Effects of Sumsu (Bufonis venenum) Pharmacopuncture Treatment on Depression in Mice

Choi, Minsu; Kim, Ka-Na; Lee, Jae-Eun; Suh, Jung Ho; Kim, Sung Chul; Kwon, Ki Rok; Cho, Seung-Hun

doi:10.3831/kpi.2014.17.013

Cited by 6 publications

(6 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Because the BVP in this test was made through hot water extraction, the components contained mainly serotonin. Serotonin (5-hydroxytryptamine) is also one of the main ingredients in BV [ 10 ]. It is involved in various psychiatric disorders such as depression, anxiety, obsessive symptoms and impulsivity, as well as in the regulation of the feeling of satiety [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…It is involved in various psychiatric disorders such as depression, anxiety, obsessive symptoms and impulsivity, as well as in the regulation of the feeling of satiety [ 11 ]. Therefore, BVP has therapeutic potential for treating patients with neuropsychiatric disorders such as anxiety or depression disorder [ 10 ]. Serotonin is one of the most effective pruritogens in the cheek model in rats.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Study of Intravenous Single-Dose Toxicity Test of Bufonis venonum Pharmacopuncture in Sprague-Dawley Rats

Kwon¹,

Yu²,

Sun³

et al. 2016

J. Pharmacopunct

View full text Add to dashboard Cite

Objectives:Bufonis venonum (BV) is toad venom and is the dried, white secretions of the auricular and the skin glands of toads. This study was performed to evaluate the toxicity of intravenous injection of Bufonis venonum pharmacopuncture (BVP) through a single- dose test with sprague-dawley (SD) rats.Methods:Twenty male and 20 female 6-week-old SD rats were injected intravenously in the caudal vein with BVP or normal saline. The animals were divided into four groups with five female and five male rats per group: the control group injected with normal saline, the low-dosage group injected with 0.1 mL/animal of BVP, the medium-dosage group injected with 0.5 mL/ animal of BVP and the high-dosage group injected with 1.0 mL/animal of BVP. We performed clinical observations every day and body weight measurements on days 3, 7 and 14 after the injection. We also conducted hematology, serum biochemistry, and histological observations immediately after the observation period.Results:No mortalities were observed in any experimental group. Paleness occurred in the medium- and the high-dosage groups, and congestion on tails was observed in females in the medium- and the high-dosage groups. No significant changes in weight, hematology, serum biochemistry, and histological observations that could be attributed to the intravenous injection of BVP were observed in any experimental group.Conclusion:The lethal dose of intravenously-administered BVP in SD rats is over 1.0 mL/animal.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Study of Intravenous Single-Dose Toxicity Test of Bufonis venonum Pharmacopuncture in Sprague-Dawley Rats

Kwon¹,

Yu²,

Sun³

et al. 2016

J. Pharmacopunct

View full text Add to dashboard Cite

show abstract

“…Biogenic amines include serotonin, histamine, dopamine, adrenaline, and noradrenaline. Toad's glandular secretions have been used for diseases such as depression, inflammation, memory, learning, and neurologic diseases such as AD and Parkinson's disease ( 17 18 19 ).…”

Section: Introductionmentioning

confidence: 99%

Bufo viridis secretions improve anxiety and depression-like behavior following intracerebroventricular injection of amyloid β

