Effects of Steroid Hormones on Gene Regulation and Cell Metabolism

Hechter, Oscar; Halkerston, I. D. K.

doi:10.1146/annurev.ph.27.030165.001025

Cited by 70 publications

(10 citation statements)

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“…At 3 and 6 h all tissues showed grain densities that did not differ significantly from control values. Luminal epithelium 8-6 + 0-7 10-9 + 0-6 8-2 + 0-5 7-5 + 0-5 Glandular epithelium 12-0 + 0-7 15-4 + 0-8 11-4 + 0-9 11-5 + 0-8 Stroma 5-6±0-6 9-3 + 0-7 4-9 + 0-4 7-1 + 0-5 Muscle 6-6 + 0-6 11-1 + 0-7 8-2 + 0-6 7-8 + 0-5 DISCUSSION Experiments with metabolic inhibitors Several explanations must be considered for any effect of actinomycin D or cyclo¬ heximide on hormone action (Hechter & Halkerston, 1965). The known toxicity of these drugs may produce effects from non-specific causes such as metabolic dis¬ turbance or reduction in food intake.…”

Section: Autoradiography After [3h]lysine Administrationmentioning

confidence: 99%

Analysis of the Effects of Progesterone on the Synthesis of Rna and Protein in the Uterus of the Ovariectomized Rat and on the Development of an Iodide Concentrating Mechanism

Brown-Grant¹,

John²,

Rogers³

1972

Journal of Endocrinology

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SUMMARY Concentration of radioiodide in the cells of the luminal epithelium of the ovariectomized rat after a single injection of progesterone was abolished by 300 μg cycloheximide/100 g body weight given with or 4 h after the progesterone. Actinomycin D at 32 or 80 μ/100 g body weight given with the progesterone did not inhibit the development of the iodide concentrating mechanism. Oestradiol given 16 h before or 2 h after progesterone inhibited the iodide response in a dose-dependent manner. The incorporation of [3H]uridine into histologically fixed material was significantly increased in all tissues of the uterus 1 h after progesterone. The incorporation of [3H]lysine into histologically fixed material was increased significantly in luminal epithelium at 3 h and in all uterine tissues at 7 and 24 h. These findings are consistent with the view that gene transcription may be stimulated by progesterone, resulting in the synthesis of proteins in the different tissues of the uterus. In the luminal epithelium however, progesterone may also stimulate protein synthesis and the development of the iodide response in the absence of gene transcription.

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Section: Autoradiography After [3h]lysine Administrationmentioning

confidence: 99%

Analysis of the Effects of Progesterone on the Synthesis of Rna and Protein in the Uterus of the Ovariectomized Rat and on the Development of an Iodide Concentrating Mechanism

Brown-Grant¹,

John²,

Rogers³

1972

Journal of Endocrinology

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“…land and Harper, 1957;Hechter and Halkerston, 1965;Potter and Ono, 1961 ;Smith and Haijer, 1962;Weber, 1963). In no case, however, has the precise mechanism of control been established.…”

Section: Studies On the Mechanisms Underlying Adaptive Changes Inmentioning

confidence: 99%

Studies on the Mechanisms Underlying Adaptive Changes in Rat Liver Phosphoenolpyruvate Carboxykinase^*

Foster¹,

Ray²,

Lardy³

1966

Biochemistry

110

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The regulation of hepatic phosphoenolpyruvate carboxykinase activity in vivo was studied by measuring the activity of the enzyme in the soluble fraction of livers obtained from rats subjected to various alterations in their hormonal or metabolic status. Puromycin and actinomycin D were employed in some experiments to inhibit, respectively, protein and ribonucleic acid synthesis. The data indicate that the tissue activity of the enzyme is regulated not only by changes in the rate of biosynthesis of the enzyme, but also in one or more other ways which may involve activation processes or changes in the rate of degradatipn of the T he adaptation of many of the enzymic activities of mammalian tissues in response to alterations in the endocrine, nutritional, or environmental status of animals has been intensively studied in recent years (Freed-

