Effects of Sucrose, Glucose and Molasses on Fermentation Quality and Bacterial Community of Stylo Silage

Abstract

To better understand the mechanism underlying water-soluble carbohydrates (WSC)-regulated silage fermentation, the bacterial community and fermentation quality of stylo (Stylosanthes guianensis) ensiled without (sterile distilled water) (CK) or with 10 g/kg sucrose (S), 10 g/kg glucose (G), and 10 g/kg molasses (M) were investigated. Each treatment was ensiled in three plastic bag silos for 30 days. The DM of stylo was 301.0 g/kg (fresh matter basis), while the contents of CP, WSC, NDF and ADF were 104.9, 12.3, 510.4 and 407.6 g/kg (dry matter basis), respectively. The bacterial community was characterized by using 16Sr DNA sequencing technology. The addition of WSC significantly altered both fermentation quality and the bacterial community of stylo silage. The additive treatment resulted in higher lactic acid (17.2 vs. 67.0 g/kgDM), lower pH (4.68 vs. 4.46), and lower acetic acid (37.0 vs. 28.9 g/kgDM) compared with the control. In addition, no propionic acid and butyric acid were detected in the additive treatment groups. These results indicated that WSC additives helped to produce greater quality stylo silage. Meanwhile, the Shannon index was higher, and the Simpson index was lower in the WSC additive-treated groups compared with the control, indicating that WSC improved microbial diversity. Furthermore, WSC treatments increased the abundance of acid-producing bacteria Megamonas and Bacteroides, decreased the abundance of Weissella, and inhibited the growth of the undesirable Enterobacter. Our results confirmed that sucrose, glucose and molasses have similar beneficial effects on both bacterial community and silage fermentation of stylo. Molasses was recommended to be used in stylo silage for economic benefit and resource utilization.

Dynamics and correlations of chlorophyll and phytol content with silage bacterial of different growth heights Pennisetum sinese

“…After 60 days ensiling, the bags were opened, 50 g of silage was stored at −80°C for analysis of pigment analysis and 100 g silage was dried at 65°C for 48h for chemical composition analysis ( AOAC, 2000 ). In addition, 50 g silage was blended with 200 ml of distilled water, followed by incubation at 4°C for 24h and then filtration ( Zi et al., 2022 ). Half of each extract sample was stored at −80°C for analysis of the microbiota diversity and the rest of extract sample stored at 4°C for 24h for fermentation quality analysis ( Zi et al., 2022 ).…”

“…In addition, 50 g silage was blended with 200 ml of distilled water, followed by incubation at 4°C for 24h and then filtration ( Zi et al., 2022 ). Half of each extract sample was stored at −80°C for analysis of the microbiota diversity and the rest of extract sample stored at 4°C for 24h for fermentation quality analysis ( Zi et al., 2022 ).…”

“…The P. sinese silage microbial (genus) and pigments and phytol content were used R software to generate a heat map. Above methods details were as previously described (Zi et al, 2021;Zi et al, 2022). The data were analyzed using the free online BMKCloud Platform (www.biocloud.net).…”

“…Phytol is produced by chlorophyll degradation in acidic environments, and the acidity of silage may be the key factor of phytol conversion. During the ensiling, pH value was mainly affected by the silage microbial community ( Du et al., 2022 ; Zi et al., 2022 ). Therefore, the resolving of silage microbial community structure may help to improve the efficiency of phytol conversion.…”

Current approaches on the roles of lactic acid bacteria in crop silage

“…Nonetheless, glucogenic 1,2-propanediol is used in veterinary practice for the treatment of clinical ketosis (Zielińska et al, 2017). Upon consumption by dairy cows, 1,2-propanediol could be absorbed and converted to glucose in the liver or converted to propionic acid in the rumen (Kung et al, 2018).…”