Effects of some metal ions on rainbow trout erythrocytes glutathione S-transferase enzyme: an<i>in vitro</i>study

Çomaklı, Veysel; Çiftçi, Mehmet; Küfrevioğlu, Ömer İrfan

doi:10.3109/14756366.2012.729829

Cited by 17 publications

(16 citation statements)

References 21 publications

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“…In living cells, the deleterious effects of free radicals and their intermediates are eliminated or minimized by various enzymatic and non-enzymatic defense systems. Enzymatic defense is provided by several enzymes such as glutathione S -transferase (GST), glutathione reductase, glutathione peroxidase, superoxide dismutase, and catalase [ 24 ]. The GSTs (EC 2.5.1.18) are a group of multifunctional enzymes that play an important role in animal metabolism [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Compounds from Terminalia mantaly L. (Combretaceae) Stem Bark Exhibit Potent Inhibition against Some Pathogenic Yeasts and Enzymes of Metabolic Significance

et al. 2017

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Background: Pathogenic yeasts resistance to current drugs emphasizes the need for new, safe, and cost-effective drugs. Also, new inhibitors are needed to control the effects of enzymes that are implicated in metabolic dysfunctions such as cancer, obesity, and epilepsy. Methods: The anti-yeast extract from Terminalia mantaly (Combretaceae) was fractionated and the structures of the isolated compounds established by means of spectroscopic analysis and comparison with literature data. Activity was assessed against Candida albicans, C. parapsilosis and C. krusei using the microdilution method, and against four enzymes of metabolic significance: glucose-6-phosphate dehydrogenase, human erythrocyte carbonic anhydrase I and II, and glutathione S-transferase. Results: Seven compounds, 3,3′-di-O-methylellagic acid 4′-O-α-rhamnopyranoside; 3-O-methylellagic acid; arjungenin or 2,3,19,23-tetrahydroxyolean-12-en-28-oïc acid; arjunglucoside or 2,3,19,23-tetrahydroxyolean-12-en-28-oïc acid glucopyranoside; 2α,3α,24-trihydroxyolean-11,13(18)-dien-28-oïc acid; stigmasterol; and stigmasterol 3-O-β-d-glucopyranoside were isolated from the extract. Among those, 3,3′-di-O-methylellagic acid 4′-O-α-rhamnopyranoside, 3-O-methylellagic acid, and arjunglucoside showed anti-yeast activity comparable to that of reference fluconazole with minimal inhibitory concentrations (MIC) below 32 µg/mL. Besides, Arjunglucoside potently inhibited the tested enzymes with 50% inhibitory concentrations (IC50) below 4 µM and inhibitory constant (Ki) <3 µM. Conclusions: The results achieved indicate that further SAR studies will likely identify potent hit derivatives that should subsequently enter the drug development pipeline.

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Section: Introductionmentioning

confidence: 99%

Compounds from Terminalia mantaly L. (Combretaceae) Stem Bark Exhibit Potent Inhibition against Some Pathogenic Yeasts and Enzymes of Metabolic Significance

et al. 2017

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“…30 While it was reported in a different study that Ag + , Cu 2+ , and Hg 2+ ions had an inhibitory effect on glutathione S-transferase enzyme purified from turkey liver, 31 it was reported in another study that Ag + , Cd 2+ , Cr 2+ , and Mg 2+ ions had a noncompetitive inhibitory effect on rainbow trout glutathione S-transferase enzyme. 32 Considering previous studies, it is thought that previously mentioned types of heavy metal ions can also inhibit enzymes. ions had no effect on enzyme activity (Table 3; Figure 7b).…”

Section: −24mentioning

confidence: 99%

Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity

Temel¹,

Çiftçi²

2017

Turk J Chem

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Abstract:The thioredoxin system, found in all living creatures, consists of thioredoxin protein (Trx), thioredoxin reductase enzyme (TrxR), and NADPH. In this study, turkey liver mitochondrial TrxR enzyme with 3.07 EU × mg −1 specific activity was purified 990-fold in a yield of 2.05% using 2',5'-ADP Sepharose 4B affinity chromatography. The purity of the enzyme was measured and the molecular weight of its subunits was determined to be 45.5 kDa by SDS-PAGE. The molecular mass of the enzyme's natural state was found to be 88 kDa using Sephadex-G 150 gel filtration chromatography. In addition, characteristic and kinetic properties of the enzyme were determined. ion increased enzyme activity, and Ni 2+ and Co 2+ ions had no effect on enzyme activity.

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“…We found that the inhibitory effect of the Cu 2 + ion on the GST enzyme activity in both tissues was close. When the results of this study are considered along with those of others (Demirdag et al 2011;Akkemik et al 2012;Comakli et al 2013), it is clear that heavy metals are potential inhibitors of the activity of metabolic enzymes.…”

Section: Discussionmentioning

confidence: 82%

Characterization and Purification of Glutathione S‐Transferase from the Liver and Gill Tissues of Ağrı Balık Lake Trout Salmo trutta labrax and the Effects of Heavy Metal Ions on Its Activity

2015

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Glutathione S-transferase (GST) from the liver and gill tissues of Ağrı Balık Lake Trout (also known as Black Sea Trout) Salmo trutta labrax was characterized and purified, and the toxic effects of some heavy metal ions on the enzyme's activity were analyzed. Liver GST was purified 930 times, resulting in 56% yield using glutathione-agarose affinity chromatography and a specific activity of 60.87 endotoxin units (EU)/mg protein. Using the same procedure, gill GST was purified 576 times, resulting in a 60% yield and specific activity of 46.8 EU/mg protein. The purity check of the purified enzymes was determined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Optimal pH, ionic strength, and stable pH were found for each tissue, and separate KM and Vmax values were determined for reduced glutathione and 2,4-dinitrochlorobenzene substrates. Heavy metal ions that have toxic effects on living organisms and are known to contribute to environmental pollution were selected, and their in vitro effects on enzyme activity were analyzed. The IC50 values and Ki constants of those metal ions showing an inhibitory effect on GST activity were determined.

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Effects of some metal ions on rainbow trout erythrocytes glutathione S-transferase enzyme: anin vitrostudy

Cited by 17 publications

References 21 publications

Compounds from Terminalia mantaly L. (Combretaceae) Stem Bark Exhibit Potent Inhibition against Some Pathogenic Yeasts and Enzymes of Metabolic Significance

Compounds from Terminalia mantaly L. (Combretaceae) Stem Bark Exhibit Potent Inhibition against Some Pathogenic Yeasts and Enzymes of Metabolic Significance

Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity

Characterization and Purification of Glutathione S‐Transferase from the Liver and Gill Tissues of Ağrı Balık Lake Trout Salmo trutta labrax and the Effects of Heavy Metal Ions on Its Activity

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