Effects of Sodium Trimetaphosphate, Associated or Not with Fluoride, on the Composition and pH of Mixed Biofilms, before and after Exposure to Sucrose

Abstract

The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose. The biofilms received three treatments (72, 78, and 96 h after the beginning of their formation), at three TMP concentrations (0.25, 0.5, or 1%), with or without F at 500 ppm. Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also tested as controls. Biofilm pH was measured and the concentrations of F, Ca, and P were determined (solid and fluid phases). In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined. The data were submitted by two-way ANOVA, followed by Fisher’s least significant difference test (p < 0.05). Treatment with TMP and 500 ppm F led to higher F concentration in the biofilm fluid. Although TMP did not affect Ca concentrations, biofilms treated with TMP alone presented higher P concentrations. Treatment with 1% TMP and F led to the highest pH values of the biofilm, both before and after the cariogenic challenge. It was concluded that TMP increases F and P in the biofilm and that its presence promotes an increase in the pH of the medium, even after the cariogenic challenge.

Effects of Sodium Hexametaphosphate and Fluoride on the pH and Inorganic Components of Streptococcus mutans and Candida albicans Biofilm after Sucrose Exposure

“…The biofilms were treated 72, 78, and 96 h after the beginning of the biofilm formation, for 1 min [ 30 , 31 ]. The two first treatments were performed in order to achieve and verify the cumulative effect of the compounds, similarly to what happens in in vivo conditions after brushing with a fluoridated dentifrice.…”

“…The two first treatments were performed in order to achieve and verify the cumulative effect of the compounds, similarly to what happens in in vivo conditions after brushing with a fluoridated dentifrice. Regarding the last treatment, it was performed in order to evaluate the effect of the compounds on the biofilms right after the treatment [ 30 , 31 ].…”

In it together: Candida–bacterial oral biofilms and therapeutic strategies

“…There also have been attempts to combine synthetic compounds and a classic anticaries agent such as fluoride. Sodium trimetaphosphate accompanied with fluoride elevated pH of C. albicans-S. mutans biofilm close to the neutral values, which may reduce the tooth demineralization (Cavazana et al, 2020). The combinations of ttfarnesol and 4 0 -hydroxychalcone were effective against preformed C. albicans-S. mutans biofilm when combined with fluoride (Lobo et al, 2021).…”

“…albicans–S . mutans biofilm close to the neutral values, which may reduce the tooth demineralization (Cavazana et al ., 2020). The combinations of tt‐farnesol and 4′‐hydroxychalcone were effective against preformed C .…”

Multiclawed SiO2 Nano-Antibacterial Agent Based on Charge Inversed Ce6 Ionic Liquid Polymers for Combating Oral Biofilm Infection

“…Because oral biofilm develop when microbes accumulate and form structured communities encapsulated within an extracellular matrix such as exopolysaccharides (EPS) [7,8], the sugars are fermented by bacteria within the EPS matrix, then created highly acidic microenvironments and negative charge. The pH values often reach pH~4.5 or even lower in oral biofilm and particularly after exposure to sucrose, starch, and other cariogenic food products [9]. So, photosensitizer delivery system that could response to lower pH microenvironments is an effective method to improve delivery efficiency.…”