Abstract:Effects of supplemental xylanase on in vitro disappearance of dry matter in feed ingredients for swine This unedited manuscript has been accepted by RCCP for future publication and is provisionally published on our website. The manuscript will undergo copyediting, typesetting, and galley review before final publication. Please note that this advance version may differ from the "Ahead of print" and also from the final version. SHORT COMMUNICATION Effects of supplemental xylanase on in vitro disappearance of dry… Show more
“…Thus, the demand for alternative feed ingredients has been increased. However, majority of alternative feed ingredients originating from plants are rich in non-starch polysaccharides (NSP), which are indigestible fractions by endogenous enzymes of pigs and act as antinutritional factors during the digestion and absorption processes [4,5]. For this reason, increased inclusion rates of alternative feed ingredients in diets may result in reduced digestibility of nutrients and energy in pigs.…”
The present study was aimed to investigate the effects of supplemental β-mannanase on in vitro ileal disappearance (IVID) and in vitro total tract disappearance (IVTTD) of dry matter (DM) in barley, canola meal, copra meal, corn, corn distillers dried grains with solubles, cottonseed meal, palm kernel meal, soybean meal, and wheat for pigs. Feed ingredient samples were finely ground and divided into the control group and the treatment group. The samples of the control group were prepared to contain 990 g/kg test ingredient and 10 g/kg cornstarch, whereas the samples of the treatment group were prepared to contain 990 g/kg test ingredient and 10 g/kg β-mannanase product (8,000 units/kg in the mixed sample). A 2-step in vitro ileal digestion technique, which simulated the digestion and absorption processes in the stomach and small intestine, was used to determine the IVID of DM in test ingredients, whereas a 3-step in vitro ileal digestion technique, which additionally simulated the digestion process of the large intestine, was used to determine the IVTTD of DM in test ingredients. The in vitro digestion procedures were performed in triplicate for each sample. The addition of βmannanase increased (p = 0.003) the IVID of DM in wheat and tended to increase (p = 0.063) the IVID of DM in soybean meal. The IVTTD of DM in barley, cottonseed meal, and palm kernel meal was improved (p < 0.05) by the addition of β-mannanase. In conclusion, the digestibility of nutrients for pigs may be improved when β-mannanase is added into diets containing barley, cottonseed meal, palm kernel meal, soybean meal, or wheat.
“…Thus, the demand for alternative feed ingredients has been increased. However, majority of alternative feed ingredients originating from plants are rich in non-starch polysaccharides (NSP), which are indigestible fractions by endogenous enzymes of pigs and act as antinutritional factors during the digestion and absorption processes [4,5]. For this reason, increased inclusion rates of alternative feed ingredients in diets may result in reduced digestibility of nutrients and energy in pigs.…”
The present study was aimed to investigate the effects of supplemental β-mannanase on in vitro ileal disappearance (IVID) and in vitro total tract disappearance (IVTTD) of dry matter (DM) in barley, canola meal, copra meal, corn, corn distillers dried grains with solubles, cottonseed meal, palm kernel meal, soybean meal, and wheat for pigs. Feed ingredient samples were finely ground and divided into the control group and the treatment group. The samples of the control group were prepared to contain 990 g/kg test ingredient and 10 g/kg cornstarch, whereas the samples of the treatment group were prepared to contain 990 g/kg test ingredient and 10 g/kg β-mannanase product (8,000 units/kg in the mixed sample). A 2-step in vitro ileal digestion technique, which simulated the digestion and absorption processes in the stomach and small intestine, was used to determine the IVID of DM in test ingredients, whereas a 3-step in vitro ileal digestion technique, which additionally simulated the digestion process of the large intestine, was used to determine the IVTTD of DM in test ingredients. The in vitro digestion procedures were performed in triplicate for each sample. The addition of βmannanase increased (p = 0.003) the IVID of DM in wheat and tended to increase (p = 0.063) the IVID of DM in soybean meal. The IVTTD of DM in barley, cottonseed meal, and palm kernel meal was improved (p < 0.05) by the addition of β-mannanase. In conclusion, the digestibility of nutrients for pigs may be improved when β-mannanase is added into diets containing barley, cottonseed meal, palm kernel meal, soybean meal, or wheat.
“…In vitro assays can be used for estimating the nutrient availability of non-ruminants [ 15 , 17 , 26 , 27 ]. In this study, a two-step in vitro procedure for dogs was adopted, which was modified from the procedure used for pigs by lowering the doses of exogenous digestive enzymes considering the shorter gastrointestinal tract and higher passage rate of digestion in dogs compared with pigs [ 15 ].…”
The aims of this study were to determine in vitro ileal disappearance (IVID) of dry matter (DM) and crude protein (CP) in commercial dog diets and to develop equations for predicting the IVID of DM and CP in dog diets based on chemical composition. Eighteen commercial dog diets were analyzed for IVID of DM and CP using a two-step in vitro procedure for dogs. The diet samples in flasks with digestive enzymes were incubated for 2 h and 4 h to simulate digestion in the stomach and the small intestine, respectively. The contents of CP, ether extract, neutral detergent fiber (NDF), and ash in the diets ranged from 14.4 to 42.5%, 3.5 to 23.5%, 6.4 to 34.6%, and 4.9 to 10.0%, respectively, on an as-is basis. The NDF contents were negatively correlated with the IVID of DM and CP (r = −0.73 and r = −0.62, respectively; p < 0.05). The most suitable prediction equations for the IVID of DM and CP in the dog diets were: IVID of DM (%) = 81.33 + 0.46 × CP − 0.77 × NDF, R2 = 0.78; IVID of CP (%) = 81.25 + 0.33 × CP − 0.49 × NDF, R2 = 0.64, where all nutrients were in % on an as-is basis. In conclusion, dry matter and protein utilization of dog diets based on in vitro digestibility assays can be estimated fairly well using protein and fiber concentrations as independent variables.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.