et al. 2020

View full text Add to dashboard Cite

Background and purpose: Venenum Bufonis is a Chinese traditional medicine produced from the glandular secretions of toads that contain biogenic amines, which have anti-inflammatory properties. The present study aimed to examine the effect of Bufo viridis secretions (BVS) on anxiety and depression-like behavior and hippocampal senile plaques volume in an animal model of Alzheimer's disease (AD). Experimental approach: Thirty-eight male Wistar rats were used. AD was induced by amyloid-beta (Aβ 1-42 ) (10 μg/2 μL, intracerebroventricular injection, icv) and then BVS at 20, 40, and 80 mg/kg were injected intraperitoneally (ip) in six equal intervals over 21 days. Anxiety and depression-like behavior were assessed using behavioral tests including open field test (OFT), elevated plus maze (EPM), and forced swimming test (FST) 21 days after the surgery. The volume of senile plaques was assessed based on the Cavalieri principle. Findings/Results: Results of the OFT showed that the central crossing number and the time in the AD group were significantly decreased compared to the sham group ( P < 0.01 and P < 0.001, respectively). Also, the values of these two parameters significantly increased in the AD + BVS80 group than the AD group ( P < 0.05 and P < 0.001, respectively). The time spent in the closed arm in the EPM dramatically increased in the AD group compared to the sham group ( P < 0.05) and significantly decreased in the AD + BVS80 group compared to the AD group ( P < 0.05). Results of the FST indicated that immobility time had a reduction in the AD + BVS20 ( P < 0.01), AD + BVS40, and AD + BVS80 groups compared to the AD group ( P < 0.001). The volume of senile plaques in the hippocampus showed a reduction in the treatment groups in comparison with the AD group ( P < 0.001 for all). Conclusion and implications: Results revealed that BVS injection could improve symptoms of anxiety and depression and decrease senile plaques in the hippocampus in an animal model of AD.

show abstract

“…Choi et al [9] reported recently that BVP had therapeutic potential for treating neuropsychiatric disorders such as anxiety or depression disorder, but no side effects or toxicity of BVP have been reported so far. For that reason, we conducted an intramuscular single-dose toxicity test of BVP in Sprague-Dawley (SD) rats to determine the safety of its use safe and to estimating its appropriate dosage.…”

Section: Introductionmentioning

confidence: 99%

Intramuscular Single-dose Toxicity Test of Bufonis venonum Pharmacopuncture in Sprague-Dawley Rats

Lee

Sun

et al. 2015

J. Pharmacopunct

View full text Add to dashboard Cite

Objectives:Bufonis venonum (BV) is the dried white secretions of the auricular and skin glands of the toads Bufo bufo gargarizans or Bufo melanosticus Schneider. This study was performed to evaluate the toxicity of intramuscularly- administered Bufonis venonum pharmacopuncture (BVP) and to calculate its approximate lethality through a single-dose test with Sprague-Dawley (SD) rats.Methods:Twenty male and 20 female 6-week-old SD rats were injected intramuscularly with BVP or normal saline. The animals were divided into four groups with five female and five male rats per group: the control group injected with normal saline at 0.5 mL/animal, the low-dosage group injected with 0.125 mL/animal of BVP, the medium-dosage group injected with 0.25 mL/animal of BVP and the high-dosage group injected with 0.5 mL/animal of BVP. All injections were in the left thighs of the rats. After administration, we conducted clinical observations everyday and body weight measurements on days 3, 7 and 14 after the injection. We also carried out hematology, serum biochemistry, and histological observations on day 15 after treatment.Results:No mortalities were observed in any experimental group. No significant changes in weight, hematology, serum biochemistry, and histological observations that could be attributed to the intramuscular injection of BVP were observed in any experimental group.Conclusion:Lethal dose of BVP administered via intramuscular injection in SD rats is over 0.5 mL/animal.

show abstract

Effects of Sumsu (Bufonis venenum) Pharmacopuncture Treatment on Depression in Mice

Cited by 6 publications

References 37 publications

Study of Intravenous Single-Dose Toxicity Test of Bufonis venonum Pharmacopuncture in Sprague-Dawley Rats

Study of Intravenous Single-Dose Toxicity Test of Bufonis venonum Pharmacopuncture in Sprague-Dawley Rats

Bufo viridis secretions improve anxiety and depression-like behavior following intracerebroventricular injection of amyloid β

Intramuscular Single-dose Toxicity Test of Bufonis venonum Pharmacopuncture in Sprague-Dawley Rats

Contact Info

Product

Resources

About