Effects of starvation on the carbohydrate and lipid metabolism in crabs previously maintained on a high protein or carbohydrate-rich diet

Vinagre, Anapaula Sommer; Silva, Roselis S.M. da

doi:10.1016/0300-9629(92)90213-a

Cited by 60 publications

(18 citation statements)

References 15 publications

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“…Other studies have confirmed that glycogen stores decrease with increasing lactate concentration in the hemolymph (Hill et al, 1991;Sneddon et al, 1999;Taylor & Spicer, 1987), indicating glycogenolysis, rather than gluconeogenesis, as the main source of glucose for glycolysis. Moreover, diet was found to directly affect crab hemolymph glucose levels, as well as hepatopancreas and muscle glycogen concentrations (Marqueze et al, 2011;Vinagre & Da Silva, 1992). Therefore, some individual P. samuelis crabs may have exhibited ineffective anaerobic metabolism due to insufficient glycogen stores, and subsequently died quickly with relatively moderate hemolymph lactate levels.…”

Section: Discussionmentioning

confidence: 99%

Lactate accumulation in the intertidal hermit crab, <i>Pagurus samuelis</i>, in response to burial-induced hypoxia

2017

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Abstract. We subjected the intertidal hermit crab, Pagurus samuelis (Stimpson, 1857), to various treatments to determine physiological responses of this species to the environmental stress of burial. Hermit crabs were buried with 6 cm of sediment and excavated at 2 h intervals up to a maximum of 12 h. Duration of burial and state (alive or dead) of the crab were analyzed for effects on lactate accumulation in hemolymph. Hermit crab weight, shell weight, weight ratio, lactate, and burial duration were analyzed for their influence on survival. As expected, lactate levels, as well as incidence of death, rose with duration of burial. Significant interaction, however, was also found between burial duration, crab state, and lactate concentration. There was a trend for lactate concentration to be low for surviving crabs, yet higher for dead crabs during shorter burial durations. Conversely and surprisingly, lactate concentrations were very high in surviving crabs, yet lower in dead crabs during long burial durations. Since some surviving crabs were able to develop very high lactate levels, we suggest that lactate buildup itself is unlikely to be the sole cause of death. Further studies are needed to identify factors affecting crab resilience during the stress of burial.

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Section: Discussionmentioning

confidence: 99%

Lactate accumulation in the intertidal hermit crab, <i>Pagurus samuelis</i>, in response to burial-induced hypoxia

2017

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“…Furthermore, the extent to which each compound class contributes to meet the energy demand during such a period can be estimated. In crustacean muscles, glycogen and lipids are generally mobilised first, when energy is required, but proteins can also be catabolised (Vinagre and da Silva 1992;Ceccaldi 2006).…”

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confidence: 99%

Physiological response of adult Antarctic krill, Euphausia superba, to long-term starvation

et al. 2015

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Adult Euphausia superba survive winter without or with little feeding. It is not exactly known whether the scarcity of food or an internal clock, set by the natural Antarctic light regime, are responsible for non-feeding. Our research questions were therefore the following: (1) How will physiological and biochemical conditions of krill change during long-term starvation at constant light regime? (2) If and how do enzyme activities change during such starvation? (3) What is the influence of food availability versus that of light regime? To answer these questions, adult krill were starved under laboratory conditions for 12 weeks with constant light regime (12:12; dark/light) and the impact on physiological functions was studied. Initial experimental condition of krill resembled the condition of late spring krill in the field with fully active metabolism and low lipid reserves. Metabolic activity and activities of enzymes catabolising lipids decreased after the onset of starvation and remained low throughout, whereas lipid reserves declined and lipid composition changed. Mass and size of krill decreased while the inter-moult period increased. Depletion of storage-and structural metabolites occurred in the order of depot lipids and glycogen reserves after onset of starvation until proteins were almost exclusively used after 6-7 weeks of starvation. Results confirmed various proposed overwintering mechanisms such as metabolic slowdown, slow growth or shrinkage and use of lipid reserves. However, these changes were set in motion by food shortage only, i.e. without the trigger of a changing light regime.

