21Soil-borne pathogen Setophoma terrestris is the causal agent of pink root of onion, one 22 of the most challenging diseases in onion production. Conventional approaches for 23 managing the disease like solarization, soil fumigation and crop rotation have not been 24 proven effective enough. In this work, we evaluated the biocontrol capacity of Bacillus 25 subtilis ALBA01 (BsA01) against S. terrestris, in a highly susceptible onion cultivar, both 26 under greenhouse and field conditions. Disease incidence and severity were evaluated 27 together with growth, photosynthesis among other physiological variables and yield 28 parameters. When compared with plants infected with the pathogen, those plants co-29 inoculated with BsA01 showed significantly less damage and levels of biocontrol above 30 50%. With regard to physiological parameters, plants challenged with S terrestris and 31 inoculated with BsA01 performed as well as the control non-infected plants revealing a 32 growth promotion effect of BsA01 on onion plants.33