Effects of Space Mission Factors on the Morphology and Function of Endothelial Cells

Kapitonova, Marina; Кузнецов, С. Л.; Froemming, Gabriele Ruth Anisah; Muid, Suhaila Abd; Нор-Ашикин, М. Н. К.; Otman, S.; Shahir, A. R. M.; Nawawi, H.

doi:10.1007/s10517-013-2059-7

Cited by 16 publications

(15 citation statements)

References 10 publications

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“…Briefly, in cultured HUVEC, real microgravity affects the same molecular machinery which senses alterations of flow and generates a prooxidative environment that alters endothelial function and promotes senescence [ 21 ]. Similar conclusions were reached by Kapitonova et al [ 30 , 58 ], who described premature senescence in space-flown HUVEC. By accelerating some aspects of senescence, microgravity offers a big challenge to study the mechanisms implicated in the onset of aging.…”

Section: The Effects Of Microgravity On Ecssupporting

confidence: 90%

“…The controversial results reported about the response of ECs to microgravity could be due also to the diverse experimental approaches utilized, such as the device simulating microgravity, the duration of exposure to simulated microgravity, and the degree of reduction of the gravity that can be reached operating these devices differently (see above). Nevertheless, altered EC morphology, cell membrane permeability and senescence are documented by spaceflight experiments on cultured endothelium [ 21 , 30 , 58 ].…”

Section: The Effects Of Microgravity On Ecsmentioning

confidence: 99%

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The Impact of Microgravity and Hypergravity on Endothelial Cells

Maier

Cialdai

Monici

et al. 2015

BioMed Research International

115

118

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The endothelial cells (ECs), which line the inner surface of vessels, play a fundamental role in maintaining vascular integrity and tissue homeostasis, since they regulate local blood flow and other physiological processes. ECs are highly sensitive to mechanical stress, including hypergravity and microgravity. Indeed, they undergo morphological and functional changes in response to alterations of gravity. In particular microgravity leads to changes in the production and expression of vasoactive and inflammatory mediators and adhesion molecules, which mainly result from changes in the remodelling of the cytoskeleton and the distribution of caveolae. These molecular modifications finely control cell survival, proliferation, apoptosis, migration, and angiogenesis. This review summarizes the state of the art on how microgravity and hypergravity affect cultured ECs functions and discusses some controversial issues reported in the literature.

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Section: The Effects Of Microgravity On Ecssupporting

confidence: 90%

Section: The Effects Of Microgravity On Ecsmentioning

confidence: 99%

The Impact of Microgravity and Hypergravity on Endothelial Cells

Maier

Cialdai

Monici

et al. 2015

BioMed Research International

115

118

View full text Add to dashboard Cite

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“…In the case of whole-cell analysis, this differentiation disappears. It is a known fact that studied cytoskeletal structures, as well as the cells themselves, are dynamic objects and their behavior and morphology depend on several different factors that are not included in the study [ 57 , 58 , 59 , 60 ]. However, during the experiments, efforts were made to minimize the influence of external factors through identical culturing conditions, passage and staining protocols, or microscopic observation parameters itself.…”

Section: Resultsmentioning

confidence: 99%

Insights into In Vitro Wound Closure on Two Biopolyesters—Polylactide and Polyhydroxyoctanoate

et al. 2020

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Two bio-based polymers have been compared in this study, namely: polylactide (PLA) and polyhydroxyoctanoate (PHO). Due to their properties such as biocompatibility, and biointegrity they are considered to be valuable materials for medical purposes, i.e., creating scaffolds or wound dressings. Presented biopolymers were investigated for their impact on cellular migration strategies of mouse embryonic fibroblasts (MEF) 3T3 cell line. Advanced microscopic techniques, including confocal microscopy and immunofluorescent protocols, enabled the thorough analysis of the cell shape and migration. Application of wound healing assay combined with dedicated software allowed us to perform quantitative analysis of wound closure dynamics. The outcome of the experiments demonstrated that the wound closure dynamics for PLA differs from PHO. Single fibroblasts grown on PLA moved 1.5-fold faster, than those migrating on the PHO surface. However, when a layer of cells was considered, the wound closure was by 4.1 h faster for PHO material. The accomplished work confirms the potential of PLA and PHO as excellent candidates for medical applications, due to their properties that propagate cell migration, vitality, and proliferation—essential cell processes in the healing of damaged tissues.

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“…While the altered morphology is documented by previous spaceflight experiments on the cultured endothelium, it is noted that those data are collected from human umbilical vein endothelial cells (HUVEC) cultured on microcarrier beads in a postflight observation. 15,16 Regardless of different types of ECs, other factors may also be involved in the differences between ours and those in the literature, including the material of the culture chamber, the curvature of the microcarrier, and the mission of timeliness. Meanwhile, rMSCs in space or on the ground are all grown up with time from an incomplete to a compact monolayer.…”

Section: B Scientific Data Acquisitionmentioning

confidence: 72%

An integration design of gas exchange, bubble separation, and flow control in a space cell culture system on board the SJ-10 satellite

Sun

Wang

Yang

et al. 2019

Review of Scientific Instruments

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Pathophysiological changes of astronauts under space microgravity involve complex factors and require an integrative perspective to fully understand the mechanisms. The readouts from space cell biology experiments strongly depend on the hardware and especially the cell bioreactor that is used in distinct spacecraft. Herein, a specialized cell culture bioreactor is designed for culturing mammalian cells on board the SJ-10 satellite. This hardware focuses mainly on satisfying the requirements of gas exchange, bubble separation, and flow control, as well as their functional and structural integration on cell culture within the technical and environmental constraints of the spacecraft platform under microgravity. A passive bubble separator is constructed and is connected in series to an individual cell culture chamber to remove the bubbles that were produced in orbit during cell growth. A moderate flow rate is preset to provide sufficient mass transfer and low shear stress in a well-designed flow circuit. Together with other modules of temperature control, in situ microscopic imaging, and online imaging acquisition, this novel space cell culture system is successfully used to culture human endothelial cells and rat bone marrow-derived mesenchymal stem cells in the SJ-10 mission. The advantages and shortcomings of the integration design are discussed for this type of the hardware.

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Effects of Space Mission Factors on the Morphology and Function of Endothelial Cells

Cited by 16 publications

References 10 publications

The Impact of Microgravity and Hypergravity on Endothelial Cells

The Impact of Microgravity and Hypergravity on Endothelial Cells

Insights into In Vitro Wound Closure on Two Biopolyesters—Polylactide and Polyhydroxyoctanoate

An integration design of gas exchange, bubble separation, and flow control in a space cell culture system on board the SJ-10 satellite

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