Effects of sulfide on the integration of denitrification with anaerobic digestion

Yin, Zhixuan; Xie, Lin; Zhou, Qi

doi:10.1016/j.jbiosc.2015.02.004

Cited by 13 publications

(7 citation statements)

References 23 publications

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“…Sulfide has been shown to increase DNRA in both natural (Brunet and Garcia‐Gil, 1996; Jones et al ., 2017; Murphy et al ., 2020) and engineered systems (Dolejs et al ., 2014; Yin et al ., 2015). As discussed earlier, sulfide acts as a reducing agent, and electron‐rich environments are more prone to DNRA (van den Berg et al ., 2015).…”

Section: Resultsmentioning

confidence: 99%

“…From a treatment standpoint, the goal of biological wastewater treatment processes is to reduce nitrite to nitrogen gas instead of ammonia. Previous literature in both pure cultures and mixed wastewater systems found that at higher sulfide:nitrogen (S:N) ratio, nitrate is reduced to ammonia, while at lower S:N ratios denitrification occurs (Dolejs et al ., 2014; Yin et al ., 2015). The ratio of S:N that induced DNRA in these studies ranged from 0.7 to 1.3 mol S/mol N. In our rate experiments, the initial measured S/N ratio was significantly lower than this (0.07–0.12 mol S/mol N).…”

Section: Resultsmentioning

confidence: 99%

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Sulfide alters microbial functional potential in a methane and nitrogen cycling biofilm reactor

Vela

Bristow

Marchant

et al. 2020

Environmental Microbiology

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Cross-feeding of metabolites between coexisting cells leads to complex and interconnected elemental cycling and microbial interactions. These relationships influence overall community function and can be altered by changes in substrate availability. Here, we used isotopic rate measurements and metagenomic sequencing to study how cross-feeding relationships changed in response to stepwise increases of sulfide concentrations in a membraneaerated biofilm reactor that was fed with methane and ammonium. Results showed that sulfide: (i) decreased nitrite oxidation rates but increased ammonia oxidation rates; (ii) changed the denitrifying community and increased nitrous oxide production; and (iii) induced dissimilatory nitrite reduction to ammonium (DNRA). We infer that inhibition of nitrite oxidation resulted in higher nitrite availability for DNRA, anammox, and nitrite-dependent anaerobic methane oxidation. In other words, sulfide likely disrupted microbial cross-feeding between AOB and NOB and induced cross-feeding between AOB and nitrite reducing organisms. Furthermore, these cross-feeding relationships were spatially distributed between biofilm and planktonic phases of the reactor. These results indicate that using sulfide as an electron donor will promote N 2 O and ammonium production, which is generally not desirable in engineered systems.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Sulfide alters microbial functional potential in a methane and nitrogen cycling biofilm reactor

Vela

Bristow

Marchant

et al. 2020

Environmental Microbiology

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“…This may be because short-form S. alterniflora outcompetes S. patens in areas of prolonged flooding partially due to its ability to efficiently oxidize the rooting zone (Mendelssohn et al, 1981), which can increase oxic conditions in the soil that decrease denitrification. Another mechanism for higher potential denitrification in S. alterniflora zones is that prolonged flooding (and lower redox) may also be associated with higher sulfide, which can inhibit heterotrophic denitrification (Yin et al, 2015). Although we did not measure dissolved oxygen concentrations or soil redox as part of our study, high seasonal and diurnal variation in dissolved oxygen concentrations driven by tidal inundation dynamics (Baumann et al, 2015) suggests that vegetation zone may better represent average soil redox conditions than point measurements of dissolved oxygen or other soil water chemistry metrics.…”

