Effects of Soft Denture Liners on L929 Fibroblasts, HaCaT Keratinocytes, and RAW 264.7 Macrophages

Abstract

The effects of six soft liners (Ufi Gel P (UG), Sofreliner S (SR), Durabase Soft (D), Trusoft (T), Coe Comfort (CC), and Softone (ST)) on L929, HaCat, and RAW 264.7 cells were investigated. Eluates (24 and 48 h) from the materials were applied on the cells and the viability, type of cell death, and morphology were evaluated. Cells were also seeded on the specimens' surfaces (direct contact) and incubated (24 or 48 h), and viability was analyzed. Controls were cells in culture medium without eluates or specimens. For cell viability, no significant differences were found among materials or between extraction periods, and the liners were noncytotoxic or slightly cytotoxic. Morphology of RAW 264.7 cells was altered by the 24 h eluates from CC and D and the 48 h eluates from SR, CC, and D. The 24 and 48 h eluates from all materials (except T) increased the percentages of L929 necrotic cells. For direct contact tests, the lowest cytotoxicity was observed for UG and SR. Although eluates did not reduce viability, morphology alterations and increase in necrosis were seen. Moreover, in the direct contact, effects on viability were more pronounced, particularly for D, T, CC and ST. Thus, the use of UG and SR might reduce the risk of adverse effects.

Surface Characterization, Biocompatibility and Antifungal Efficacy of a Denture-Lining Material Containing Cnidium officinale Extracts

“…The biological properties of the denture-lining materials are also important for clinical application [ 6 ]. As denture-lining materials are in direct contact with the oral mucosa, investigating the cytotoxicity of the denture-lining material is important [ 4 , 6 , 19 ]. The in vitro biological evaluation of clinical materials is exceedingly crucial [ 20 ].…”

Mouthwash with Active Oxygen (blue<sup>®</sup>m) Induces Keratinocytes Proliferation

“…Other in vitro studies [2] [28], proliferation in these cases is analyzed indirectly by readings of products derived from cellular metabolism (MTT, Alamar Blue, etc. ), therefore, they do not reflect proliferation properly, as cell activity varies greatly during the cell life cycle.…”

Space Maintainers Used in Pediatric Dentistry: An Insight of Their Biosecurity Profile by Applying In Vitro Methods

“…The in vitro methods employed in the current study have already proved to be relevant for offering preliminary data in establishing the biological profile of different dental materials [17][18][19], whereas keratinocyte cultures proved to be good candidates as in vitro models for providing the preliminary biosecurity of different products related to oral use [20][21][22].…”