Effects of spirulina supplementation on lipid metabolism disorder, oxidative stress caused by high-energy dietary in Hu sheep

Liang, Yaxu; Bao, Yongjin; Gao, Xiaoxiao; Deng, Kaiping; An, Shi; Wang, Zhibo; Huang, Xinai; Liu, Dong; Liu, Zhinan; Wang, Feng; Fan, Yixuan

doi:10.1016/j.meatsci.2020.108094

Cited by 24 publications

(16 citation statements)

References 47 publications

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“…Total RNA was extracted from AML-12 cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to our previous description. 15 RNA was reverse transcribed into cDNA using a kit (Cat NO. RR047A, TaKaRa Biotechnology Co., Ltd., Dalian, China).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

γ-Linolenic Acid Prevents Lipid Metabolism Disorder in Palmitic Acid-Treated Alpha Mouse Liver-12 Cells by Balancing Autophagy and Apoptosis via the LKB1-AMPK-mTOR Pathway

Liang

Zhang

et al. 2021

J. Agric. Food Chem.

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Excessive fat deposition is the main character in nonalcoholic fatty liver disease (NAFLD), while γ-linolenic acid (GLA) is a polyunsaturated fatty acid that can reduce lipid deposition. This study investigated the effect and regulatory mechanism of GLA (100 μM) on lipid metabolism in alpha mouse liver 12 (AML-12) cells treated by 400 μM palmitic acid (PA). GLA reduced lipid content and increased fatty acid β oxidation, as indicated by decreasing triglyceride and cholesterol contents and increasing mRNA and protein expressions of CPT1α and PPARα. GLA relieved oxidative stress caused by PA, upregulated mRNA levels of superoxide dismutase and glutathione peroxidase, and decreased reactive oxygen species content. GLA reduced apoptosis, as indicated by decreases in the BAX/BCL2 expression level and apoptosis percentage. GLA activated autophagy, autophagosome-lysosome fusion, and LKB1-AMPK-mTOR signaling and upregulated mRNA and protein expressions of Beclin-1, autophagy-related 5, and liver kinase B1 (LKB1). These effects of GLA on lipid metabolism disorders of PA-treated hepatocytes were reversed by autophagy inhibitor 3MA and AMPK inhibitor compound C, confirming our conclusions. Overall, GLA can protect AML-12 cells from lipid metabolism disorder caused by PA via balancing autophagy and apoptosis mediated by the LKB1-AMPK-mTOR pathway. Consequently, GLA, as a dietary supplement, can help to prevent and treat NAFLD by regulating lipid metabolism and autophagy.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

γ-Linolenic Acid Prevents Lipid Metabolism Disorder in Palmitic Acid-Treated Alpha Mouse Liver-12 Cells by Balancing Autophagy and Apoptosis via the LKB1-AMPK-mTOR Pathway

Liang

Zhang

et al. 2021

J. Agric. Food Chem.

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“…Intake of HE density diet has been recognized as one of the important causes of oxidative stress in animals such as pigeons (Hosseinian and Hasanzadeh, 2021), piglets (Adebowale et al, 2015), rats (Jiang and Jiang, 2018), cows (Wang et al, 2010), and sheep (Liang et al, 2020). This current study revealed that energy level and FA have no effect on plasma and breast muscle MDA and SOD levels in broiler chickens.…”

Section: Discussionmentioning

confidence: 44%

“…Large production of ROS results in lipid peroxidation, for which malondialdehyde (MDA) is an important indicator. Many literature have discussed extensively the oxidative response of animals when exposed to a high energy density diet Liang et al (2020). reported that serum GPX activity and total antioxidant capacity (T-AOC) and liver SOD and CAT significantly decreased in lambs fed a high energy density diet compared to those fed a normal diet.…”

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confidence: 99%

Effect of dietary folic acid and energy density on immune response, gut morphology, and oxidative status in blood and breast muscle of broiler chickens

Akinyemi

Adewole

2022

Can. J. Anim. Sci.

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Folic acid (FA) plays an essential role in the functioning of the gut and serves as an antioxidant. Therefore, dietary supplementation with high levels of FA may improve gut health and prevent oxidative stress caused by feeding a high energy density diet to broiler chickens. Broiler chickens were assigned into eight treatments, consisting of either a normal energy (NE) or high energy (HE) density diet, and four FA levels (2.2, 5, 10, and 15 ppm). Data were analyzed by SAS 16 GLM procedure. Birds fed HE diets had increased (P<0.05) plasma concentrations of calcium and albumin but reduced (P<0.005) weights of ceca and bursa compared to those fed NE diets. Dietary supplementation with 10ppm FA significantly increased (P<0.05) birds’ heart weight and bile acid concentration. FA and energy density interactions were significant for jejunal villus height (VH; P=0.0226), villus width (VW; P<0.0001), and crypt depth (CD; P=0.0332). Among the NE group, birds fed 5-15ppm FA had reduced (P<.0001) VW, while in the HE groups, 15ppm FA supplementation resulted in an increased jejunal VH (P=0.0317) compared to other treatments. In conclusion, dietary supplementation with increased levels of FA in HE diets could be beneficial for the intestinal health of broiler chickens.

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“…The detail information of the meat quality detection was according to a previous study of Liang et al [ 54 ]. In brief, body fat (assessed as GR) value was assessed by measuring the thickness at the 12th/13th rib intersection 110 mm away from the midline using a vernier caliper.…”

Section: Methodsmentioning

confidence: 99%

Effects of mulberry leaf silage on antioxidant and immunomodulatory activity and rumen bacterial community of lambs

Wang

Luo

2021

BMC Microbiol

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Background Rumen is a natural fermentation system and the microorganisms inside can effectively utilize plant bioresource and interact with host metabolism. Here, analysis of rumen microbiome, together with animal performance and serum metabolism in a lamb model were performed to identify the potential use of mulberry leaf silage (MS) to replace alfalfa silage (AS) as a new functional feed resource and to mining the novel specific mulberry leaf associated rumen bacteria interact with host metabolism. Results The lambs fed with MS diet showed improved antioxidant capacity and immune function compared to those fed AS diet. The MS diet significantly altered rumen microbiota α- and β-diversity and taxonomic composition. Microbial analysis revealed that Bifidobacterium, Lactobacillus and Schwartzia were enhanced, and Ruminococcaceae UCG-010 and Lachnospiraceae_XPB1014_group were down-regulated in the rumen of MS group. A strong association was also found between these rumen microbial taxa and host antioxidant and immunomodulatory capacity. Conclusion These findings indicated that mulberry leaf silage can be a high-quality feed source or bioactive pharmaceutical that is responsible for ruminant’s health benefits. The modified rumen microbial community by mulberry leaf silage were associated with the enhanced antioxidant capacity and immunomodulatory of lambs.

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Effects of spirulina supplementation on lipid metabolism disorder, oxidative stress caused by high-energy dietary in Hu sheep

Cited by 24 publications

References 47 publications

γ-Linolenic Acid Prevents Lipid Metabolism Disorder in Palmitic Acid-Treated Alpha Mouse Liver-12 Cells by Balancing Autophagy and Apoptosis via the LKB1-AMPK-mTOR Pathway

γ-Linolenic Acid Prevents Lipid Metabolism Disorder in Palmitic Acid-Treated Alpha Mouse Liver-12 Cells by Balancing Autophagy and Apoptosis via the LKB1-AMPK-mTOR Pathway

Effect of dietary folic acid and energy density on immune response, gut morphology, and oxidative status in blood and breast muscle of broiler chickens

Effects of mulberry leaf silage on antioxidant and immunomodulatory activity and rumen bacterial community of lambs

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