Effects of taraxasterol on iNOS and COX-2 expression in LPS-induced RAW 264.7 macrophages

Xiong, Huanzhang; Cheng, Yao; Zhang, Xian; Zhang, Xuemei

doi:10.1016/j.jep.2014.06.023

Cited by 64 publications

(36 citation statements)

References 42 publications

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“…Our series of studies have shown that taraxasterol has great anti-inflammatory effects in vitro and in vivo. Namely, taraxasterol exerts the in vitro anti-inflammatory and immunomodulatory activity by inhibiting inflammatory cytokine and mediator production via regulating NF-jB and MAPKs signalling pathways [12,13], and exhibits the in vivo protective effects in various animal models of inflammation including LPS-induced mouse endotoxic shock [14], ovalbumin-induced mouse allergic asthma [15] and adjuvantinduced rat arthritis [16]. Our recent study has also shown that taraxasterol possesses the protective effects on alcoholic liver injury in mice by exerting anti-oxidative stress and antiinflammatory response via CYP2E1/Nrf2/HO-1 and NF-jB signalling pathways [17].…”

Section: Introductionmentioning

confidence: 99%

Taraxasterol from Taraxacum prevents concanavalin A-induced acute hepatic injury in mice via modulating TLRs/NF-κB and Bax/Bc1-2 signalling pathways

Sang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Immune hepatic injury is a liver disease closely related to an immune imbalance of T cells and macrophages. Our previous series of studies have demonstrated that taraxasterol isolated from Taraxacum possesses great anti-inflammatory and immunomodulatory effects in vivo and in vitro. In this study, we explored the preventive effects of taraxasterol and its underlying mechanisms on concanavalin A (Con A)-induced acute hepatic injury in mice. It was found that treatment with taraxasterol significantly decreased the Con A-induced increase of liver index, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and hepatic malondialdehyde (MDA) levels, and increased the Con A-induced decrease of hepatic glutathione (GSH) and superoxide dismutase (SOD) production. Taraxasterol also significantly inhibited the release of pro-inflammatory cytokines tumour necrosis factor-a (TNF-a), interleukin-6 (IL-6), IL-1b, interferon-c (IFN-c) and IL-4. In addition, treatment with taraxasterol alleviated the hepatic histopathological injury and apoptosis induced by Con A. Furthermore, taraxasterol dramatically down-regulated the expressions of T toll-like receptor (TLR2), TLR4 and nuclear factor-jappaB (NF-jB) p65, and decreased the expression ratio of Bax/Bc1-2 in hepatic tissues. These findings suggest that taraxasterol prevents Con A-induced acute hepatic injury in mice by inhibiting TLRs/NF-jB inflammatory signalling pathway and promoting Bax/Bc1-2 anti-apoptotic signalling pathway.

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Section: Introductionmentioning

confidence: 99%

Taraxasterol from Taraxacum prevents concanavalin A-induced acute hepatic injury in mice via modulating TLRs/NF-κB and Bax/Bc1-2 signalling pathways

Sang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…In the present study, NO, PGE2 and COX-2 protein expression levels were significantly reduced by treatment with taraxasterol in a mouse model of rheumatoid arthritis. Furthermore, Xiong et al indicated that taraxasterol treatment weakens LPS-induced effects on RAW 264.7 macrophages and reduces iNOS and COX-2 expression (25). In addition, Piao et al revealed that taraxasterol suppresses PGE2 and NO production in human osteoarthritic chondrocytes (6).…”

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confidence: 99%

Protective effect of taraxasterol against rheumatoid arthritis by the modulation of inflammatory responses in mice

Shuhua

Ping

Sun

et al. 2016

Experimental and Therapeutic Medicine

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Taraxasterol is an effective component of dandelion that has anti-inflammatory effects and. The present study was performed to explore whether taraxasterol exhibits a protective effect against rheumatoid arthritis through the modulation of inflammatory responses in mice. Eight-week-old CCR9-deficient mice were injected with a collagen II monoclonal antibody cocktail to create a rheumatoid arthritis model. In the experimental group, arthritic model mice were treated with 10 mg/kg taraxasterol once per day for 5 days. Treatment with taraxasterol significantly increased the pain thresholds and reduced the clinical arthritic scores of the mice in the experimental group compared with those of the model group. Furthermore, treatment with taraxasterol significantly suppressed tumor necrosis factor-α, interleukin (IL)-1β, IL-6 and nuclear factor-κB protein expression levels compared with those in the rheumatoid arthritis model mice. Taraxasterol treatment also significantly reduced nitric oxide, prostaglandin E2 and cyclooxygenase-2 levels compared with those in the rheumatoid arthritis model group. These observations indicate that the protective effect of taraxasterol against rheumatoid arthritis is mediated via the modulation of inflammatory responses in mice.

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“…[48,49]. NO production as well as iNOS and COX-2 expression increase after stimulation with LPS [50][51][52]. Also, previous studies have reported that iNOS and COX-2 expression were suppressed by bioactive substance [31,32].…”

Section: Discussionmentioning

confidence: 92%

Bioconversion ofGentiana scabraBunge increases the anti-inflammatory effect in RAW 264.7 cells via MAP kinases and NF-κB pathway

Kim

Lee

Chon

et al. 2019

JABC

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Mitogen-activated protein (MAP) kinases play an important role in cell growth and differentiation, as well as the modulation of proinflammatory cytokines. The objective of this study was to examine the increase in the anti-inflammatory effect of Gentiana scabra Bunge (GSB), due to bioconversion with the Aspergillus kawachii crude enzyme, via inhibition of the NF-κB signaling and MAP kinase pathways in RAW 264.7 cells. The expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 in RAW 264.7 cells treated with the GSB ethyl acetate fraction bioconverted with A. kawachii crude enzyme (GE-BA), was dramatically suppressed as compared to GSB ethyl acetate fraction non-bioconverted with the A. kawachii crude enzyme (GE-UA). The phosphorylation of p38, extracellular signalregulated kinases, and inhibitory κB in RAW 264.7 cells treated with GE-BA was further suppressed, as compared to exposure to GE-UA. Moreover, the mRNA expression of interleukin 6, interleukin 1-beta, and tumor necrosis factor-α was further suppressed by GE-BA, compared to GE-UA. Similarly, antioxidant activities, such as 2,2-diphenyl-1-picrylhydrazyl hydrate and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity, of GE-BA were further increased compared to GE-UA. These observations demonstrate that the anti-oxidant and anti-inflammatory activities of GSB ethyl acetate fraction increases as a result from bioconversion with the A. kawachii crude enzyme.

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Effects of taraxasterol on iNOS and COX-2 expression in LPS-induced RAW 264.7 macrophages

Cited by 64 publications

References 42 publications

Taraxasterol from Taraxacum prevents concanavalin A-induced acute hepatic injury in mice via modulating TLRs/NF-κB and Bax/Bc1-2 signalling pathways

Taraxasterol from Taraxacum prevents concanavalin A-induced acute hepatic injury in mice via modulating TLRs/NF-κB and Bax/Bc1-2 signalling pathways

Protective effect of taraxasterol against rheumatoid arthritis by the modulation of inflammatory responses in mice

Bioconversion ofGentiana scabraBunge increases the anti-inflammatory effect in RAW 264.7 cells via MAP kinases and NF-κB pathway

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