Effects of synthetic antifreeze glycoprotein analogue on islet cell survival and function during cryopreservation

Matsumoto, Shuichiro; Matsusita, Michiaki; Morita, Tomoya; Kamachi, Hirofumi; Tsukiyama, Shusaku; Furukawa, Yoshinori; Koshida, Shuhei; Tachibana, Yuki; Nishimura, Shin‐Ichiro; Todo, Satoru

doi:10.1016/j.cryobiol.2005.10.010

Cited by 72 publications

(53 citation statements)

References 19 publications

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“…These proteins modify the growth habits, inhibit the recrystallization and the growth of ice and have drawn a considerable interest due to their applicability in the frozen food industry, 1,2 protection of plants that have been transgenetically mutated to express these proteins, 3 as a cryosurgery adjuvant, 4 in rat islet preservation, and even the possibility of human organ preservation. 5,6 Although it is clear that the proteins carry out their functions via some surface interaction, there is still much debate as to the exact details of the mechanism. 7 Additionally, to date no thorough quantitative description of the ice crystal growth kinetics changes due to the protein interactions has been reported and such studies could lead to a better understanding of the anisotropic, binding site, or plane specific nature of the interaction.…”

Section: Introductionmentioning

confidence: 99%

Apparatus for single ice crystal growth from the melt

Zepeda

Nakatsubo

Furukawa

2009

Review of Scientific Instruments

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A crystal growth apparatus was designed and built to study the effect of growth modifiers, antifreeze proteins and antifreeze glycoproteins ͑AFGPs͒, on ice crystal growth kinetics and morphology. We used a capillary growth technique to obtain a single ice crystal with well-defined crystallographic orientation grown in AFGP solution. The basal plane was readily observed by rotation of the capillary. The main growth chamber is approximately a 0.8 ml cylindrical volume. A triple window arrangement was used to minimize temperature gradients and allow for up to 10 mm working distance objective lens. Temperature could be established to within Ϯ10 mK in as little as 3.5 min and controlled to within Ϯ2 mK after 15 min for at least 10 h. The small volume growth chamber and fast equilibration times were necessary for parabolic flight microgravity experiments. The apparatus was designed for use with inverted and side mount configurations.

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Section: Introductionmentioning

confidence: 99%

Apparatus for single ice crystal growth from the melt

Zepeda

Nakatsubo

Furukawa

2009

Review of Scientific Instruments

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“…Essentially, AF(G)Ps bind to specific planes of ice, inhibiting their growth, but upon cooling, the remaining exposed faces grow, giving rise to a spicular (needle-like) morphology causing mechanical damage 17 . Carpenter et al 11 and Matsumoto et al 18 demonstrated small improvements in cryopreservation with an AFP and an AF(G)P mimic respectively, but the benefit was limited by the low concentrations, which could be applied before cell viability was reduced because of ice-shaping. Wang et al 15 observed that AF(G)P failed to preserve rat hearts.…”

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confidence: 99%

Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing

et al. 2014

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The cryopreservation of cells, tissue and organs is fundamental to modern biotechnology, transplantation medicine and chemical biology. The current state-of-the-art method of cryopreservation is the addition of large amounts of organic solvents such as glycerol or dimethyl sulfoxide, to promote vitrification and prevent ice formation. Here we employ a synthetic, biomimetic, polymer, which is capable of slowing the growth of ice crystals in a manner similar to antifreeze (glyco)proteins to enhance the cryopreservation of sheep and human red blood cells. We find that only 0.1 wt% of the polymer is required to attain significant cell recovery post freezing, compared with over 20 wt% required for solvent-based strategies. These results demonstrate that synthetic antifreeze (glyco)protein mimics could have a crucial role in modern regenerative medicine to improve the storage and distribution of biological material for transplantation.

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“…[34] The haemolysis of red blood cells was reduced considerably at 500 mg mL À1 (12 vs 46 % in the absence of syAFGP) and attenuated at higher syAFGP concentrations.…”

Section: Natural Antifreeze Glycoproteinsmentioning

confidence: 99%

Design and Synthesis of Antifreeze Glycoproteins and Mimics

Harding

2010

ChemBioChem

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Antifreeze glycoproteins are an important class of biological antifreezes that have potential applications in many areas of medicine, agriculture and industry in which ice crystal growth is damaging. While the synthesis of antifreeze glycoproteins as pure glycoforms has recently been achieved by using ligation and polymerisation strategies, the routine production of large quantities of pure glycoforms remains challenging. A range of C-linked analogues that are readily produced by solid-phase synthesis have delivered novel compounds that are not biological antifreezes, but are potent, non-cytotoxic, ice-recrystallisation inhibitors. Structure-activity studies, the identification of cyclic antifreeze glycoproteins and conformational studies have provided further insight into the requirements for antifreeze activity. These results, coupled with significant advances in approaches to the routine synthesis of different glycoproteins and mimics, present opportunities for the design and synthesis of novel ice-growth-inhibiting and antifreeze compounds.

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Effects of synthetic antifreeze glycoprotein analogue on islet cell survival and function during cryopreservation

Cited by 72 publications

References 19 publications

Apparatus for single ice crystal growth from the melt

Apparatus for single ice crystal growth from the melt

Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing

Design and Synthesis of Antifreeze Glycoproteins and Mimics

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