Section: Vegetation Zones As Indicators Of Denitrificationmentioning

confidence: 99%

Vegetation zones as indicators of denitrification potential in salt marshes

Ooi

Barry

Lawrence

et al. 2022

Ecological Applications

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Salt marsh vegetation zones shift in response to large-scale environmental changes such as sea-level rise (SLR) and restoration activities, but it is unclear if they are good indicators of soil nitrogen removal. Our goal was to characterize the relationship between denitrification potential and salt marsh vegetation zones in tidally restored and tidally unrestricted coastal marshes, and to use vegetation zones to extrapolate how SLR may influence high marsh denitrification at the landscape scale. We conducted denitrification enzyme activity assays on sediment collected from three vegetation zones expected to shift in distribution due to SLR and tidal flow restoration across 20 salt marshes in Connecticut, USA (n = 60 sampling plots) during the summer of 2017. We found lower denitrification potential in short-form Spartina alterniflora zones (mean, 95% CI: 4, 3-6 mg N h À1 m À2 ) than in S. patens (25, 15-36 mg N h À1 m À2 ) and Phragmites australis (56, 16-96 mg N h À1 m À2 ) zones. Vegetation zone was the single best predictor and explained 52% of the variation in denitrification potential; incorporating restoration status and soil characteristics (soil salinity, moisture, and ammonium) did not improve model fit.Because denitrification potential did not differ between tidally restored and unrestricted marshes, we suggest landscape-scale changes in denitrification after tidal restoration are likely to be associated with shifts in vegetation, rather than differences driven by restoration status. Sea-level-rise-induced hydrologic changes are widely observed to shift high marsh dominated by S. patens to short-form S. alterniflora. To explore the implications of this shift in dominant high marsh vegetation, we paired our measured mean denitrification potential rates with projections of high marsh loss from SLR. We found that, under low and medium SLR scenarios, predicted losses of denitrification potential due to replacement of S. patens by short-form S. alterniflora were substantially larger than losses due to reduced high marsh land area alone. Our results suggest that changes in vegetation zones can serve as

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“…In a microbial community with both DNRA and denitrifying bacteria, DNRA process dominated when glucose and glycerol served as the carbon sources (nitrate removal rate: 2.7-10.1 mgN/g MLVSS/h), while denitrification mainly occurred when acetic acid and lactic acid were supplied (nitrate removal rate: 23.8-27.8 mgN/g MLVSS/h) (Akunna et al, 1993). Both autotrophic denitrifiers and respiratory DNRA bacteria can use inorganic compounds as electron donors (e.g., H 2 , S 2-, Fe 2+ ), but FeS usually enriched denitrifiers, and H 2 S favored the growth of respiratory DNRA bacteria (Yin et al, 2015;Pandey et al, 2020). 2) Environmental factors (e.g., high temperature and pH): Higher diversity and activity of DNRA bacteria were observed in WWTPs operated at higher temperature despite variations in treatment configurations (Wang et al, 2020c).…”

Section: Complete Ammonia Oxidizing (Comammox) Bacteriamentioning

confidence: 99%

Overlooked nitrogen-cycling microorganisms in biological wastewater treatment

Lü

2021

Front. Environ. Sci. Eng.

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Nitrogen-cycling microorganisms play key roles at the intersection of microbiology and wastewater engineering. In addition to the well-studied ammonia oxidizing bacteria, nitrite oxidizing bacteria, heterotrophic denitrifiers, and anammox bacteria, there are some other N-cycling microorganisms that are less abundant but functionally important in wastewater nitrogen removal. These microbes include, but not limited to ammonia oxidizing archaea (AOA), complete ammonia oxidation (comammox) bacteria, dissimilatory nitrate reduction to ammonia (DNRA) bacteria, and nitrate/nitrite-dependent anaerobic methane oxidizing (NOx-DAMO) microorganisms. In the past decade, the development of high-throughput molecular technologies has enabled the detection, quantification, and characterization of these minor populations. The aim of this review is therefore to synthesize the current knowledge on the distribution, ecological niche, and kinetic properties of these “overlooked” N-cycling microbes at wastewater treatment plants. Their potential applications in novel wastewater nitrogen removal processes are also discussed. A comprehensive understanding of these overlooked N-cycling microbes from microbiology, ecology, and engineering perspectives will facilitate the design and operation of more efficient and sustainable biological nitrogen removal processes.

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Effects of sulfide on the integration of denitrification with anaerobic digestion

Cited by 13 publications

References 23 publications

Sulfide alters microbial functional potential in a methane and nitrogen cycling biofilm reactor

Sulfide alters microbial functional potential in a methane and nitrogen cycling biofilm reactor

Vegetation zones as indicators of denitrification potential in salt marshes

Overlooked nitrogen-cycling microorganisms in biological wastewater treatment

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