Effects of soil management on structure and activity of denitrifying bacterial communities

Pastorelli, Roberta; Landi, Silvia; Trabelsi, Darine; Piccolo, Raimondo; Mengoni, Alessio; Bazzicalupo, Marco; Pagliai, M.

doi:10.1016/j.apsoil.2011.07.002

Cited by 47 publications

(42 citation statements)

References 56 publications

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“…The copy numbers of denitrifying genes, such as nirK and nirS, increase with increasing the water content (Szukics et al, 2010;Di et al, 2014), but nosZ communities exhibit a great stability in response to changes in the soil moisture (Stres et al, 2008). Pastorelli et al (2011) reported that the season did not affect the nosZ-gene-bearing denitrifiers, but we detected a variation of nosZ with the sampling time. Of the measured soil chemical properties, the soil SOC, pH and C:N were significantly correlated with the copy numbers of the nosZ gene.…”

Section: Temporal Changes In the Abundance Of Denitrifiers Under Diffcontrasting

confidence: 37%

不同肥料处理下茶园土壤细菌和古菌群落的时间变化研究

Wang

Yang

et al. 2014

J. Zhejiang Univ. Sci. B

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Abstract:It is important to understand the effects of temporal changes in microbial communities in the acidic soils of tea orchards with different fertilizers. A field experiment involving organic fertilizer (OF), chemical fertilizer (CF), and unfertilized control (CK) treatments was arranged to analyze the temporal changes in the bacterial and archaeal communities at bimonthly intervals based on the 16S ribosomal RNA (rRNA) gene using terminal restriction fragment length polymorphism (T-RFLP) profiling. The abundances of total bacteria, total archaea, and selected functional genes (bacterial and archaeal amoA, bacterial narG, nirK, nirS, and nosZ) were determined by quantitative polymerase chain reaction (qPCR). The results indicate that the structures of bacterial and archaeal communities varied significantly with time and fertilization based on changes in the relative abundance of dominant T-RFs. The abundancy of the detected genes changed with time. The total bacteria, total archaea, and archaeal amoA were less abundant in July. The bacterial amoA and denitrifying genes were less abundant in September, except the nirK gene. The OF treatment increased the abundance of the observed genes, while the CF treatment had little influence on them. The soil temperature significantly affected the bacterial and archaeal community structures. The soil moisture was significantly correlated with the abundance of denitrifying genes. Of the soil chemical properties, soil organic carbon was the most important factor and was significantly correlated with the abundance of the detected genes, except the nirK gene. Overall, this study demonstrated the effects of both temporal alteration and organic fertilizer on the structures of microbial communities and the abundance of genes involved in the nitrogen cycle.

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Section: Temporal Changes In the Abundance Of Denitrifiers Under Diffcontrasting

confidence: 37%

不同肥料处理下茶园土壤细菌和古菌群落的时间变化研究

Wang

Yang

et al. 2014

J. Zhejiang Univ. Sci. B

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“…The boundary value of redox potential for the emission of N 2 O was about +250 mV and about 200 mV for consumption thereof under hypoxic conditions. Pastorelli et al (2011) found that the influx of C sources and energy into the oligotrophic soil system is a major driving force in biogeochemical cycles. There are several lines of evidence to support the proposal that severe drought augments dissolved organic carbon (DOC) production and thus controls the observed increases in DOC concentration (Worrall and Burt, 2008).…”

Section: Discussionmentioning

confidence: 99%

Effect of Long Storage and Soil Type on the Actual Denitrification and Denitrification Capacity to N2O Formation

