Effects of specific organs on seed oil accumulation in Brassica napus L.

Liu, Jing; Hua, Wei; Yang, Hongli; Guo, Tingting; Sun, Xingchao; Wang, Xinfa; Liu, Guihua; Wang, Hanzhong

doi:10.1016/j.plantsci.2014.06.017

Cited by 22 publications

(12 citation statements)

References 44 publications

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“…The seed coat is entirely maternal in origin and has been implicated in the control of seed oil synthesis (Radchuk and Borisjuk, 2014). For instance, there is evidence to suggest that the sugar concentration in the seed coat might regulate the efficiency of oil accumulation in the embryo (Liu et al, 2014). We noted that the seed coat/ aleurone layer contained a high proportion of 18:1 n7 (vaccenic acid) compared with 18:1 n9 (oleic acid).…”

Section: The Seed Coat/aleurone Layer Has a Distinct Lipid Compositionmentioning

confidence: 79%

Spatial and Temporal Mapping of Key Lipid Species in Brassica napus Seeds

et al. 2017

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The regulation of lipid synthesis in oil seeds is still not fully understood. Oilseed rape (Brassica napus) is the third most productive vegetable oil crop on the global market; therefore, increasing our understanding of lipid accumulation in oilseed rape seeds is of great economic, as well as intellectual, importance. Matrix-assisted laser/desorption ionization-mass spectrometry imaging (MALDI-MSI) is a technique that allows the mapping of metabolites directly onto intact biological tissues, giving a spatial context to metabolism. We have used MALDI-MSI to study the spatial distribution of two major lipid species, triacylglycerols and phosphatidylcholines. A dramatic, heterogenous landscape of molecular species was revealed, demonstrating significantly different lipid compositions between the various tissue types within the seed. The embryonic axis was found to be particularly enriched in palmitic acid, while the seed coat/aleurone layer accumulated vaccenic, linoleic, and a-linoleic acids. Furthermore, the lipid composition of the inner and outer cotyledons differed from each other, a remarkable discovery given the supposed identical functionality of these two tissues. Triacylglycerol and phosphatidylcholine molecular species distribution was analyzed through a developmental time series covering early seed lipid accumulation to seed maturity. The spatial patterning of lipid molecular species did not vary significantly during seed development. Data gathered using MALDI-MSI was verified through gas chromatography analysis of dissected seeds. The distinct lipid distribution profiles observed imply differential regulation of lipid metabolism between the different tissue types of the seed. Further understanding of this differential regulation will enhance efforts to improve oilseed rape productivity and quality.

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Section: The Seed Coat/aleurone Layer Has a Distinct Lipid Compositionmentioning

confidence: 79%

Spatial and Temporal Mapping of Key Lipid Species in Brassica napus Seeds

et al. 2017

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“…Previous studies have shown that the photosynthesis in silique wall makes a crucial contribution to OC [6, 12, 16, 17], providing an explanation for the influence of maternal parent on OC. Therefore, the selection for high-oil rapeseed would be more effective based on the photosynthetic activity of silique wall than based on the performance of maternal parent.…”

Section: Discussionmentioning

confidence: 99%

“…Seed lipid synthesis is independent of the leaf photosynthesis and the phloem transport of photosynthate [16], but mainly requires the supply of photosynthate from the silique wall [6, 12]. Photosynthesis of the silique wall, sugar transport in the seed coat, and the expression of fatty acid synthesis-related genes in the embryo can significantly influence the OC [17, 18]. In addition, the storage substance in seed is determined not only by the availability of assimilates (source strength), but also by the intrinsic traits of the seed (sink strength), which are controlled by the embryo genotype [19].…”

Section: Introductionmentioning

confidence: 99%

Genetic effects and genotype × environment interactions govern seed oil content in Brassica napus L.

Guo

Wang

et al. 2017

BMC Genet

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BackgroundAs seed oil content (OC) is a key measure of rapeseed quality, better understanding the genetic basis of OC would greatly facilitate the breeding of high-oil cultivars. Here, we investigated the components of genetic effects and genotype × environment interactions (GE) that govern OC using a full diallel set of nine parents, which represented a wide range of the Chinese rapeseed cultivars and pure lines with various OCs.ResultsOur results from an embryo-cytoplasm-maternal (GoCGm) model for diploid seeds showed that OC was primarily determined by genetic effects (VG) and GE (VGE), which together accounted for 86.19% of the phenotypic variance (VP). GE (VGE) alone accounted for 51.68% of the total genetic variance, indicating the importance of GE interaction for OC. Furthermore, maternal variance explained 75.03% of the total genetic variance, embryo and cytoplasmic effects accounted for 21.02% and 3.95%, respectively. We also found that the OC of F1 seeds was mainly determined by maternal effect and slightly affected by xenia. Thus, the OC of rapeseed was simultaneously affected by various genetic components, including maternal, embryo, cytoplasm, xenia and GE effects. In addition, general combining ability (GCA), specific combining ability (SCA), and maternal variance had significant influence on OC. The lines H2 and H1 were good general combiners, suggesting that they would be the best parental candidates for OC improvement. Crosses H3 × M2 and H1 × M3 exhibited significant SCA, suggesting their potentials in hybrid development.ConclusionsOur study thoroughly investigated and reliably quantified various genetic factors associated with OC of rapeseed by using a full diallel and backcross and reciprocal backcross. This findings lay a foundation for future genetic studies of OC and provide guidance for breeding of high-oil rapeseed cultivars.

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“…For example, AP2 and KLU regulate glucose metabolism, and affect the seed oil content in the Arabidopsis seed coat [41,42,53] . Tissue culture studies using rapeseed siliques, ovules and embryos from rapeseed lines with different oil content have confirmed that seed coat affects oil accumulation by regulating sucrose concentration [44,54] . TRANSPARENT TESTA 8 (TT8) in the seed coat was also reported to act maternally to affect seed FA biosynthesis and inhibited seed FA accumulation by downregulating a group of genes including LEC1, LEC2, FUS3, and CDS2, which are transcriptional factors important for seed development or FA biosynthesis [43] .…”

Section: Maternal Effects On Seed Oil Accumulationmentioning

confidence: 93%

Molecular regulation and genetic improvement of seed oil content in Brassica napus L.

Hua¹,

Liu²,

Wang³

2016

Front. Agr. Sci. Eng.

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As an important oil crop and a potential bioenergy crop, Brassica napus L. is becoming a model plant for basic research on seed lipid biosynthesis as well as seed oil content, which has always been the key breeding objective. In this review, we present current progress in understanding of the regulation of oil content in B. napus, including genetics, biosynthesis pathway, transcriptional regulation, maternal effects and QTL analysis. Furthermore, the history of breeding for high oil content in B. napus is summarized and the progress in breeding ultra-high oil content lines is described. Finally, prospects for breeding high oil content B. napus cultivars are outlined.

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Effects of specific organs on seed oil accumulation in Brassica napus L.

Cited by 22 publications

References 44 publications

Spatial and Temporal Mapping of Key Lipid Species in Brassica napus Seeds

Spatial and Temporal Mapping of Key Lipid Species in Brassica napus Seeds

Genetic effects and genotype × environment interactions govern seed oil content in Brassica napus L.

Molecular regulation and genetic improvement of seed oil content in Brassica napus L.

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