolated biomarker taraxerol (yield 5.27% w/w).Results:The extract showed significant (P < 0.001) hyaluronidase (IC50 18.08 ± 0.46 μg/ ml) and MMP-1 (P < 0.05) inhibition, but the elastase inhibition was insignificant (IC50 42.68 ± 0.46 μg/ml). Among the fractions, ethyl acetate fraction showed significant (P < 0.001) inhibition of hyaluronidase (IC50 28.01 ± 0.48 μg/ml) and MMP-1 (P < 0.01). The HPLC analysis revealed that the extract and the ethyl acetate fraction are enriched with taraxerol (5.32% w/w and 4.55% w/w, respectively).Conclusions:The experiment validated the traditional uses of C. ternatea and may be recommended for use in the treatment of different types of skin wounds, where taraxerol may be a responsible biomarker.

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“…The compound euphol selectively inhibits human gastric cancer cell growth through the induction of ERK1/2‐mediated apoptosis (Lin et al, ). Taraxerol also showed induce G2/M phase arrested and apoptosis in human gastric epithelial cell line (Tan et al, ). In preliminary studies, EA‐induced S‐phase cell cycle arrest led to inhibit cancer cell proliferation.…”

Section: Introductionmentioning

confidence: 99%

Latex ofEuphorbia antiquorum-induced S-phase arrest via active ATM kinase and MAPK pathways in human cervical cancer HeLa cells

et al. 2014

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Latex of Euphorbia antiquorum (EA) has demonstrated great chemotherapeutic potential for cancer. However, the mechanisms of anti-proliferation of EA on cancer cell remain to be further investigated. The purpose of this study was to explore the influence of EA in human cervical cancer cells. Here, the cell cycle distribution by flow cytometry was examined and the protein expression by the western blotting methods was analyzed. From the cytometric results it was shown that EA-induced S-phase arrest in a concentration manner both in human cervical cancer HeLa and CaSki cells. According the western blot results it was illustrated that EA could downregulate early cyclin E1-Cdk2; and cyclin A-Cdc2 provides a significant additional quantity of S-phase promotion, that in turn promoted the expression of p21(waf1/cip1) and p27(kip1) which were the inhibitors in the complex of cyclin A and Cdc2 that led to cell cycle arrest. Moreover, EA promoted the activation of ataxia telangiectasia mutated (ATM) and check-point kinase-2 (Chk2); however, it negatively regulated the expression of Topoisomerases I and II, Cdc25A, and Cdc25C signaling. Caffeine, an ATM/ATR inhibitor significantly reversed EA downregulation in the levels of Cdc25A. Furthermore, JNK inhibitor SP600125 and p38 MAPK inhibitor SB203580 both could reverse the EA upregulation of the protein of Chk2 level, significantly. This study, therefore, revealed that EA could downregulate topoisomerase, and activate ATM kinase, which then induce parallel Chk 1/2 and MAPK signaling pathways to promote the degradation of Cdc25A to induced S-phase arrest in human cervical cancer HeLa cells.

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Effects of taraxerol and taraxeryl acetate on cell cycle and apoptosis of human gastric epithelial cell line AGS

Abstract: Taraxerol has inhibitory effects on AGS cell growth through inducing G(2)/M arrest and promotion of cell apoptosis. Taraxerol acetate has less effect on cell cycle arrest and apoptosis of AGS cells than taraxerol.

Cited by 23 publications

References 4 publications

Taraxerol exerts potent anticancer effects via induction of apoptosis and inhibition of Nf-kB signalling pathway in human middle ear epithelial cholesteatoma cells

Taraxerol exerts potent anticancer effects via induction of apoptosis and inhibition of Nf-kB signalling pathway in human middle ear epithelial cholesteatoma cells

Standardized Clitoria ternatea leaf extract as hyaluronidase, elastase and matrix-metalloproteinase-1 inhibitor

Latex ofEuphorbia antiquorum-induced S-phase arrest via active ATM kinase and MAPK pathways in human cervical cancer HeLa cells

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