Effects of sucrose, caffeine, and cola beverages on obesity, cold resistance, and adipose tissue cellularity

Bukowiecki, L. J.; Lupien, J.; Follea, N.; Jahjah, Liliane

doi:10.1152/ajpregu.1983.244.4.r500

Cited by 37 publications

(30 citation statements)

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“…The consumption of caffeine including coffee, tea and caffeine-rich food was not allowed for at least 2 weeks before the experiment and no sports activities Caffeine is a naturally consumed substance contained in beverages such as coffee, tea, cocoa, and some cola drinks (Bukowiecki et al 1983). It is well established that caffeine increases the human metabolic rate (Yoshida et al 1995).…”

Section: Methodsmentioning

confidence: 99%

Lack of Association of the Trp64Arg Polymorphism of .BETA.3-Adrenergic Receptor Gene with Energy Expenditure in Response to Caffeine Among Young Healthy Women

Hamada

Kotani

Higashi

et al. 2008

Tohoku J. Exp. Med.

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A close relationship between coffee intake and certain metabolic disorders is known. Caffeine, one of coffee components, can increase energy expenditure (EE), but there are considerable individual differences in the caffeine effects on EE, and the causes have not been fully established in humans. The Arg allele in the β 3 -adrenergic receptor gene (β 3 -AR), a marker for obesity-related traits, may be a contributor to individual variations in EE. This study investigated the effect of the Arg allele of β 3 -AR on caffeine-induced increases in EE. In 44 healthy young women (21 ± 1 years), physical characteristics, blood pressure, biochemical profiles and dietary nutritional intake were measured. A caffeineloading test was conducted at a dosage of 4 mg per body weight (kg). EE was measured using an indirect open-circuit calorimeter for a 10-min period before, and at 30 min and 60 min after the caffeine-loading test. The β 3 -AR Trp64Arg polymorphism was detected with a PCR-restriction fragment length polymorphism method. The frequency of the Arg allele was 24%. The distribution of the Trp/Trp, Trp/Arg, and Arg/Arg genotypes was 58%, 36%, and 6%, respectively. At the baseline, subjects with the Arg/Arg genotype had a significantly lower EE level than those with the Trp/Trp or Trp/Arg genotype. After the caffeine-loading test, there were caffeine-induced increases in EE in all genotypes, but there were no differences in the levels of increase among the genotypes. These findings suggest that the genotypes of β 3 -AR Trp64Arg polymorphism might be not associated with caffeine-induced increases in EE levels.β 3 -adrenergic receptor; polymorphism; caffeine; energy expenditure; genotype.Tohoku

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Section: Methodsmentioning

confidence: 99%

Lack of Association of the Trp64Arg Polymorphism of .BETA.3-Adrenergic Receptor Gene with Energy Expenditure in Response to Caffeine Among Young Healthy Women

Hamada

Kotani

Higashi

et al. 2008

Tohoku J. Exp. Med.

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“…Several studies have reported that caffeine increases lipolysis and energy expenditure (13,14) and inhibits hypertrophy of mature adipocytes via suppression of glucose intake (15). Furthermore, it is reported that the intake of a diet containing caffeine reduced the weight of parametrical white adipose tissues in rats (16), and the weight of intraperitoneal adipose tissues in mice (17). To examined the effect of epithelial cells stimulated by caffeine on lipid accumulation in adipocytes, caffeine was added to the apical side of Caco-2 monolayers in a transwell, and 3T3-L1 cells were treated with the permeated basolateral medium, termed caffeine-conditioning medium (CCM).…”

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confidence: 99%

Caffeine-Stimulated Intestinal Epithelial Cells Suppress Lipid Accumulation in Adipocytes