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“…Several studies have verified that different diets (HC and HP) modify significantly the concentrations of free glucose and glycogen in tissues of C. granulatus (KUCHARSKI & DA SILVA, 1991a;VINAGRE & DA SILVA, 1992;OLIVEIRA et al, 2004a, b).…”

Section: Discussionmentioning

confidence: 92%

“…Stable glucose haemolymph levels are essential for the regular functioning of the nervous, muscle, and reproductive systems (VERRI et al, 2001;RADFORD et al, 2005). Glucose can be accumulated in the form of free glucose and glycogen in the hepatopancreas and in other tissues, such as the muscles and the gills (VINAGRE & DA SILVA, 1992;OLIVEIRA et al, 2003).…”

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confidence: 99%

Seasonal variation of the effect of high-carbohydrate and high-protein diets on the intermediate metabolism of Parastacus brasiliensis (Crustacea, Decapoda, Parastacidae) maintained in the laboratory

Dutra

Silva

Zank

et al. 2008

Iheringia, Sér. Zool.

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ABSTRACT. The goal of this study was to evaluate the effect of a high-carbohydrate diet (HC) and a high-protein diet (HP) on the metabolism of the crayfish Parastacus brasiliensis (Von Martens, 1869), collected in different seasons and maintained in the laboratory for 15 days. Crayfish were collected monthly from January 2002 to January 2004 at São Francisco de Paula, Southern Brazil, in Guarapirá stream. In the laboratory, the animals were kept submerged in aquariums under controlled conditions. They were fed ad libitum, for 15 days with either a HC or HP diet. At the end of this period, haemolymph samples were collected, as were hepatopancreas, gills, and abdominal muscle that were removed for determination of glycogen, free glucose, lipids, and triglycerides. The haemolymph samples were used for determination of glucose, proteins, lipids, and triglycerides. Statistical analysis (ANOVA) revealed significant seasonal differences in biochemical composition in crayfish maintained on HC or HP diets. Independent of the diets offered to the animals and the controlled conditions for 15 days, the indications of seasonality were unchanged. The observed changes seemed to be related to the reproductive period. Moreover, the HC diet increased all energy reserves in adult parastacids, which may aid in reproduction. KEYWORDS.Crayfish, experimental culture, diet, metabolism, seasonality. Após o final do período, as amostras de hemolinfa foram coletadas, assim como o hepatopâncreas, as brânquias e o músculo abdominal foram removidos para a determinação do glicogênio, glicose livre, lipídios e triglicerídeos. As amostras de hemolinfa foram usadas para a determinação da glicose, proteínas, lipídios e triglicerídeos. As análises estatísticas (ANOVA) revelaram uma diferença sazonal significativa na composição bioquímica nos lagostins mantidos com dieta HC ou HP. Independente da dieta oferecida para os animais e das condições controladas por 15 dias, não foi possível alterar os perfis metabólicos encontrados sazonalmente nos animais em campo. As mudanças observadas parecem estar relacionadas ao período reprodutivo. Além disso, a dieta HC aumentou todas as reservas energéticas nos parastacídeos adultos, o que pode auxiliar na reprodução. RESUMO. Variações sazonais do efeito de uma dieta rica em carboidrato e rica em proteínas no metabolismo intermediário de PALAVRAS-CHAVE.Lagostim, cultivo experimental, dieta, metabolismo e sazonalidade.

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Effects of starvation on the carbohydrate and lipid metabolism in crabs previously maintained on a high protein or carbohydrate-rich diet

Cited by 60 publications

References 15 publications

Lactate accumulation in the intertidal hermit crab, <i>Pagurus samuelis</i>, in response to burial-induced hypoxia

Lactate accumulation in the intertidal hermit crab, <i>Pagurus samuelis</i>, in response to burial-induced hypoxia

Physiological response of adult Antarctic krill, Euphausia superba, to long-term starvation

Seasonal variation of the effect of high-carbohydrate and high-protein diets on the intermediate metabolism of Parastacus brasiliensis (Crustacea, Decapoda, Parastacidae) maintained in the laboratory

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