Effects of Sulphydryl Reagents on Receptor-Mediated Hormonal Responses at the Cellular Level: Insulin-mimicking Characteristics of Thiol-blocking Compounds in Rat Hepatocyte Primary Culture.

Abstract

The interference of various SH-blocking chemicals with the insulin-controlled regulation of the hepatic carbohydrate metabolism was studied in rat hepatocyte primary cultures. The organic mercurials PCMB, PCMBS,mersalyl the disulphide agents DTP, CPDS, disulfiram and the SH-alkylating reagent NEM were used as experimental SH-blocking model compounds. All studied compounds, except for NEM,induced an increased glycogen deposition comparable with the physiological insulin-induced glycogen-deposition. PCMBSappeared to be the most effective insulin-mimicking anabolic trigger. The action of the insulin molecule itself was potentiated by PCMBSas well as demonstrated by increased glycogen deposition, induced pyruvate kinase (PK) and decreased phosphoenol-pyruvate carboxykinase (PEP-CK) activity. However, cell-exposure to insulin and PCMBSin relatively high doses was destructive, as demonstrated by decreased glycogen levels, most probably as a result of insulin-receptor overstimulation and metabolic stress. Thus, SH-blocking compounds are able to trigger insulin-dependent metabolic processes. The relatively non-permeantorganic mercurial PCMBSproved to be the most effective insulin-mimicking SH-blocking compound.