Effects of structural analogs of brassinosteroids on the recovery of growth inhibition by a specific brassinosteroid biosynthesis inhibitor

Mazorra, L. M.; Núñez, Miriam; Nápoles, María C.; Robaina, Caridad; Coll, Francisco; Asami, Tadao

doi:10.1023/b:grow.0000049412.30708.d3

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…Referring to the land plants, BL reversed the dwarf phenotype of A. thaliana seedling treated with Brz [ 20 ]. While, in plants treated with Brz2001, the recovery effects of treatment with EBL on the length of soybean seedling [ 65 ] and algal growth (expressed as a number of cells) [ 26 ] were also observed.…”

Section: Discussionmentioning

confidence: 99%

Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole

Chmur

Bajguz

2021

Plants

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Brassinolide (BL) represents brassinosteroids (BRs)—a group of phytohormones that are essential for plant growth and development. Brassinazole (Brz) is as a synthetic inhibitor of BRs’ biosynthesis. In the present study, the responses of Wolffia arrhiza to the treatment with BL, Brz, and the combination of BL with Brz were analyzed. The analysis of BRs and Brz was performed using LC-MS/MS. The photosynthetic pigments (chlorophylls, carotenes, and xanthophylls) levels were determined using HPLC, but protein and monosaccharides level using spectrophotometric methods. The obtained results indicated that BL and Brz influence W. arrhiza cultures in a concentration-dependent manner. The most stimulatory effects on the growth, level of BRs (BL, 24-epibrassinolide, 28-homobrassinolide, 28-norbrassinolide, catasterone, castasterone, 24-epicastasterone, typhasterol, and 6-deoxytyphasterol), and the content of pigments, protein, and monosaccharides, were observed in plants treated with 0.1 µM BL. Whereas the application of 1 µM and 10 µM Brz caused a significant decrease in duckweed weight and level of targeted compounds. Application of BL caused the mitigation of the Brz inhibitory effect and enhanced the BR level in duckweed treated with Brz. The level of BRs was reported for the first time in duckweed treated with BL and/or Brz.

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Section: Discussionmentioning

confidence: 99%

Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole

Chmur

Bajguz

2021

Plants

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“…However, the inhibition could be reversed by the exogenous treatment with EBL. Similarly, it was also reported that Brz induced dwarfism and curly phenotypes in soybean (Glycine max) (Mazorra et al 2004), dark-green leaves in light-grown cress (Lepidium sativum), cucumber (Cucumis sativus), tobacco (Nicotiana tabacum) (Nagata et al 2001;Sekimata et al 2001) and inhibition of Wolffia arrhiza growth (Bajguz and Asami 2005).…”

Section: Discussionmentioning

confidence: 71%

Occurrence of brassinosteroids and influence of 24-epibrassinolide with brassinazole on their content in the leaves and roots of Hordeum vulgare L. cv. Golden Promise

Bajguz

Orczyk

Gołębiewska

et al. 2018

Planta

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Main conclusion 24-epibrassinolide overcame the inhibitory effect of brassinazole on the barley growth and the content of brassinosteroids. The present work demonstrates the occurrence of mainly castasterone, brassinolide and cathasterone and lower amounts of 24-epibrassinolide, 24-epicastasterone, 28-homobrassinolide, typhasterol, 6-deoxocastasterone and 6-deoxotyphasterol in 14-day-old de-etiolated barley (Hordeum vulgare L. cv. Golden Promise). We also investigated the endogenous level of brassinosteroids (BRs) in barley seedlings treated with 24-epibrassinolide (EBL) and/or brassinazole (Brz). To our knowledge, this is the first report related to the occurrence of BRs and application of EBL and Brz in terms of the endogenous content of BRs in barley. Brz as a specific inhibitor of BR biosynthetic reactions decreased the level of BRs in the leaves. Application of EBL showed a weak promotive effect on the BR content in Brz-treated seedlings. Brz also inhibited growth of the seedlings; however, addition of EBL overcame the inhibition. The EBL applied alone at 0.01-1 µM increased the BR level in the leaves but at 10 µM lowered the BR content. In opposition to leaves, the Brz in the concentration range from 0.1 to 1 µM did not significantly affect the content of BRs in the roots. However, application of 10 µM Brz caused BRs to decrease, but treatment of EBL concentrations overcame the inhibitory effect of Brz.

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Brassinosteroid and brassinosteroid-mimic differentially modulate Arabidopsis thaliana fitness under drought

et al. 2021

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Effects of structural analogs of brassinosteroids on the recovery of growth inhibition by a specific brassinosteroid biosynthesis inhibitor

Cited by 3 publications

References 7 publications

Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole

Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole

Occurrence of brassinosteroids and influence of 24-epibrassinolide with brassinazole on their content in the leaves and roots of Hordeum vulgare L. cv. Golden Promise

Brassinosteroid and brassinosteroid-mimic differentially modulate Arabidopsis thaliana fitness under drought

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