Effects of sterilization and storage on respiration, nitrogen status and direct counts of soil bacteria using acridine orange

Ramsay, Angela J.; Bawden, A.D.

doi:10.1016/0038-0717(83)90069-x

Cited by 66 publications

(31 citation statements)

References 27 publications

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“…Stillfunctioning oxidative enzymes in these dead or damaged microbes may be responsible for the initial release of carbon dioxide after rewetting (Miller et al, 2005;Burns et al, 2013), even if the actual growth of viable cells is very low. Still-functioning enzymes can also explain consistent and significant levels of respiration during at least 3 months after all cells have been killed by irradiation (Ramsay and Bawden, 1983). A similar mechanism has furthermore been suggested for the initial decoupling between growth and respiration at higher than optimum growth temperatures (Pietik€ ainen et al, 2005).…”

Section: Decoupling Of Respiration and Growthmentioning

confidence: 92%

Prolonged drought changes the bacterial growth response to rewetting

Meisner

Rousk

Bååth

2015

Soil Biology and Biochemistry

124

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Section: Decoupling Of Respiration and Growthmentioning

confidence: 92%

Prolonged drought changes the bacterial growth response to rewetting

Meisner

Rousk

Bååth

2015

Soil Biology and Biochemistry

124

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“…After soil samples stored at 4 C were sterilized by gamma ray (60 kGy) irradiation, a 200 g portion sterilized soil was placed into a 500 mL bottle and allowed to stabilize for 8 weeks at room temperature (Ramsay and Bawden, 1983). Next, sterile water was added to maintain a constant moisture level (30% of field capacity), and the samples were pre-incubated at 20 C in the dark for 2 weeks before being tested for sterility.…”

Section: Microbial Community Swapping and Incubatingmentioning

confidence: 99%

Environmental conditions rather than microbial inoculum composition determine the bacterial composition, microbial biomass and enzymatic activity of reconstructed soil microbial communities

Xun

Huang

Zhao

et al. 2015

Soil Biology and Biochemistry

122

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“…Soil sterility was checked by plating serial dilutions on nutrient agar (BioMerieux, Lyon, France). As gamma irradiation produces double‐strand breaks in DNA but does not induce cell lysis, remaining dead Nitrobacter cells could interfere with our counts [17, 18]. Consequently, we checked that after a 8 week storage, no cell was detected by the immunofluorescence technique described in .6 in the non‐inoculated soil.…”

Section: Methodsmentioning

confidence: 99%

Activity, size and structure of a Nitrobacter community as affected by organic carbon and nitrite in sterile soil

Degrange¹,

Lensi²,

Bardin³

2006

FEMS Microbiology Ecology

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Nitrobacter is the most common nitrite‐oxidizing genus isolated from natural environments. In soil, several Nitrobacter strains can coexist. It is now well known that in pure cultures these strains are able to use organic carbon for their growth with highly variable efficiencies. Our purpose was to examine the role of nitrite or organic carbon as factors governing the evolution of the structure, the density and the activity of a Nitrobacter community in soil. This study has been performed on a recreated nitrite‐oxidizing community consisting of 3 Nitrobacter strains co‐inoculated into sterile soil and differing in their metabolic potentialities: a preferentially autotrophic strain (N. agilis strain AG), a preferentially mixotrophic strain (Nitrobacter genomic species 2 strain LL) and a preferentially heterotrophic strain (N. hamburgensis strain X14). Whatever the enrichments performed (0.271 or 1.353 mg NO2−‐N g−1 dry soil or 1 mg sodium acetate g−1 dry soil), we observed a first phase of multiplication of the total Nitrobacter cells until day 7 or 15, and then, a stabilization of the community until the end of the experiment (day 48). The growth and level of stabilization of this Nitrobacter community as well as the competition between strains in the first phase were predominantly controlled by soil nitrite concentration, resulting in a dominance of the more autotrophic strains. The stabilization of the community resulted from a drastic decrease in nitrite‐oxidation activity due not only to a direct nitrite limitation but also probably to a limiting O2 effect of the cells' ability to utilize nitrite. The competition was then predominantly monitored by strains' ability to utilize organic carbon for aerobic or anaerobic respiration. The autotrophy/heterotrophy shift strongly modified the result of the competition obtained at the end of the growth stage by inducing drastic declines of the more autotrophic strain and allowing continuous growth of the more heterotrophic strain. This suggests that heterotrophy being more tolerant than autotrophy to environmental changes, organic carbon could be a more permanent structuring factor of the Nitrobacter community than nitrite in soil.

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Effects of sterilization and storage on respiration, nitrogen status and direct counts of soil bacteria using acridine orange

Cited by 66 publications

References 27 publications

Prolonged drought changes the bacterial growth response to rewetting

Prolonged drought changes the bacterial growth response to rewetting

Environmental conditions rather than microbial inoculum composition determine the bacterial composition, microbial biomass and enzymatic activity of reconstructed soil microbial communities

Activity, size and structure of a Nitrobacter community as affected by organic carbon and nitrite in sterile soil

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