Effects of T‐2 Toxin on Pacific White Shrimp <i>Litopenaeus vannamei</i>: Growth, and Antioxidant Defenses and Capacity and Histopathology in the Hepatopancreas

Deng, Yijia; Wang, Yaling; Zhang, Xiaodi; Sun, Lijun; Wu, Chaojin; Shi, Qi; Wang, Rundong; Sun, Xiaodong; Bi, Siyuan; Gooneratne, Ravi

doi:10.1080/08997659.2016.1249577

Cited by 21 publications

(13 citation statements)

References 66 publications

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“…T‐2 (Enzo, USA) was mixed with shrimp feed following the procedure described by Deng et al . The oral LC 50 of T‐2 for shrimp is 24.4 mg kg −1 feed dry matter (DM) .…”

Section: Methodsmentioning

confidence: 99%

“…Fifty shrimp (5.0 ± 0.5 g) from East Island (Zhanjiang, Guangdong province, China) were divided into five groups (ten shrimp / group). The rearing conditions were as described by Deng et al . At the end of the experiment, the shrimp muscles were removed for analysis.…”

Section: Methodsmentioning

confidence: 99%

“…T‐2 contamination is widespread in animal feed, including shrimp feed . In shrimp, T‐2 mostly affects protein synthesis by causing apoptosis and inhibiting protein synthesis . Long‐term exposure to T‐2 is therefore suspected to affect muscle protein quality in shrimp.…”

Section: Introductionmentioning

confidence: 99%

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Effects of T‐2 toxin on the muscle proteins of shrimp (Litopenaeus vannamei) – a proteomics study

et al. 2019

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BACKGROUND: T-2 toxin (T-2) is a potent mycotoxin and a common contaminant of aquatic animal feed, posing a serious risk to health and aquatic animals. We investigated the effect of T-2 on shrimp muscle proteins using proteomics and conventional biochemical methods. Shrimp were fed a diet containing T-2 at 0-12.2 mg kg −1 for 20 days, and changes to the muscle protein composition, ATPase activities, and the sulfhydryl (SH) content and hydrophobicity of actomyosin (AM) were determined. A proteomics study of the proteins was conducted with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional (2D) electrophoresis, and matrix-assisted laser desorption/ionization -time of flight mass spectrometry (MALDI-TOF/TOF MS). (1.2 mg kg −1 ), the levels of three major muscle proteins (myofibrillar, sarcoplasmic, and stroma) increased but at higher concentrations they declined progressively. T-2 exposure also led to a breakdown of muscle proteins as evidenced by increases in alkali-soluble protein and the surface hydrophobicity (SoANS) of AM. Thirty differentially expressed proteins were detected, 12 of which showed a concentration-response relationship with T-2 exposure. Among them, 11 homologous proteins were identified by mass spectrometry (MS), with several being key enzymes in energy metabolism. RESULTS: Exposure to T-2 markedly affected the muscle protein composition of shrimp in a concentration-responsive manner that displayed a diphasic effect. At a low T-2 concentrationCONCLUSION: This study demonstrated that T-2 exposure at medium to high concentrations could significantly affect the protein composition and quality of shrimp muscle, and potentially some of its key metabolisms. One of the arginine kinases (spot 27) was particularly responsive to T-2 and could potentially be used as a biomarker protein for T-2 intoxication by shrimp. /jsfa dine muscle stored at 0 ∘ C. J Food Sci 65:40-47 (2000).30 Tsuchiya T, Tsuchiya Y, Nonomura Y and Matsumoto JJ, Prevention of freeze denaturation of carp actomyosin by sodium glutamate.

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“…T‐2 (Enzo, USA) was mixed with shrimp feed following the procedure described by Deng et al . The oral LC 50 of T‐2 for shrimp is 24.4 mg kg −1 feed dry matter (DM) .…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Effects of T‐2 toxin on the muscle proteins of shrimp (Litopenaeus vannamei) – a proteomics study

et al. 2019

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“…The increase in thiobarbituric acid-reactive substances (TBARS) and decrease in antioxidant levels following T-2 toxin treatment may induce oxidative stress in joint tissues and contribute to the pathological process of cartilage damage in KBD. In addition, in aquatic species, T-2 toxin induces anemia and oxidative stress, as well as altering the immune response [ 50 , 51 ]. Shrimp exposed to T-2 toxin in the diet showed elevated ROS levels, SOD activity, and histopathological changes in the hepatopancreas [ 51 ].…”

