“…The dark-adapted dry leaves were extracted immediately after dried (70°C) for 48 h, with acetone (80%) in a pestle with quartz. As presented by Mathura et al (2006), the dried samples could be used for pigment analysis, as the dried leaves that were stored for seven days resulted in the least amount of chlorophyll degradation followed by 28 days ice storage, seven day ice storage and lastly 28 days dried storage. The absorbance was determined with a recording spectrophotometer (U3200, Hitachi, Ibaraki, Japan).…”