Effects of Some Antibiotics on Paraoxonase from Human Serum in Vitro and from Mouse Serum and Liver in Vivo

Antidepressant and antipsychotic drugs differentially affect PON1 enzyme activity

“…When the same antibiotics were given in vivo to mice, however, increases or decreases of serum PON1 activities at different time-points were observed, with similar results observed in the liver. It has also been reported that mouse liver PON activity decreased with some contraceptives while the serum PON activity increased 15,38 . The effect of different pharmaceutical drugs on paraoxonase enzyme activity can be used to clarify PON1 status in the metabolism.…”

Differential effects of some antibiotics on paraoxonase enzyme activity on human hepatoma cells (HepG2) in vitro

“…It is also reported that sodium ampicillin, ciprofloxacin and clindamycin phosphate significantly inhibited purified human serum PON1 activity in a dosedependent fashion. These antibiotics also showed different inhibition effects on mouse serum and liver [10].…”

“…Paraoxonase should be studied more given its physiological and metabolic importance on medically important drugs that are commonly used in therapies such as antibiotics. There are only a few reports that have determined the changes in paraoxonase enzyme activities by antibiotics [9,10,11]. In our previous study, we reported that different classes of antibiotics differentially affect the PON1 enzyme activity from mouse liver, mouse serum and human serum in vitro.…”

“…Rifamycins are particularly effective against mycobacteria and are therefore used to treat tuberculosis, leprosy, and Mycobacterium avium complex (MAC) infections [14]. Clindamycin is a lincosamide antibiotic and it prevents the protein synthesis of bacteria, causing the cells to die [10] (Figure 1). …”

In vitro efficacy of some cattle drugs on bovine serum paraoxonase 1 (PON1) activity

“…So, the drug must be used carefully and the dosage closely monitored to decrease side effects. In addition, Sinan et al 53 performed some experiments about the in vitro effects of sodium ampicillin, ciprofloxacin, and clindamycin phosphate on PON1 purified from human serum. The study found that sodium ampicillin, ciprofloxacin, and clindamycin phosphate were potent inhibitors for human serum PON1, and IC 50 values were 27.51 mM, 0.313 mM and 0.902 mM, respectively.…”