Effects of storage temperature on hematopoietic stability and microbial safety of BM aspirates

Abstract

Bone Marrow (BM) remains a common source for hematopoietic SCT. Due to the transcutaneous approach, contamination with skin bacteria is common. The delay between harvest and transfusion can be considerable, potentially allowing for bacterial proliferation. The optimal transportation temperature, specifically with respect to bacterial growth and consequences thereof for hematopoietic quality, remain undefined. For 72 h, 66 individual BM samples, non-spiked/spiked with different bacteria, stored at 20-24 1C room temperature (RT) or 3-5 1C (cold), were serially analyzed for hematopoietic quality and microbial burden. Under most conditions, hematopoietic quality of BM was equal or better at RT: Typical BM contaminants (P. acnes and S. epidermidis) and E. coli were killed or bacterial proliferation was arrested at RT; hematopoietic quality was not impacted by the contamination. However, several pathogenic bacteria not typically found in BM (S. aureus and K. pneumoniae) proliferated dramatically at RT and impaired hematopoietic quality. Bacterial proliferation was arrested in the cold. The overwhelming majority of BM samples, that is, those that are sterile or contaminated only with skin commensals, will benefit from transportation at RT. Those bacteria that proliferate and perturb hematopoietic quality are not typically found in BM. Our data support recommendations for RT transportation and storage of BM.

BAM15 attenuates transportation-induced apoptosis in iPS-differentiated retinal tissue

“…As to the temperature regulation, low temperature weakened temperature-dependent chemical bonds in cytoskeletal support, thus leading the cell more vulnerable to mechanical damage [6]. It also indicated that the majority of fresh blood products and sterile BM samples benefitted from transportation at room temperature [7,8]. Similarly, low temperature is not favorable for shipment of retinal tissues in our study.…”

“…Important considerations related to transportation include density of tissues, shipment duration, temperature management, and transportation medium [6]. Since the former three considerations have been investigated previously on other organs or stem cells [5,[7][8][9][10], we focused on seeking appropriate anti-apoptotic medium for the first time on transporting hiPSC-differentiated retinal tissues.…”

Automated CD34+ cell isolation of peripheral blood stem cell apheresis product

“…As is apparent from Figure 1, Prodigy only minimally reduces process time over CliniMACS Plus, but the hands-on operator time is reduced by almost 2 h. Because the Prodigy process could run unsupervised or supervised remotely overnight, a possible advantage would be avoidance of unfavorable working hours while recovering cells of maximal freshness and, hence, functional quality, given that experience shows approximately linear cell loss over time, starting from the moment that the cells are collected from the donor [21]. The process of CD34 selection is maximally simplified by Prodigy.…”

Staphylococcus aureusBloodstream Infection Due to Contaminated Hematopoietic Stem-Cell Graft

“…However, pathogenic bacteria such as E. coli and S. aureus are capable of rapid expansion within HSC products stored at room temperature. 9 Certain organisms, such as Candida spp and S. aureus, are particularly capable of forming biofilms on foreign material. Contamination with highly virulent organisms, while rare, may expose HCT recipients to much greater risk than contamination with less pathogenic organisms.…”