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“…Since the hormones released during a stress response impact metabolic pathways [32], [33] investigations of whole-animal metabolism may be particularly sensitive to artifacts of stress responses. For example, acute stress responses result in increased levels of glucocorticoids and catecholamines, which affect the release of glucose into plasma [34].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Handling and Anesthetic Agents on the Stress Response and Carbohydrate Metabolism in Northern Elephant Seals

et al. 2012

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Free-ranging animals often cope with fluctuating environmental conditions such as weather, food availability, predation risk, the requirements of breeding, and the influence of anthropogenic factors. Consequently, researchers are increasingly measuring stress markers, especially glucocorticoids, to understand stress, disturbance, and population health. Studying free-ranging animals, however, comes with numerous difficulties posed by environmental conditions and the particular characteristics of study species. Performing measurements under either physical restraint or chemical sedation may affect the physiological variable under investigation and lead to values that may not reflect the standard functional state of the animal. This study measured the stress response resulting from different handling conditions in northern elephant seals and any ensuing influences on carbohydrate metabolism. Endogenous glucose production (EGP) was measured using [6-3H]glucose and plasma cortisol concentration was measured from blood samples drawn during three-hour measurement intervals. These measurements were conducted in weanlings and yearlings with and without the use of chemical sedatives—under chemical sedation, physical restraint, or unrestrained. We compared these findings with measurements in adult seals sedated in the field. The method of handling had a significant influence on the stress response and carbohydrate metabolism. Physically restrained weanlings and yearlings transported to the lab had increased concentrations of circulating cortisol (F11, 46 = 25.2, p<0.01) and epinephrine (F3, 12 = 5.8, p = 0.01). Physical restraint led to increased EGP (t = 3.1, p = 0.04) and elevated plasma glucose levels (t = 8.2, p<0.01). Animals chemically sedated in the field typically did not exhibit a cortisol stress response. The combination of anesthetic agents (Telazol, ketamine, and diazepam) used in this study appeared to alleviate a cortisol stress response due to handling in the field without altering carbohydrate metabolism. Measures of hormone concentrations and metabolism made under these conditions are more likely to reflect basal values.

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Effects of Steroid Hormones on Gene Regulation and Cell Metabolism

Cited by 70 publications

References 1 publication

Analysis of the Effects of Progesterone on the Synthesis of Rna and Protein in the Uterus of the Ovariectomized Rat and on the Development of an Iodide Concentrating Mechanism

Analysis of the Effects of Progesterone on the Synthesis of Rna and Protein in the Uterus of the Ovariectomized Rat and on the Development of an Iodide Concentrating Mechanism

Studies on the Mechanisms Underlying Adaptive Changes in Rat Liver Phosphoenolpyruvate Carboxykinase^*

The Effects of Handling and Anesthetic Agents on the Stress Response and Carbohydrate Metabolism in Northern Elephant Seals

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Effects of Steroid Hormones on Gene Regulation and Cell Metabolism

Cited by 70 publications

References 1 publication

Analysis of the Effects of Progesterone on the Synthesis of Rna and Protein in the Uterus of the Ovariectomized Rat and on the Development of an Iodide Concentrating Mechanism

Analysis of the Effects of Progesterone on the Synthesis of Rna and Protein in the Uterus of the Ovariectomized Rat and on the Development of an Iodide Concentrating Mechanism

Studies on the Mechanisms Underlying Adaptive Changes in Rat Liver Phosphoenolpyruvate Carboxykinase*

The Effects of Handling and Anesthetic Agents on the Stress Response and Carbohydrate Metabolism in Northern Elephant Seals

Contact Info

Product

Resources

About

Studies on the Mechanisms Underlying Adaptive Changes in Rat Liver Phosphoenolpyruvate Carboxykinase^*