Mitani

Nagano

Harada

et al. 2017

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Summary Caffeine is a methylxanthine derived from plant foods such as coffee beans and tea leaves, and has multiple biological activities against physiological response and several diseases. Although there are some reports about the direct effect of caffeine against anti-lipid accumulation in vitro, the effect of caffeine on lipid accumulation in adipocytes through stimulating intestinal epithelial cells is unknown. Since direct treatment with caffeine to 3T3-L1 cells did not affect lipid accumulation, we determined whether caffeinestimulated intestinal epithelial Caco-2 cells influence the lipid accumulation in 3T3-L1 adipocytes. Caco-2 cells were cultured on a transwell insert with or without caffeine for 24 h. Subsequently, the basolateral component of the Caco-2 cell culture on the transwell was collected and termed caffeine-conditioning medium (CCM). When 3T3-L1 adipocytes were incubated with CCM, CCM decreased lipid accumulation and suppressed gene expression of proliferator activated receptor (PPAR) ␥ and CCAAT/enhancer binding protein (C/EBP) ␣ in 3T3-L1 adipocytes. Furthermore, CCM decreased the expression of C/EBP␤ and C/EBP␦ at the protein level, but not at the mRNA level. We observed that a proteasome inhibitor, MG132, inhibited CCM-caused down-expression of C/EBP␤ and C/EBP␦ proteins, and that CCM promoted the ubiquitination level of C/EBP␤ and C/EBP␦ proteins. Protein microarray analysis showed caffeine suppresses the secretion of inflammatory cytokines, interleukin-8 and plasminogen activator inhibitor-1 from Caco-2 cells. These results suggest that caffeine indirectly suppresses lipid accumulation in 3T3-L1 adipocytes through decreasing secretion of inflammatory cytokines from Caco-2 cells. Key Words caffeine, adipogenesis, 3T3-L1 adipocytes, Caco-2 cells Obesity is a risk factor for multiple lifestyle diseases such as apoplexy, heart disease and diabetes (1, 2). Adipocytes play a critical role in regulating lipid metabolism and energy balance, which are associated with adiposity (3). An increase in the number of differentiated mature adipocytes and excess accumulation of body fat leads to adiposity. Adipocytes accumulate intracellular lipids during differentiation. As an in vitro model system for adipogenesis, murine 3T3-L1 cells, which were originally derived from mouse embryos, are often used. Differentiation of 3T3-L1 cells is induced by exposure to the inducers, such as 3-isobutyl-1-methylxanthine (IBMX), dexamethasone and insulin, and is responsible for the accumulation of a large amount of intracellular lipid droplets during adipocyte differentiation. Adipogenesis, the differentiation process for producing adipocytes, is a complex process that is regulated by various transcription factors and their related genes. The expression levels of CCAAT/enhancer binding protein (C/EBP) ␤ and C/EBP␦, as transcriptional factors, are increased in the early phase of adipocyte differentiation (4). Subsequently, these transcription factors up-regulate proliferator activated receptor (PPAR) ␥ and C/EBP␣, a...

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“…2) CF has also been demonstrated to reduce the weight of adipose tissues in experimental animals fed with a normal diet. For instance, Bukowiecki et al 3) have reported that the intake of 0.057% and 0.2% of CF for 9 weeks both reduced the weight of parametrial white adipose tissues in rats. Chen et al 4) have shown that a CF intake (4 mg/ day) for 4 weeks decreased the weight of body fat in genetically obese mice, and Michna et al 5) have reported that the ingestion of 0.04% CF for 15 weeks decreased the weight of the parametrial fat pad by 43% in mice.…”

mentioning

confidence: 99%

“…These results show that the CF intake suppressed the accumulation of body fat. There have been several reports on the antiobesity action of CF alone under the condition of a normal diet by Zheng et al, 6) who showed that a 0.05% CF intake reduced the weight of intraperitoneal adipose tissues in mice, and by Bukowiecki et al, 3) who reported that 0.057% CF decreased the weight and triglyceride content of parametrial white adipose tissues in rats. The results of the present study show that even 0.025% CF, which corresponds to the daily intake of CF that is contained in tea and coffee, had an anti-obesity effect under the influence of a high-fat diet.…”

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confidence: 99%

Effect of Caffeine on the Body Fat and Lipid Metabolism of Rats Fed on a High-Fat Diet

Kobayashi‐Hattori

Mogi

Matsumoto

et al. 2005

Bioscience, Biotechnology, and Biochemistry

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The intake of caffeine (CF) at 0.025, 0.05 or 0.1% for 21 days progressively reduced the body fat mass and body fat percentage in Sprague-Dawley (SD) rats fed on a high-fat diet with increasing administration level. Moreover, CF increased the serum concentrations of catecholamines and free fatty acids in SD rats orally administered with CF (5 mg/kg). These results suggest that the intake of CF reduced body fat by lipolysis via catecholamines. CF has potential as a functional food ingredient with an anti-obesity action.

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Effects of sucrose, caffeine, and cola beverages on obesity, cold resistance, and adipose tissue cellularity

Cited by 37 publications

References 0 publications

Lack of Association of the Trp64Arg Polymorphism of .BETA.3-Adrenergic Receptor Gene with Energy Expenditure in Response to Caffeine Among Young Healthy Women

Lack of Association of the Trp64Arg Polymorphism of .BETA.3-Adrenergic Receptor Gene with Energy Expenditure in Response to Caffeine Among Young Healthy Women

Caffeine-Stimulated Intestinal Epithelial Cells Suppress Lipid Accumulation in Adipocytes

Effect of Caffeine on the Body Fat and Lipid Metabolism of Rats Fed on a High-Fat Diet

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