Effects of superoxide dismutase on mouse in vitro fertilization and embryo culture system

Nonogaki, Takafumi; Noda, Yoichi; Narimoto, Kazutaka; Umaoka, Yoh; Mori, Takahide

doi:10.1007/bf01203828

Cited by 36 publications

(12 citation statements)

References 25 publications

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“…This result is consistent with previous observations (Loutradis et al 1987;Nureddin et al 1990; Bastias et al 1993). Free oxygen radicals have been shown to be generated from xanthine oxidase and HX reactions under embryo culture conditions (Goto et al 1993;Iwata et al 1999); moreover, free oxygen radicals (high O 2 tension) negatively affect embryo development (Nonogaki et al 1992;Takahashi et al 1996). In the present study, we have observed no detrimental effect of HX when embryos are cocultured with goat OECs, especially with 1-day confluent monolayers, and HX concentrations do not differ significantly in the medium.…”

Section: Discussionsupporting

confidence: 95%

Effects of species and cellular activity of oviductal epithelial cells on their dialogue with co-cultured mouse embryos

Tan

et al. 2006

Cell Tissue Res

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An efficient co-culture system, especially with oviductal or uterine epithelial cells, is important not only for the production of high quality embryos, but also for the study of the molecular dialogue between embryos and their maternal environment. Although mouse embryos have been co-cultured successfully with oviductal epithelial cells (OECs) from several species, studies on the effects of species and functionality of OECs are few. Reports concerning the necessity of direct contact between the embryo and OECs and about the culture of mouse embryos in medium conditioned with heterologous OECs have been controversial. In this study, pronuclear embryos from Kunming mice, characterized by an obvious two-cell block in vitro, were co-cultured with mouse, goat, and chick OECs. The functionality of OECs was determined by analyzing the cell cycle, apoptosis, the numbers of mitochondria and cilia, and the ability both to support embryonic development and to remove hypoxanthine from the culture medium. The necessity of direct contact between OECs and embryos was studied by repeated renewal of culture medium with fresh conditioned medium, the culture of embryos in plastic wells connected by tunnels to wells with OEC monolayers, and the co-culture of embryos separated from OECs by a filter. Both goat and chick OECs supported mouse embryonic development, but their embryotrophic lifespan was shorter than that of the mouse OECs. Whereas media conditioned with mouse OECs supported mouse embryonic development satisfactorily, medium conditioned with goat OECs supported little development. Immediate dialogue between heterologous OECs and embryos was essential for efficient co-culture, whereas direct contact between the two cell types was not; neither dialogue nor contact was needed between isologous OECs and embryos. Embryotrophic activity and the ability to remove hypoxanthine from conditioned medium declined with time after confluence and number of passages of OECs, mainly because of apoptosis and dedifferentiation. Thus, the species and functionality of OECs have profound effects on their molecular dialogue with co-cultured embryos, and efficient co-culture depends upon both positive and negative conditioning.

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Section: Discussionsupporting

confidence: 95%

Effects of species and cellular activity of oviductal epithelial cells on their dialogue with co-cultured mouse embryos

Tan

et al. 2006

Cell Tissue Res

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“…It is interesting to observe that blastocyst development in vitro always lags behind blastocyst development in vivo, as there is a variation in the ability of IVF media and its components to scavenge ROS and prevent DNA damage. Addition of the enzymatic antioxidant, for example SOD, to the culture medium prevented the deleterious effects of oxidative stress on sperm viability and on the embryo development both in vivo and in vitro [85]. This effect was demonstrated by increased development of two-cell stage embryos to the expanded blastocyst stage in the SOD-supplemented medium.…”

Section: Role Of Antioxidant Supplementationmentioning

confidence: 87%

The role of free radicals and antioxidants in reproduction

Agarwal

Gupta

Sikka

2006

Current Opinion in Obstetrics &Amp; Gynecology

395

277

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Oxidative stress has been implicated in male and female infertility, including fetal dysmorphogenesis, abortions, and intrauterine growth restriction. Accurate evaluation of seminal oxidative stress by standardized assays may help in the diagnosis and management of male infertility. There is evidence in the literature on the beneficial effects of oral antioxidant supplementation in male infertility. Current ongoing trials will provide answers on the safety and effectiveness of antioxidants in improving maternal and fetal outcomes. Further studies need to be conducted to determine if antioxidant supplementation will prevent fetal developmental defects in high-risk pregnancy with diabetes.

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“…However, additional extracellular protection against superoxide anion toxicity seems also to be required in vivo as well as in vitro since Cu/Zn SOD has been found in oviduct and uterine secretion in the mouse (Chun et al, 1994) and rabbit . Several studies have shown that supplementation of culture media with Cu/Zn SOD had a favorable effect on embryo development in bovine (Lauria et al, 1994;Fujitani et al, 1997), mouse Nonogaki et al, 1992) and rabbit (Li et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Expression of Cu/Zn and Mn superoxide dismutases during bovine embryo development: Influence of in vitro culture

et al. 2000

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Temporal pattern of expression of Cu/Zn and Mn superoxide dismutases (SODs) was investigated in bovine oocytes and embryos produced in vitro in two different culture conditions and in vivo after superovulation. SODs were examined at a transcriptional level in single oocytes and embryos by reverse transcriptase±polymerase chain reaction (RT-PCR) and, at a protein level, by Western blotting on pools of embryos. mRNA encoding Cu/Zn SOD were detected in in vitro bovine embryos throughout preattachment development as well as in in vivo derived morulae and blastocysts. Transcripts for Mn SOD gene were detected in most immature and in vitro matured oocytes as well as in some zygotes and 5-to 8-cell embryos while no transcript was found at the 9to 16-cell stage in both culture conditions. In vitro embryonic expression of Mn SOD was detected earlier in the presence of serum. Half of the morulae showed the transcript if cultured with 5% serum while none without serum. At the blastocyst stage Mn SOD could be detected independently of culture conditions. For in vivo±derived embryos Mn SOD transcripts were detected both in morulae and blastocysts. Immunoblotting analyses revealed that Cu/Zn SOD and Mn SOD were also present at a protein level in in vitroderived zygotes and blastocysts. Together these data demonstrate, for the first time, that Mn SOD is transcribed and that Cu/Zn and Mn SOD proteins are expressed in preimplantation bovine embryos. Finally, they suggest that Mn SOD transcription is altered by in vitro culture conditions. Mol. Reprod. Dev. 58:45± 53,

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Effects of superoxide dismutase on mouse in vitro fertilization and embryo culture system

Abstract: These results indicate that the addition of SOD exerts a protecting effect from oxidative stress both on sperm viability and on the development of embryos fertilized in vitro as well as in vivo, while its addition showed no effect on the process of fertilization.

Cited by 36 publications

References 25 publications

Effects of species and cellular activity of oviductal epithelial cells on their dialogue with co-cultured mouse embryos

Effects of species and cellular activity of oviductal epithelial cells on their dialogue with co-cultured mouse embryos

The role of free radicals and antioxidants in reproduction

Expression of Cu/Zn and Mn superoxide dismutases during bovine embryo development: Influence of in vitro culture

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