Effects of Subacute and Subchronic Treatment of Synthetic Plant Growth Regulators on Liver Damage Serum Biomarkers Tissue Antioxidant Defense Systems and Lipid Peroxidation in Rats

Abstract

The present study was aimed to investigate the effects of some synthetic plant growth regulators; ß-Naphthoxy acetic acid (ß-NOA) and 4-Chlorophenoxy acetic acid (4-CPA) at 10 and 20 ppm dosages on liver damage, antioxidant defense system and lipid peroxidation in rats. Rats were divided into 12 groups as control, ß-NOA and 4-CPA at 10 and 20 ppm dosages application groups. Serum marker enzymes and biochemical parameters were selected as biomarkers for liver damage. Antioxidant defense systems as SOD, GR, CAT, GSH-Px, GST and G6PD enzyme activities and GSH and MDA contents were determined in tissues as the indicators of oxidative stress. At the end of experiments, the statistically significant changes were observed in the levels of TPRO, TALB, TCHOL, TBIL, AST, ALT and LDH as liver damage serum biomarkers in rats exposed to 10 and 20 ppm of ß-NOA and 4-CPA as compared with controls. MDA levels increased in all the tissues of rats exposed to ß-NOA and 4-CPA dosages whereas the fluctuation in SOD, GR, CAT, GSH-Px, GST and G6PD enzyme activities and GSH levels were observed compared with controls. It could be concluded that the treatments of ß-NOA and 4-CPA dosages may induce liver damage and oxidative stress.

Anti-hyperglycemic effect of Vicia ervilia (L.) Willd (Fabaceae) seed extract and its effect on lipid profile, and hepatic and renal biomarkers in streptozotocin-induced diabetic rats

“…The tissues were homogenized in ultrasonic homogenizer for 5 minutes (Jencons Scientific Co., Bedfordshire, UK, 20 kHz frequency ultrasonic,). The homogenates were centrifuged in a cooled centrifuge device (Hettich Universal 320 R) at 9500 rpm for 30 min at 4 o C. Alphaglucosidase enzyme activity was measured in the supernatants obtained from small intestine tissues [13].…”

Assessment toxic effects of exposure to 3-indoleacetic acid via hemato-biochemical, hormonal, and histopathological screening in rats

“…Although some experiments were performed on animals or cell cultures in vitro for assessment of the PGRs' harmful impacts on animal and human (Folkes et al 1999;Celik et al 2006a), knowledge regarding the toxic effects of PGRs on mammalian organisms is limited or incomplete (Ozok and Celik 2012). Previous studies showed some effects of IAA on biological systems, such as inducing severe cytotoxicity via oxidative stress on lipid membranes and nucleic acids and increasing ROS generation (Folkes and Wardman 2001;de Melo et al 2004).…”