Effects of Some Insecticides (Deltamethrin and Malathion) and Lemongrass Oil on Fruit Fly (Drosophila melanogaster)

Aljedani, Dalal Musleh

doi:10.3923/pjbs.2021.477.491

Cited by 6 publications

(3 citation statements)

References 32 publications

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“…Some essential oil components of lemongrass are reported to be lethal to insects, probably through their ability to penetrate the system of insects via the respiratory tract or through direct contact with the body of the insects [ 12 ] to target specific enzymes of the insect's nervous system [ 13 ]. Lemongrass has been shown to have the insecticidal and insect-repellent ability towards several insects, including mosquitoes [ 14 ], houseflies [ 15 ], sand flies [ 16 ] and fruit flies ( Drosophila melanogaster ) [ 17 ], hence, it might be a source of effective and non-toxic natural insecticides. Therefore, using Drosophila melanogaster as a model insect, we investigated the insecticidal and AChE inhibitory potentials of Cymbopogon citratus and its bioactive compounds through in vivo and in silico approaches.…”

Section: Introductionmentioning

confidence: 99%

Biochemical evaluation and molecular docking assessment of Cymbopogon citratus as a natural source of acetylcholine esterase (AChE)- targeting insecticides

Johnson¹,

Ojo²,

Ikiriko³

et al. 2021

Biochemistry and Biophysics Reports

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Acetylcholinesterase (AChE) has been an effective target for insecticide development which is a very important aspect of the global fight against insect-borne diseases. The drastic reduction in the sensitivity of insects to AChE-targeting insecticides like organophosphates and carbamates have increased the need for insecticides of natural origin. In this study, we used Drosophila melanogaster as a model to investigate the insecticidal and AChE inhibitory potentials of Cymbopogon citratus and its bioactive compounds. Flies were exposed to 100 and 200 mg/mL C . citratus leaf extract for a 3-h survival assay followed by 45 min exposure for negative geotaxis and biochemical assays. Molecular docking analysis of 45 bioactive compounds of the plant was conducted against Drosophila melanogaster AChE (DmAChE). Exposure to C. citratus significantly reduced the survival rate of flies throughout the exposure period and this was accompanied by a significant decrease in percentage negative geotaxis, AChE activity, catalase activity, total thiol level and a significant increase in glutathione-S-transferase (GST) activity. The bioactive compounds of C. citratus showed varying levels of binding affinities for the enzyme. (+)-Cymbodiacetal scored highest (−9.407 kcal/mol) followed by proximadiol (−8.253 kcal/mol), geranylacetone (−8.177 kcal/mol), and rutin (−8.148 kcal/mol). The four compounds occupied the same binding pocket and interacted with important active site amino acid residues as the co-crystallized ligand (1qon). These compounds could be responsible for the insecticidal and AChE inhibitory potentials of C. citratus and they could be further explored in the development of AChE-targeting insecticides.

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Section: Introductionmentioning

confidence: 99%

Biochemical evaluation and molecular docking assessment of Cymbopogon citratus as a natural source of acetylcholine esterase (AChE)- targeting insecticides

Johnson¹,

Ojo²,

Ikiriko³

et al. 2021

Biochemistry and Biophysics Reports

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“…The continuous use of pesticides in the ecosystem is of great concern, as some of them are highly stable and impact non-target organisms. Therefore, the effects of various pesticide concentrations (Deltamethrin and Malathion) and natural items were studied, according to the findings of a study by Aljedani, [39] lemongrass oil at a concentration (LC 50 = 9.7478 mg LG1) causes half of the Fruit Fly (Drosophila melanogaster) to perish. Additionally, lemongrass oil (LT 50 = 819.745) took 819.745 h to kill half of the test subjects, leading researchers to conclude that the lemon plant and its constituents have excellent potential for use in the management of Drosophila melanogaster, which played a significant role in biological control.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Some Plant Extracts Effectiveness on the Termites Reticulitermes spp. (Isoptera: <i>Rhinotermitidae</i>)

Aljedani¹

2023

Pol. J. Environ. Stud.