Section: Oxidative Stress and Trichothecenesmentioning

confidence: 99%

Antioxidant agents against trichothecenes: new hints for oxidative stress treatment

Wang

Nepovimová

et al. 2017

Oncotarget

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Trichothecenes are a group of mycotoxins mainly produced by fungi of genus Fusarium. Due to high toxicity and widespread dissemination, T-2 toxin and deoxynivalenol (DON) are considered to be the most important compounds of this class. Trichothecenes generate free radicals, including reactive oxygen species (ROS), which induce lipid peroxidation, decrease levels of antioxidant enzymes, and ultimately lead to apoptosis. Consequently, oxidative stress is an active area of research on the toxic mechanisms of trichothecenes, and identification of antioxidant agents that could be used against trichothecenes is crucial for human health. Numerous natural compounds have been analyzed and have shown to function very effectively as antioxidants against trichothecenes. In this review, we summarize the molecular mechanisms underlying oxidative stress induced by these compounds, and discuss current knowledge regarding such antioxidant agents as vitamins, quercetin, selenium, glucomannan, nucleotides, antimicrobial peptides, bacteria, polyunsaturated fatty acids, oligosaccharides, and plant extracts. These products inhibit trichothecene-induced oxidative stress by (1) inhibiting ROS generation and induced DNA damage and lipid peroxidation; (2) increasing antioxidant enzyme activity; (3) blocking the MAPK and NF-κB signaling pathways; (4) inhibiting caspase activity and apoptosis; (5) protecting mitochondria; and (6) regulating anti-inflammatory actions. Finally, we summarize some decontamination methods, including bacterial and yeast biotransformation and degradation, as well as mycotoxin-binding agents. This review provides a comprehensive overview of antioxidant agents against trichothecenes and casts new light on the attenuation of oxidative stress.

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“…Periodically increasing doses method can monitor this accumulation process (Atchison, Yang, & Peterson, 1981), and it is increasingly used for studying the cumulative toxicity (Deng et al, 2017;Gupta, Murthy, & Chandra, 1981). The quality deterioration of shrimp muscle is closely associated with changes of color and texture, lipid oxidation, pH and freshness (K-value, total volatile basic nitrogen [TVB-N]) (Annamalai et al, 2015).…”

Section: Practical Applicationsmentioning

confidence: 99%

Evaluation the effect of mycotoxins on shrimp ( Litopenaeus vannamei ) muscle and their limited exposure dose for preserving the shrimp quality

Fang

Zhou

Wang

et al. 2019

J Food Process Preserv

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Mycotoxins widely contaminated the feed ingredients of shrimp, threatening the aquatic products, but their adverse effects on quality is less known. In this study, the adverse effects of T‐2 toxin (T‐2), aflatoxin B (AFB1), deoxynivalenol (DON), and ochratoxin (OTA) on the quality of Litopenaeus vannamei were investigated and compared. The results demonstrated that these four mycotoxins led the worse shrimp muscle color and tender texture, induced lipid peroxidation and pH change. Furthermore, four mycotoxins increased the K‐value and total volatile basic nitrogen (TVB‐N) to decrease the freshness. The median effective doses of T‐2, DON, AFB1, and OTA on TVB‐N of shrimp muscle were 4.86, 1.51, 0.26, and 0.20 mg/kg·bw, respectively. This study could provide useful information about the impacts and limited exposure dose of mycotoxins for quality preservation of shrimp. Practical applications The shrimp quality is easy to deteriorate as it is most likely to be exposed to mycotoxins because of intensive farming practices using grain‐based feeds, which were widely contaminated by mycotoxins. This study may provide a limited exposure dose of four prevailing mycotoxins aflatoxin B, deoxynivalenol, ochratoxin, and T‐2 for preserving the minimal quality of shrimp, and also provide useful information for predicting the quality of shrimp from those highly mycotoxins‐contaminated areas.

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Effects of T‐2 Toxin on Pacific White Shrimp Litopenaeus vannamei: Growth, and Antioxidant Defenses and Capacity and Histopathology in the Hepatopancreas

Cited by 21 publications

References 66 publications

Effects of T‐2 toxin on the muscle proteins of shrimp (Litopenaeus vannamei) – a proteomics study

Effects of T‐2 toxin on the muscle proteins of shrimp (Litopenaeus vannamei) – a proteomics study

Antioxidant agents against trichothecenes: new hints for oxidative stress treatment

Evaluation the effect of mycotoxins on shrimp ( Litopenaeus vannamei ) muscle and their limited exposure dose for preserving the shrimp quality

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