Effects of tachyplesin and n-sodium butyrate on proliferation and gene expression of human gastric adenocarcinoma cell line BGC-823

Shi, Shengqing

doi:10.3748/wjg.v12.i11.1694

Cited by 35 publications

(15 citation statements)

References 11 publications

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“…Some reports have also shown alterations in gene expression of cancer cells in cell death process. For instance, tachyplesin decreased the expression of oncogenes C‐MYC, C‐ERB, and MTp53 in a human gastric adenocarcinoma cell line, and increased gene expression of p16 a tumor suppressor [Li et al, ; Shi et al, ]. In another study, lipopolysaccharide ‐ induced the expression of >40 genes in macrophage; the cationic AMP cecropin‐melittin selectively blocked lipopolysaccharide‐induced gene expression in murine macrophage cell line [Scott et al, ].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Cytotoxic Activity in Leukemic Lineages Reveals Important Features of β‐Hairpin Antimicrobial Peptides

Buri

Torquato

Barros

et al. 2017

J of Cellular Biochemistry

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Several reports described different modes of cell death triggered by antimicrobial peptides (AMPs) due to direct effects on membrane disruption, and more recently by apoptosis and necrosis-like patterns. Cytotoxic curves of four β-hairpin AMPs (gomesin, protegrin, tachyplesin, and polyphemusin) were obtained from several human leukemic lineages and normal monocytes and Two cell lines were then selected based on their cytotoxic sensitivity. One was sensitive to AMPs (K562) and the other resistant (KG-1) and their effect compared between these lineages. Thus, these lineages were chosen to further investigate biological features related with their cytotoxicities to AMPs. Stimulation with AMPs produced cell death, with activation of caspase-3, in K562 lineage. Increase on the fluidity of plasmatic membrane by reducing cholesterol potentiated cytotoxicity of AMPs in both lineages. Quantification of internal and external gomesin binding to the cellular membrane of both K562 and KG-1 cells showed that more peptide is accumulated inside of K562 cells. Additionally, evaluation of multi-drug resistant pumps activity showed that KG-1 has more activity than K562 lineage. A comparison of intrinsic gene patterns showed great differences between K562 and KG-1, but stimulation with gomesin promoted few changes in gene expression patterns. Differences in internalization process through the plasma membrane, multidrug resistance pumps activity, and gene expression pattern are important features to AMPs regulated cell death. J. Cell. Biochem. 118: 1764-1773, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

Comparison of Cytotoxic Activity in Leukemic Lineages Reveals Important Features of β‐Hairpin Antimicrobial Peptides

Buri

Torquato

Barros

et al. 2017

J of Cellular Biochemistry

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“…Previous studies in Caco-2 cells and lymphoma cell lines (JeKo-1, Hbl-2 and Grant-519) have reported arrests in both G 0 -G 1 and G 2 -M phases following butyrate treatment. [30][31][32][33] However, many have reported arrests in G 0 -G 1 10,14,34 or G 2 -M alone 33,35 in other cell types, such as breast and gastric cancer cells. Therefore, the timing of cell cycle arrest appears dependent on the cell line used.…”

Section: © 2 0 0 7 L a N D E S B I O S C I E N C E D O N O T D I S mentioning

confidence: 99%

Short-chain fatty acid modulation of apoptosis in the kato III human gastric carcinoma cell line

Matthews¹,

Howarth²,

Butler³

2007

Cancer Biology & Therapy

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The short-chain fatty acid (SCFA) butyrate is known to induce apoptosis in colon cancer cells in vitro and in vivo, however, its mode of action is poorly defined, whilst less is known regarding the effects of the SCFA propionate. This study investigated the potential for butyrate and propionate to alter cell viability, cell cycle regulation and intracellular protective mechanisms in a human gastric cancer cell line (Kato III). Kato III cells were incubated with butyrate or propionate for 24, 48 and 72 hr. At each time point, cells were assessed for the induction of apoptosis and cell cycle alterations using flow cytometry. Oxidative pentose pathway (OPP) activity and glutathione (GSH) availability were also measured as an index of intracellular protection. Butyrate and propionate differentially induced apoptosis and necrosis in Kato III cells and arrested cells in the G 2 -M phase. OPP activity was significantly increased by both SCFAs although butyrate induced a 10-fold greater increase than propionate. GSH availability was significantly decreased in Kato III cells by butyrate and propionate. These findings demonstrate that butyrate and propionate induce apoptosis and cell cycle alterations in Kato III gastric cancer cells. Moreover, the effects of butyrate were significantly greater than propionate. We propose that alterations to intracellular redox state and GSH availability play an important role in SCFA-mediated cell death in this cell type. The inclusion of butyrate and propionate as adjunctive cancer therapies has the potential to enhance the efficacy of current chemotherapeutics in the treatment of gastric cancer.

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“…A synthetic peptide from tachyplesin I has been demonstrated to decrease the growth of tumor cells in vitro and in vivo following linkage to the integrin homing arginine-glycine-aspartic acid (RGD) domain (3). Several studies had demonstrated that tachylesin inhibited the proliferation of tumor cells, including gastric adenocarcinoma, human hepatocarcinoma, prostate cancer and melanoma (3)(4)(5). In addition, tachyplesin I affects the differentiation of tumor cells (6).…”

Section: Introductionmentioning

confidence: 99%

Effects of tachyplesin I on human U251 glioma stem cells

Ding

Jin

Zhang

et al. 2014

Molecular Medicine Reports

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Glioblastoma, is one of the most malignant types of intracranial tumor with complex progressive cellular and underlying molecular events. The use of glioma stem cells (GSCs) offers a promising strategy for tumor therapy in the future. Tachyplesin I has been demonstrated to have potential anticancer activity and was first observed in leukocytes. In the present study, the GSC subset was isolated from U251 glioma cells and tachyplesin I was assessed for antitumor activity. As a result, the U251 cells exhibited certain GSC phenotypes, including the expression of stem cell biomarkers CD133 and nestin, when transferred into stem cell culture conditions. The GSCs were grown in an adherent manner in a medium containing serum, while the U251 glioma cells were suspended and cultured in serum‑free medium. Tachyplesin I damaged the structure of GSC and inhibited the culture of GSC spheres in a time and dose‑dependent manner. When tachyplesin I was administered at a concentration of 10‑40 µg/ml, GSC differentiation was induced. GSCs treated with a low dose of tachyplesin I disrupted the plasma membrane and led to a loss of cytoplasmic organelles. These findings indicated that tachyplesin I had an effect on inhibiting tumor stem cells and demonstrated that tachyplesin I inhibited GSCs by disrupting the plasma membranes and inducing GSC differentiation.

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Effects of tachyplesin and n-sodium butyrate on proliferation and gene expression of human gastric adenocarcinoma cell line BGC-823

Abstract: Tachyplesin as a differentiation inducer of tumor cells has similar effects as n-sodium butyrate on proliferation of tumor cells, expression of correlative oncogene and tumor suppressor gene. It also has a synergistic effect on differentiation of tumor cells.

Cited by 35 publications

References 11 publications

Comparison of Cytotoxic Activity in Leukemic Lineages Reveals Important Features of β‐Hairpin Antimicrobial Peptides

Comparison of Cytotoxic Activity in Leukemic Lineages Reveals Important Features of β‐Hairpin Antimicrobial Peptides

Short-chain fatty acid modulation of apoptosis in the kato III human gastric carcinoma cell line

Effects of tachyplesin I on human U251 glioma stem cells

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