Effects of static magnetic field on human leukemic cell line HL-60

Sabó, Ján; Mirossay, Ladislav; Horovcak, L.; Šarišský, Marek; Miroššay, A.; Mojžiš, Ján

doi:10.1016/s1567-5394(02)00027-0

Cited by 70 publications

(52 citation statements)

References 7 publications

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“…Several experiments have been conducted to explore the interaction between SMF/PMF and antitumor drugs on biological systems (Gray et al 2000;Ruiz-Gomez et al 2002;Sabo et al 2002;Tofani et al 2003). In this study, we reported the biological effects of 8.8 mT SMF and paclitaxel on human myelogenous leukemia K562 cells in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…For example, Tofani and his colleagues (Tofani et al 2003) reported that the survival time of mice treated with the combination of cisplatin and 3 mT SMF is significantly longer than that of mice treated with cisplatin or SMF alone after mice are cultivated with murine Lewis lung carcinomas (LLCs). In the in vitro cell growth assay, the application of 1 T SMF, which is combined with a mixture of antineoplastic drugs (5-fluorouracil, cisplatin, doxorubicin and vincristine) for 72 h on HL-60 cells, enhances the cytotoxic effect of antineoplastic drugs (Sabo et al 2002). These findings indicate that the cytotoxic effects of SMF/PMF plus antitumor drugs are stronger than those of SMF/PMF or chemotherapeutic drugs alone.…”

Section: Introductionmentioning

confidence: 91%

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Biologic effects of SMF and paclitaxel on K562 human leukemia cells

Sun

Chen²,

Qi³

et al. 2012

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Abstract. In this study, we evaluated the ability of 8.8 mT static magnetic fields (SMF) to enhance the in vitro action of a chemotherapeutic agent, paclitaxel, against K562 human leukemia cells. We analyzed the cell proliferation, cell cycle distribution, DNA damage and alteration of cell surface and cell organelle ultrastructure after K562 cells were exposed to paclitaxel in the presence or absence of 8.8 mT SMF. The results showed that in the presence of SMF, the efficient concentration of paclitaxel on K562 cells was decreased from 50 to 10 ng/ml. Cell cycle analysis indicated that K562 cells treated with SMF plus paclitaxel were arrested at the G2 phase, which was mainly induced by paclitaxel. Through comet assay, we found that the cell cycle arrest effect of paclitaxel with or without SMF on K562 cells was correlated with DNA damage. The results of atomic force microscopy and transmission electron microscopy observation showed that the cell ultrastructure was altered in the group treated with the combination of SMF and paclitaxel, holes and protuberances were observed, and vacuoles in cytoplasm were augmented. Our data indicated that the potency of the combination of SMF and paclitaxel was greater than that of SMF or paclitaxel alone on K562 cells, and these effects were correlated with DNA damage induced by SMF and paclitaxel. Therefore, the alteration of cell membrane permeability may be one important mechanism underlying the effects of SMF and paclitaxel on K562 cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Biologic effects of SMF and paclitaxel on K562 human leukemia cells

Sun

Chen²,

Qi³

et al. 2012

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“…11 Nevertheless, other reports present more promising results in vitro and in vivo. [12][13][14] Interestingly, Gray et al, reported enhancement of adriamycin chemotherapy in combination with SMF exposure in mice bearing 16/C murine mammary adenocarcinoma. 15 However, the mechanism of this effect remained unclear.…”

Section: Research Papermentioning

confidence: 99%

Static magnetic fields induce blood flow decrease and platelet adherence in tumor microvessels

Strieth

Strelczyk²,

Eichhorn³

et al. 2008

Cancer Biology & Therapy

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Red blood cell flow in striated muscle capillaries is reduced during exposure to strong static magnetic fields (SMFs). Intratumoral microcirculation is characterized by tortuous microvessels with chaotic architecture and by irregular, sluggish blood flow with unstable rheology.It was the aim of this study to analyze SMF exposure effects on tumor microcirculation with regard to interactions of corpuscular blood components with tumor microvessel walls. In vivo fluorescence microscopy was performed in A-Mel-3 tumors growing in dorsal skinfold chamber preparations of Syrian Golden hamsters. SMFs with varying field strength (<600 mT) were generated by changing the distance between a strong NdFeB rod magnet and the tissue region of interest.Short-time exposure above a magnetic flux density of about 150 mT resulted in a significant reduction of red blood cell velocity (v RBC ) and segmental blood flow in tumor microvessels. At the maximum strength of 587 mT, a reversible reduction of v RBC (~40%) and of functional vessel densitiy (~15%) was observed.Prolongation of the exposure time from one minute to up to 3 h resulted in comparable reductions. Microvessel diameters and leukocyte-endothelial cell interactions remained unaffected by SMF exposure. However, in contrast to tumor-free striated muscle controls, exposure at the maximum flux density induced a significant increase in platelet-endothelial cell adherence in a time-dependent manner that was reversible after reducing SMF strength.These reversible changes may have implications for functional measurements of tumor microcirculation by MRI and new therapeutic strategies using strong SMFs.

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“…Alternating MF mostly cause an increase in Ca 2+ in many cell types [Carson et al, 1990;Lyle et al, 1991;Lindstrom et al, 1993;Fitzsimmons et al, 1994;Lindstrom et al, 1995;Barbier et al, 1996;Pessina et al, 2001]. However, a decrease in Ca 2+ for signaling was also reported with retardation of metabolic activity and block of action potentials [Cavopol, 1995;Rosen, 1996;Sabo et al, 2002].…”

Section: Coupling Of Emf To Molecular Reactionsmentioning

confidence: 99%

Effects of Electromagnetic Fields on Cells: Physiological and Therapeutical Approaches and Molecular Mechanisms of Interaction

Funk

Monsees²

2006

Cells Tissues Organs

143

104

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This review concentrates on findings described in the recent literature on the response of cells and tissues to electromagnetic fields (EMF). Models of the causal interaction between different forms of EMF and ions or biomolecules of the cell will be presented together with our own results in cell surface recognition. Naturally occurring electric fields are not only important for cell-surface interactions but are also pivotal for the normal development of the organism and its physiological functions. A further goal of this review is to bridge the gap between recent cell biological studies (which, indeed, show new data of EMF actions) and aspects of EMF-based therapy, e.g., in wounds and bone fractures.

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Effects of static magnetic field on human leukemic cell line HL-60

Cited by 70 publications

References 7 publications

Biologic effects of SMF and paclitaxel on K562 human leukemia cells

Biologic effects of SMF and paclitaxel on K562 human leukemia cells

Static magnetic fields induce blood flow decrease and platelet adherence in tumor microvessels

Effects of Electromagnetic Fields on Cells: Physiological and Therapeutical Approaches and Molecular Mechanisms of Interaction

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