Effects of stem cell therapy on protein profile of parkinsonian rats using an¹⁸O‐labeling quantitative proteomic approach

Abstract

The application of neural stem cell (NSC) research to neurodegenerative diseases has led to promising clinical trials. Currently, NSC therapy is most promising for Parkinson's disease (PD). We conducted behavioral tests and immunoassays for the profiling of a PD model in rats to assess the therapeutic effects of NSC treatments. Further, using a multiple sample comparison workflow, combined with (18) O-labeled proteome mixtures, we compared the differentially expressed proteins from control, PD, and NSC-treated PD rats. The results were analyzed bioinformatically and verified by Western blot. Based on our initial findings, we believe that the proteomic approach is a valuable tool in evaluating the therapeutic effects of NSC transplantation on neurodegenerative disorders.

Quantitative Proteomics Using 18O Labeling on Target Peptides and Unlabeled Standards

“…18 O labeling could produce incomplete 18 O incorporation, bringing a mixture of peptides having either 16 O 2 , 18 O 1 , or 18 O 2 at C‐termini. That being said, this approach remains popular in global proteomics 26–28 and biomarker discovery studies 29,30 …”

Enzymatic Reactor with Trypsin Immobilized on Graphene Oxide Modified Polymer Microspheres To Achieve Automated Proteome Quantification

“…Furthermore, since protein digestion and peptide 18 O labeling could be achieved in H 2 18 O without adding extra labeling reagents, the secondary reactions inherent to other chemical labeling strategies, such as dimethyl labeling, 3 isobaric tags for relative and absolute quantitation (iTRAQ), 4 tandem mass tags (TMT), 5 and isotope-coded affinity tags (ICAT) 6 can be avoided. Therefore, in recent years, this strategy has been widely applied into relative proteome quantification, 7 de novo sequencing, 8 posttranslational modification analysis, 9 crosslinking chemistry, 10 and multiple reaction monitoring based absolute proteome quantification. 11 The main challenges of the direct 18 O labeling methods are the incomplete labeling and back exchange from 18 O to 16 O.…”