Effects of sterilization methods and plant growth regulators on in vitro regeneration and tuberization in Gloriosa superba (L.)

Mosoh, Dexter Achu; Khandel, Ashok Kumar; Verma, Sandeep Kumar; Vendrame, Wagner A.

doi:10.1007/s11627-023-10387-9

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Cited by 5 publications

References 41 publications

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Phytochemical analysis and enhanced production of alkaloids in non-dormant corm-derived callus of Gloriosa superba (L.) using plant growth regulators and abiotic elicitors

Mosoh,

Khandel,

Verma

et al. 2024

Plant Cell Tiss Organ Cult

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Phytochemical analysis and enhanced production of alkaloids in non-dormant corm-derived callus of Gloriosa superba (L.) using plant growth regulators and abiotic elicitors

Mosoh,

Khandel,

Verma

et al. 2024

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Development of efficient, cost-effective in vitro micropropagation technique for threatened ethnomedicinal plant Clerodendrum indicum (L.) O. Kuntze

Kundu,

Sahani,

Adhikary

et al. 2024

Plant Cell Tiss Organ Cult

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Development of efficient, cost-effective in vitro micropropagation technique for threatened ethnomedicinal plant Clerodendrum indicum (L.) O. Kuntze

Kundu,

Adhikary,

Sahani

et al. 2023

Preprint

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Clerodendrum indicum (L.) O. Kuntze (Verbenaceae) is a threatened ethnomedicinal plant with many bioactive secondary metabolites that could alleviate chronic diseases like cough, asthma, jaundice, leprosy, syphilitic rheumatism, and septic wounds. Their natural growth has been severely challenged due to habitat loss and massive exploitation for medical applications, leading to the threatened status. Therefore, an in vitro micropropagation technique has been trialled to be used for eco-restoration and metabolite exploitation. Micropropagation via direct and indirect shoot organogenesis had been established from the different explants of this plant. Murashige and Skoog (MS) media supplemented with variable concentrations of NAA, IAA, and BAP produced callus, organogenesis and whole plant. The study revealed that nodal explants resulted in more significant responses than others. The shoot and root regeneration through callus was observed in the MS media supplemented with 4.0 mg/L 6-BA and 0.5 mg/L NAA with an 84% response rate after two weeks of incubation and an average 5.6 number of shoots per callus. Only root and shoot regeneration was observed using half-strength MS media with 2.0 mg/L 6-BA and 1.0 mg/L NAA and 6.0 mg/L 6-BA and 0.5 mg/L NAA, respectively, after two weeks. The plantlets acclimatization had an average of 80% survival rate. These in-vitro regenerated plants by direct shoot organogenesis and through callus induction methods might aid in harvesting a bulk amount of secondary metabolites without destroying the native habitat. Thus, the methods would lead to environmental restoration sustainably.

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Effects of sterilization methods and plant growth regulators on in vitro regeneration and tuberization in Gloriosa superba (L.)

Cited by 5 publications

References 41 publications

Phytochemical analysis and enhanced production of alkaloids in non-dormant corm-derived callus of Gloriosa superba (L.) using plant growth regulators and abiotic elicitors

Phytochemical analysis and enhanced production of alkaloids in non-dormant corm-derived callus of Gloriosa superba (L.) using plant growth regulators and abiotic elicitors

Development of efficient, cost-effective in vitro micropropagation technique for threatened ethnomedicinal plant Clerodendrum indicum (L.) O. Kuntze

Development of efficient, cost-effective in vitro micropropagation technique for threatened ethnomedicinal plant Clerodendrum indicum (L.) O. Kuntze

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