Effects of standardized Cannabis sativa extract and ionizing radiation in melanoma cells in vitro

Naderi, Jamal; Dana, Nasim; Javanmard, Shaghayegh Haghjooy; Amooheidari, Alireza; Yahay, Maryam; Vaseghi, Golnaz

doi:10.4103/jcrt.jcrt_1394_16

Cited by 9 publications

(1 citation statement)

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“…CBD had comparable inhibitions on the growth of B16F10 cells at the time points of 24, 48, and 72 h (Figure 3A), whilst its antiproliferative effects were more significant for the longer treatment times (24 and 72 h) in A375 cells (Figure 3B). Although several cannabis extracts have been reported to exert inhibitory effects on the growth of melanoma cells [15,16], only limited studies explored the antiproliferative activity of the individual cannabinoids. A study reported that CBD and CBG showed the most promising antiproliferative effect (IC 50 = 12.0 and 12.1 µM, respectively) compared to other minor cannabinoids, such as CBN and CBC, in human melanoma A375 cells [17].…”

Section: Phytocannabinoids Inhibit the Proliferation Of Murine And Hu...mentioning

confidence: 99%

Bipiperidinyl Derivatives of Cannabidiol Enhance Its Antiproliferative Effects in Melanoma Cells

Lyu,

Li,

Wan

et al. 2024

Antioxidants

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Cannabis and its major cannabinoid cannabidiol (CBD) are reported to exhibit anticancer activity against skin tumors. However, the cytotoxic effects of other minor cannabinoids and synthetic CBD derivatives in melanoma are not fully elucidated. Herein, the antiproliferative activity of a panel of phytocannabinoids was screened against murine (B16F10) and human (A375) melanoma cells. CBD was the most cytotoxic natural cannabinoid with respective IC50 of 28.6 and 51.6 μM. Further assessment of the cytotoxicity of synthetic CBD derivatives in B16F10 cells identified two bipiperidinyl group-bearing derivatives (22 and 34) with enhanced cytotoxicity (IC50 = 3.1 and 8.5 μM, respectively). Furthermore, several cell death assays including flow cytometric (for apoptosis and ferroptosis) and lactate dehydrogenase (for pyroptosis) assays were used to characterize the antiproliferative activity of CBD and its bipiperidinyl derivatives. The augmented cytotoxicity of 22 and 34 in B16F10 cells was attributed to their capacity to promote apoptosis (as evidenced by increased apoptotic population). Taken together, this study supports the notion that CBD and its derivatives are promising lead compounds for cannabinoid-based interventions for melanoma management.

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Section: Phytocannabinoids Inhibit the Proliferation Of Murine And Hu...mentioning

confidence: 99%