“…On the other hand, in secondary transformation agro-industries, the products, by-products and residuals of the first transformation are usually used as starting Uses of the Response Surface Methodology for the Optimization of Agro-Industrial Processes DOI: http://dx.doi.org/10.5772/intechopen.98283 raw materials, and in their transformation performed by microorganisms, perfectly adapted to grow and develop using these raw materials, obtaining, as a result of their microbial activity, fermented products and beverages, with beneficial nutritional and food properties. Fermented food and beverage production industries [9,10], such as yoghurt [11,12], kefir [13,14] and the manufacturing of beer [15] and wine [16], fermented sauces and condiments, such as soy sauce, as well as the production of bio-ethanol [17,18], vinegar [19,20] and some organic acids [21,22], such as citric acid [23] and lactic acid [24], are practical examples of these agroindustries, where microorganisms and enzymes carry out the transformation of raw materials to product.…”