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Termites are a major problem in both urban and rural locations around the world because they cause significant damage to vegetation, crops, and wood structures, and cause financial loss [1]. Termites as social insects comprise a king, queen, workers, and soldiers in the colony. Termites can be found in wood, above the floor, and underground. Currently, 2,500 species of termites are known to the world, out of 300 species are acknowledged as a pest [2]. Nevertheless, termites are a widespread, ancient group

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“…Other studies point to relevant aspects of the toxicity mechanism, reinforcing the relevance of this alternative model for the biological sciences and health. Rotenone mediated developmental toxicity in Drosophila melanogaster [88] 2022 Characterization of a novel pesticide transporter and P-glycoprotein orthologues in Drosophila melanogaster [89] 2022 Age-related tolerance to paraquat-induced parkinsonism in Drosophila melanogaster [90] 2022 Potentiation of paraquat toxicity by inhibition of the antioxidant defenses and protective effect of the natural antioxidant, 4-hydroxyisopthalic acid in Drosophila melanogaster [91] 2022 Herbicide Roundup shows toxic effects in nontarget organism Drosophila [92] 2022 Protective capacity of carotenoid trans-astaxanthin in rotenone-induced toxicity in Drosophila melanogaster [93] 2022 Cyromazine Effects the Reproduction of Drosophila by Decreasing the Number of Germ Cells in the Female Adult Ovary [94] 2022 Low doses of the organic insecticide spinosad trigger lysosomal defects, elevated ROS, lipid dysregulation, and neurodegeneration in flies [95] 2022 Short exposure to nitenpyram pesticide induces effects on reproduction, development and metabolic gene expression profiles in Drosophila melanogaster (Diptera: Drosophilidae) [96] 2022 Using tissue specific P450 expression in Drosophila melanogaster larvae to understand the spatial distribution of pesticide metabolism in feeding assays [97] 2021 An integrated host-microbiome response to atrazine exposure mediates toxicity in Drosophila [98] 2021 Effects of some insecticides (deltamethrin and malathion) and lemongrass oil on fruit fly (Drosophila melanogaster) [99] 2021 Chronic exposure to paraquat induces alpha-synuclein pathogenic modifications in Drosophila [100] 2021 Pre-imaginal exposure to Oberon® disrupts fatty acid composition, cuticular hydrocarbon profile and sexual behavior in Drosophila melanogaster adults [101] 2021 Transcriptomic identification and characterization of genes commonly responding to sublethal concentrations of six different insecticides in the common fruit fly, Drosophila melanogaster [102] 2021 Protective effect of Catharanthus roseus plant extracts against endosulfan and its isomers induced impacts on non-targeted insect model, Drosophila melanogaster and live brain cell imaging [103] 2021 Chlordane exposure causes developmental delay and metabolic disorders in Drosophila melanogaster [104] 2021 Dietary behavior of Drosophila melanogaster fed with genetically-modified corn or Roundup ® [105] Brazilian Archives of Biology and Technology. Vol.67: e24230091, 2024 www.scielo.br/babt Cont.…”

Section: The Alternative Animal Model Drosophila Melanogaster As a Bi...mentioning

confidence: 99%

Drosophila melanogaster as a Biotechnological Tool to Investigate the Close Connection Between Fatty Diseases and Pesticides

de Oliveira-Júnior,

Oliveira,

Pansa

et al. 2024

Braz. arch. biol. technol.

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Nonalcoholic fatty liver disease (NAFLD) is a public health problem developed by different etiologies, which induces metabolic dysfunctions and triglycerides accumulation in hepatocytes. This lipid accumulation can generate lipotoxicity, inflammation and the production of reactive oxygen species, collaborating with the progression of liver pathogenesis to more deleterious stage. Among the elements that initiate the establishment of liver diseases, pesticides should be considered. Worldwide, the use of agricultural chemicals to increase food production may accumulate in the environment, affecting non-target organisms. Thus, worldwide legislation must control pesticides use to preserve economies and lives. In this context, to address pesticide toxicity, the alternative animal model, Drosophila melanogaster, emerges as relevant biotechnological tool to investigate molecular connectors of toxic mechanisms in the establishment and development of NAFLD and liver diseases. In this review a comprehensive explanation about pesticides on human health and the use of Drosophila melanogaster as an alternative approach to defeat NAFLD will be presented.

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Effects of Some Insecticides (Deltamethrin and Malathion) and Lemongrass Oil on Fruit Fly (Drosophila melanogaster)

Cited by 6 publications

References 32 publications

Biochemical evaluation and molecular docking assessment of Cymbopogon citratus as a natural source of acetylcholine esterase (AChE)- targeting insecticides

Biochemical evaluation and molecular docking assessment of Cymbopogon citratus as a natural source of acetylcholine esterase (AChE)- targeting insecticides

Evaluation of Some Plant Extracts Effectiveness on the Termites Reticulitermes spp. (Isoptera: <i>Rhinotermitidae</i>)

Drosophila melanogaster as a Biotechnological Tool to Investigate the Close Connection Between Fatty Diseases and Pesticides

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