Włodarczyk¹,

Szarlip²,

Kozieł³

et al. 2014

International Agrophysics

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A b s t r a c t. The actual denitrification to N 2 O and denitrification capacity to N 2 O after flooding of different soil samples stored for over 25 years in air-dry conditions and fresh, air dried samples were compared in our study. Zero N 2 O release was observed from the stored soils but the fresh soil samples had very low actual denitrification to N 2 O. NO 3 -addition significantly increased the amount of N 2 O (denitrification capacity to N 2 O) released after flooding, which depended on the length of storage and type of soils and was much higher in stored soils. Prolonged exposure of the soils to drought conditions caused a greater decrease in the Eh value compared with the fresh soil. The total cumulative release of N 2 O from the stored and fresh soils was correlated with the reduced NO 3 -and organic C content in soils enriched with NO 3 -. Some soils showed the capability of N 2 O consumption. CO 2 release depended on the length of storage and type of soils under flooding after prolonged drought. On average, CO 2 release was higher from the stored rather than fresh soils. The organic C content in the stored soils was generally lower than in the fresh soils, probably due to the storage effect. The cumulative CO 2 release from the stored soils was well correlated with the organic C while no correlation was observed for the fresh soil samples.K e y w o r d s: actual denitrification to N 2 O, denitrification capacity to N 2 O, long-and very short-storage time, soil respiration, archived soil INTRODUCTIONBiological activity in soil can be represented by several different parameters such as respiration, enzyme activity, ammonification, nitrification, denitrification, and emission of gaseous metabolites as well as oxidation-reduction processes (Bieganowski et al., 2013;W³odarczyk et al., 2011).Soils are subjected to temporal variations in temperature and moisture that can cause changes in physicochemical properties. Soil dry/wet cycles result from natural variations in soil moisture driven by environmental and biophysical processes such as precipitation, evapotranspiration, and drainage. Management factors such as irrigation, tillage and land cover (ie. vegetation type) can moderate or accentuate the amplitude of these natural cycles (Oliveira et al., 2005).Under in situ conditions, denitrification rates depend on oxygen availability, soil moisture, soil type, pH, NO 3 -concentration, but also on the availability of labile carbon compounds in soil (Burford and Bremner, 1975; Senbayram et al., 2009). Nitrate (NO 3 -) is a key node in the network of the assimilatory and respiratory nitrogen pathways. For bacteria, it is both a nitrogen source and an electron acceptor (Hayatsu et al., 2008). In agriculture and wastewater treatment, NO 3 -respiration by microorganisms is an important process in respect to economics, greenhouse gas emission, and public health. Several microbial processes compete for NO

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“…The addition of the fermented manure in the stabilized soil C:N ratio (7.9:1) to the rest of the treatments (with and without the CL and Ca-additions) modified the denitrification rate and the N 2 O-releasing indicated a similar response as the 1-order reaction and was non-linear (logarithmic increase of N 2 O at the time). Pastorelli et al (2011) found that the influx of C sources and energy into the oligotrophic soil system was a major driving force in biogeochemical cycles.…”

Section: Incomplete Denitrification (Ad-n 2 O) and Complete Denitrifimentioning

confidence: 99%

“…This directly resulted, amongst other effects, in the diversity of soil pH and the availability of substrates, and indirectly resulted in the type of microbial community and the biochemical reaction. Pastorelli et al (2010Pastorelli et al ( , 2011 have shown that the extent and degree of active bacterial diversity and the microbial communities in soil are clearly affected by the type of management and agriculture practices. The results indicated that the tested soil showed the highest N 2 O-RA in a weakly acidic reaction (M-treatment) but much lower in an acid reaction (c-soil).…”

Section: N 2 O-reducing Activity (N 2 O/n 2 +N 2 O Ratio) Under Floodingmentioning

confidence: 99%

N2O-reducing activity of soil amended with organic and inorganic enrichments under flooded conditions

Ksiezopolska

Włodarczyk

Brzezińska

et al. 2017

Sci. agric. (Piracicaba, Braz.)

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Effects of soil management on structure and activity of denitrifying bacterial communities

Cited by 47 publications

References 56 publications

不同肥料处理下茶园土壤细菌和古菌群落的时间变化研究

不同肥料处理下茶园土壤细菌和古菌群落的时间变化研究

Effect of Long Storage and Soil Type on the Actual Denitrification and Denitrification Capacity to N2O Formation

N2O-reducing activity of soil amended with organic and inorganic enrichments under flooded conditions

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