Effects of streptovitacin A on the initial events in the replication of vaccinia and reovirus.

Dales, Samuel

doi:10.1073/pnas.54.2.462

Cited by 37 publications

(14 citation statements)

References 11 publications

(1 reference statement)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Biochemical and electron-micrographic studies have shown the virion to undergo at least two discrete stages of disassembly on entry into the cell. The first stage is associated with the loss of virion proteins and lipid (108), although the genome is still sequestered in the core, as indicated by its resistance to attack by exogenously added DNase. During the second stage, the genome becomes accessible to DNase (215,415).…”

Section: Uncoatingmentioning

confidence: 99%

Poxvirus pathogenesis.

Buller

Palumbo

1991

Microbiological Reviews

293

163

View full text Add to dashboard Cite

Marchal (285) and Downie (126) bodies, respectively. Because of the ambiguity associated with describing structures solely on the basis of their reaction with various histochemical stains, Kato et al. (253) carefully examined the virusspecific cytoplasmic structures of a large number of poxvirus species and, using a criterion based on morphologic observations, histochemical staining, and immunochemical reactivity, suggested that the acidophilic and basophilic inclusion bodies be referred to as A-and B-type inclusion bodies, respectively. Under the auspices of a Poxvirus Subcommittee created at the Sixth International Congress for Microbiology in 1953, Fenner and Burnet (161) wrote a review which summarized the characteristics of the poxvirus group. This article remains the basis for the subsequent classification of poxviruses. The poxvirus family is divided into two subfamilies: Entomopoxvirinae (poxviruses of the insects) and Chordopoxvirinae (poxviruses of the vertebrates); the latter group of viruses is the focus of this review (Table 1). The vertebrate poxviruses share a group-specific NP antigen (511) and are able, in a reciprocal fashion, to participate in nongenetic reactivation (160, 168). This is a process whereby an infectious poxvirus is able to reactivate a second, heatinactivated poxvirus, as indicated by the production of progeny virus. Poxviruses were further classified into genera by comparing cross-protection in animal studies, crossneutralization of infectivity in tissue culture, and crosshybridization of genomic DNA from virions. This latter criterion is now the method of choice for preliminary classification of poxvirus isolates. In general, members of the same genera have similar morphology and biological properties. Virion Morphology Poxviruses are the largest of all animal viruses and can be visualized by light microscopy, although the details of the

show abstract

Section: Uncoatingmentioning

confidence: 99%

Poxvirus pathogenesis.

Buller

Palumbo

1991

Microbiological Reviews

293

163

View full text Add to dashboard Cite

show abstract

“…For second-stage uncoating to occur, there is an absolute requirement for both RNA and protein synthesis. This point is very secure, and it has been demonstrated many times by the use of inhibitors which result in the accumulation of cores (10,25,34). The result suggests that information encoded in the base sequence of DNA is required for liberation of the viral DNA from the core.…”

Section: Uncoating Of the Viral Nucleic Acidmentioning

confidence: 96%

“…The requirement for protein synthesis is indeed real. Inhibitors such as cycloheximide and streptovitacin inhibit second-stage uncoating completely (10,25,34). Although no good evidence has been obtained to show that a specific protein is required, this has generally been assumed to be the case.…”

Section: Uncoating Of the Viral Nucleic Acidmentioning

confidence: 99%

Recent progress in poxvirus research.

Woodson¹

1968

Bacteriological Reviews

View full text Add to dashboard Cite

“…Specifically, the inhibitory effect of acetone in the rabbitpox virus-chick embryo fibroblast system is as manifest as that in the Lcell system, but there are certain differences in the mode of inhibition (in preparation). The mechanisms of action of inhibitors of replication of pox viruses that have been studied so far involve either the inhibition of some virusspecific synthetic processes (3,10,16,24) or the impairment of virion assembly (5, 9, 13-15, 17, 21). Formation of noninfectious virions by a ts mutant of vacciia virus has also been reported, but the defect was considered to involve an early function on the basis of temperature-shift experiments (1).…”

Section: 'Acetone Particles"mentioning

confidence: 99%

Mechanism of Antiviral Action of Acetone on Rabbitpox Virus Replication

Chernos¹,

Libshits²,

Yakobson³

et al. 1972

J Virol

View full text Add to dashboard Cite

Acetone added to the maintenance medium in a 1% concentration reduces the yield of infectious rabbitpox virus in L-cell monolayer cultures by 90 to 97%. This concentration of the inhibitor is not toxic for cells. It was established that there is an inhibitor-sensitive stage late in the infectious cycle. Acetone exerted no significant influence on production of early viral messenger ribonucleic acid, formation of polyribosomes, deoxyribonucleic acid (DNA) replication, or protein synthesis. In the presence of acetone, the assembly of so-called "acetone particles" occurred. These particles are similar to normal virions by morphological and sedimentation properties but are slightly different from them in buoyant density. The amount of virusspecific DNA and the optical density of the "acetone particles" are the same as those of normal virions despite a 10to 25-fold difference in the infectivity of the preparations.It has been shown previously (4) that acetone added to the maintenance medium in a final concentration of 1% significantly inhibits replication of poxviruses. The possibility of the selection of mutants resistant to the inhibitor has been demonstrated (4). The present work was devoted to the study of the mechanism of action of this inhibitor. MATERLIALS AND METHODSCells. An L-cell monolayer culture was grown in medium 199 supplemented with 10% bovine serum and antibiotics.Virus. Rabbitpox virus, Utrecht strain, was propagated in chick embryos, concentrated, and purified by Joklik's method (7). Virus titration was performed on chorioallantoic membranes of 12-dayold chick embryos according to Westwood (23), with the use of five embryos per dilution. The infectious titer of the virus used in the experiments was 1.5 X 1010 to 2 X 1010 plaque-forming units (PFU)/ml. Conditions of cell inoculation and maintenance of in-fected monolayers. Generally, the multiplicity of infection was 30 to 50 PFU/cell. In the case of the experiments on polyribosome analysis and determination of protein synthesis, the multiplicity of infection was 200 to 300 PFU/cell. The virus was sonically treated before inoculation. The virus was allowed to adsorb on the cells at 36 C for 20 min. The unadsorbed virus was removed, and the cells were washed with warm Earle's saline. The time postinfection was counted from the moment of the addition of warm medium 199. At certain intervals postinfection, the cells were disrupted by three cycles of freezing and thawing, and the virus yields were determined.Acetone. In all experiments, unless indicated other-wise, acetone (to a final concentration of 1%, v/v) was added together with medium 199 without serum immediately after adsorption.Preparationof thecytoplasmic extract. The cells were washed with cold Earle's saline, scraped off with a rubber policeman, and sedimented by centrifugation at 1,000 X g for 15 min. Then the cells were resuspended in hypotonic buffer. In the experiments on polyribosomes, the following buffer was used: 0.01 M triethanolamine-HCl, pH 7.8, 0.01 M NaCl, and 0.003 M Mg?+ (refe...

show abstract

Effects of streptovitacin A on the initial events in the replication of vaccinia and reovirus.

Cited by 37 publications

References 11 publications

Poxvirus pathogenesis.

Poxvirus pathogenesis.

Recent progress in poxvirus research.

Mechanism of Antiviral Action of Acetone on Rabbitpox Virus Replication

Contact Info

Product

Resources

About