Effects of surfactants on glucosyltransferase production and in vitro sucrose-dependent colonization by Streptococcus mutans

Tomita, Yukihide; Watanabe, Toshi; Takeuchi, Totaro; Nanbu, Akio; Shinozaki, Noriko; Ikemi, Takuji; Fukushima, Kazuo

doi:10.1016/s0003-9969(98)00065-x

Cited by 15 publications

(5 citation statements)

References 23 publications

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“…Biofilm formation on glass is a simple model that has been used by others to assess sucrose-dependent colonization of S. mutans [13,15,21,42]. In the current study, the biofilms were challenged by vortexing at 1200 rev/min for 10 s to show the gradual decrease in their adherence/integrity as the khat extract concentration increased.…”

Section: Discussionmentioning

confidence: 96%

In vitroeffects of crude khat extracts on the growth, colonization, and glucosyltransferases ofStreptococcus mutans

Al-Hebshi

Nielsen

Skaug

2005

Acta Odontologica Scandinavica

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Millions of Yemenites, East Africans, and immigrants to Western countries chew khat daily for its amphetamine-like effects. There is little information in the literature concerning the possible effects of the habit on oral microbiota. Our objective was to study in vitro crude khat extract effects on Streptococcus mutans growth and sucrose-dependent colonization, and on its glucosyltransferase (GTF) activity and production. Three khat cultivars were used. Lyophilized crude aqueous khat extracts were applied to the different assays at concentrations of 0-1% (w/v). Sucrose-dependent colonization was assessed as the ability of Streptococcus mutans UA159 to form adherent biofilms in glass culture tubes. Colony forming units (CFUs) in the planktonic phase served as a measure of bacterial growth, while CFUs in the biofilm phase were used to quantify viability in the biofilms. GTFs activity was tested by incubating a crude GTFs preparation with sucrose and determining the amount of water-soluble and water-insoluble glucans formed. GTFs production was assayed by comparing intensities of GTF bands in Western blots of extracts from control and khat-containing cultures. The khat extracts effectively inhibited biofilm formation. The minimum biofilm inhibitory concentration (MBIC) varied among the cultivars (0.25-1%). The extracts also inhibited synthesis of both glucan types, particularly insoluble glucans (average 85% inhibition at 1%), with significant differences among the cultivars. However, khat increased bacterial growth and at sub-MBIC also viability within biofilms; there were no inter-cultivar differences. It is shown that khat leaves contain water-soluble constituents that inhibit some cariogenic properties of S. mutans in vitro.

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Section: Discussionmentioning

confidence: 96%

In vitroeffects of crude khat extracts on the growth, colonization, and glucosyltransferases ofStreptococcus mutans

Al-Hebshi

Nielsen

Skaug

2005

Acta Odontologica Scandinavica

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“…Previous studies have described the effects of non-ionic surfactants for both the expression and the activity of GTF enzymes in S. mutans. Using monoclonal antibodies against S. mutans GTF enzymes Tomita et al [37] have reported on the effects of Tween 80 (Polyoxyethylene Sorbitan Monooleate, fatty acid esters of sorbitan polyethoxylates) and lecithin (a mixture of natural phospholipids) that increased production of glucosyltransferase and increased sucrose-dependent colonization of S. mutans. They showed that water insoluble glucan synthesis and artificial plaque formation (in vitro sucrosedependent colonization) by S. mutans PS14 were significantly enhanced by the addition of Tween 80 at concentrations over 0.00125% (0.02 mM).…”

Section: Discussionmentioning

confidence: 99%

“…They showed that water insoluble glucan synthesis and artificial plaque formation (in vitro sucrosedependent colonization) by S. mutans PS14 were significantly enhanced by the addition of Tween 80 at concentrations over 0.00125% (0.02 mM). They concluded that surfactants might vary the cariogenic potential of S. mutans even at these low concentrations [37]. Tween 80 has also been shown to be superior to sucrose in stimulating insoluble glucan synthesis through enhanced production of GTF enzymes in Streptococcus salivarius [38].…”

Section: Discussionmentioning

confidence: 99%

The influence of triethylene glycol derived from dental composite resins on the regulation of Streptococcus mutans gene expression

et al. 2009

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“…It is demonstrated that chemical surfactants exerted different effects on the synthesis of glucosyltransferases in S. mutans ; Tween 80 significantly increased the level of gtfs , while Triton X-100 decreased gtf levels. [ 37 ] In general, the data suggest that biosurfactant treatment can provide an option for controlling biofilm development and also influence the adhesive ability of bacterial pathogens. [ 38 ]…”

Section: Discussionmentioning

confidence: 99%

Effects of biosurfactant produced by Lactobacillus casei on gtfB, gtfC, and ftf gene expression level in S. mutans by real-time RT-PCR

et al. 2014

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Background:The Streptococci are the pioneer strains in plaque formation and Streptococcus mutans are the main etiological agent of dental plaque and caries. In general, biofilm formation is a step-wise process, which begins by adhesion of planktonic cells to the surfaces. Evidences show that expression of glucosyltransferase B and C (gtfB and gtfC) and fructosyltransferase (ftf) genes play critical role in initial adhesion of S. mutans to the tooth surface which results in formation of dental plaques and consequently caries and other periodontal disease.Materials and Methods:The aim of this study was to determine the effect of biosurfactants produced by a probiotic strain; Lactobacillus casei (ATCC39392) on gene expression profile of gftB/C and tft of S. mutans (ATCC35668) using quantitative real-time PCR.Results:The application of the prepared biosurfactant caused dramatic down regulation of all the three genes under study. The reduction in gene expression was statistically highly significant (for gtfB, P > 0.0002; for gtfC, P > 0.0063, and for ftf, P > 0.0057).Conclusion:Considerable downregulation of all three genes in the presence of the prepared biosurfactant comparing to untreated controls is indicative of successful inhibition of influential genes in bacterial adhesion phenomena. In view of the importance of glucosyltransferase gene products for S.mutans attachment to the tooth surface which is the initial important step in biofilm production and dental caries, further research in this field may lead to an applicable alternative for successful with least adverse side effects in dental caries prevention.

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Effects of surfactants on glucosyltransferase production and in vitro sucrose-dependent colonization by Streptococcus mutans

Cited by 15 publications

References 23 publications

In vitroeffects of crude khat extracts on the growth, colonization, and glucosyltransferases ofStreptococcus mutans

In vitroeffects of crude khat extracts on the growth, colonization, and glucosyltransferases ofStreptococcus mutans

The influence of triethylene glycol derived from dental composite resins on the regulation of Streptococcus mutans gene expression

Effects of biosurfactant produced by Lactobacillus casei on gtfB, gtfC, and ftf gene expression level in S. mutans by real-time RT-PCR

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