Effects of sulfated fucan, ascophyllan, from the brown Alga Ascophyllum nodosum on various cell lines: A comparative study on ascophyllan and fucoidan

Abstract

Abstract:The effects of fucose-containing sulfated polysaccharides, ascophyllan and fucoidan, isolated from the brown alga Ascophyllum nodosum, on the growth of various cell lines (MDCK, Vero, PtK 1 , CHO, HeLa, and XC) were investigated. In a colony formation assay, ascophyllan and fucoidan showed potent cytotoxic effects on Vero and XC cells, while other cell lines were relatively resistant to these polysaccharides. Almost no significant effects of these polysaccharides were observed in the cell lines tested using the Alamar blue cytotoxicity assay over 48 h with varying initial cell densities (2500-20,000 cells/well) in growth medium. Interestingly, a significant growth promoting effect of ascophyllan on MDCK cells was observed, whereas treatment with fucoidan showed growth suppressive effects on this cell line under the same experimental conditions. These results suggest that ascophyllan is distinguishable from fucoidan in terms of their bioactivities. This is the first report of the growth promoting effects of a sulfated fucan on a mammalian cell line under normal growth conditions. 4

The involvement of the HO-1 pathway in the anti-inflammatory action of a sulfated polysaccharide isolated from the red seaweed Gracilaria birdiae

“…Marine organisms are sources of structurally diverse bioactive compounds that contain substances with valuable pharmaceutical and biomedical potentials [29,30]. The results reported here demonstrate that SP-Gb exerts an anti- Fig.…”

Biological activities and potential cosmeceutical applications of bioactive components from brown seaweeds: a review

“…It is a sulfated polysaccharide found in the cell-wall matrix of brown seaweeds (Kim et al 2010a;Teruya et al 2007). Among the sulfated polysaccharides, often called fucoidans found in marine brown algae, are well-known to have potent biological activities such as antiinflammatory and antioxidant (Jiang et al 2010). Biological activities of fucoidans seem to be determined by their high degree of sulfation, although, these activities depend on the fine structure and molecular weight (Zvyagintseva et al 2003).…”

Immunostimulatory Activities of the Sulfated Polysaccharide Ascophyllan fromAscophyllum nodosuminin Vivoandin VitroSystems

“…6) Besides several structural and bioactive similarities between fucoidan and ascophyllan, 7,8) our previous study found that ascophyllan showed growth-promoting activity as to MDCK cells, while fucoidan was toxic to this cell line. 9) We also found that ascophyllan induced a much higher level of nitric oxide (NO) production in RAW264.7 cells than those induced by fucoidans isolated from Fucus vesiculosus and Ascophyllum nodosum.…”