Effects of Sodium Silicate Complex against Hemorrhagic Activities Induced by Protobothrops mucrosquamatus Venom

Chen, Yen-Chia; Wang, Tse-Yao; Huang, Yung-Cheng; Chang, Kun-Che; Chen, Minhui; Liu, Chien-Chun; Liu, Kuei-Lin; Yang, Ya-Sung; Yen, David Hung-Tsang; Fan, Ju-Sing

doi:10.3390/toxins13010059

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…Vol www.nature.com/scientificreports/ Until recently, the assessment of snake venom haemorrhage was faced with the same issues. Therefore, we first developed a simple computational tool to improve accuracy of quantifying haemorrhage severity, which has since been implemented by different groups within the field [23][24][25][26][27][28][29][30][31] . This encouraged us to build a more sophisticated machine learning guided tool that further increased the accuracy, accessibility, and reproducibility of snake venom haemorrhage assessment 32 .…”

Section: Discussionmentioning

confidence: 99%

Machine-learning guided Venom Induced Dermonecrosis Analysis tooL: VIDAL

Laprade

Bartlett

Christensen

et al. 2023

Preprint

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Snakebite envenoming is a global public health issue that causes significant morbidity and mortality, particularly in low-income regions of the world. The clinical manifestations of envenomings vary depending on the snake's venom, with paralysis, haemorrhage, and necrosis being the most common and medically relevant effects. To assess the efficacy of antivenoms against dermonecrosis, a preclinical testing approach involves in vivo mouse models that mimic local tissue effects of cytotoxic snakebites in humans. However, current methods for assessing necrosis severity are time-consuming and susceptible to human error. To address this, we present the Venom Induced Dermonecrosis Analysis tool (VIDAL), a machine-learning-guided image-based solution that can automatically identify dermonecrotic lesions in mice, adjust for lighting biases, scale the image, extract lesion area and discolouration, and calculate the severity of dermonecrosis. We also introduce a new unit, the dermonecrotic unit (DnU), to better capture the complexity of dermonecrosis severity. Our tool is comparable to the performance of state-of-the-art histopathological analysis, making it an accessible, accurate, and reproducible method for assessing dermonecrosis. Given the urgent need to address the neglected tropical disease that is snakebite, high-throughput technologies such as VIDAL are crucial in developing and validating new and existing therapeutics for this debilitating disease.

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Section: Discussionmentioning

confidence: 99%

Machine-learning guided Venom Induced Dermonecrosis Analysis tooL: VIDAL

Laprade

Bartlett

Christensen

et al. 2023

Preprint

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“…In our earlier study, we were able to improve the classical rodent skin test by increasing the accuracy of haemorrhage characterisation and reducing the time required for analysis in comparison to the original approach, as well establish that HaUs accurately reflect haemoglobin content analysis 18 . Therefore, our previous tool has been used in a series of later publications [28][29][30][31][32][33][34][35][36][37]. Nevertheless, our prior approach still remained time consuming, and required familiarisation with a new software and access to a colour pantone.…”

Section: Discussionmentioning

confidence: 99%

ALOHA: AI-guided tool for the quantification of venom-induced haemorrhage in mice

Jenkins

Laprade

Sánchez

et al. 2022

Preprint

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Venom-induced haemorrhage constitutes a severe pathology in snakebite envenomings, especially those inflicted by viperid species. In order to both explore venom compositions accurately, and evaluate the efficacy of viperid antivenoms for the neutralisation of haemorrhagic activity it is essential to have available a precise, quantitative tool for empirically determining venom-induced haemorrhage. Thus, we have built on our prior approach and developed a new AI-guided tool (ALOHA) for the quantification of venom-induced haemorrhage in mice. Using a smartphone, it takes less than a minute to take a photo, upload the image, and receive accurate information on the magnitude of a venom-induced haemorrhagic lesion in mice. This substantially decreases analysis time, reduces human error, and does not require expert haemorrhage analysis skills. Furthermore, its open access web-based graphical user interface makes it easy to use and implement in laboratories across the globe. Together, this will reduce the resources required to preclinically assess and control the quality of antivenoms, whilst also expediting the profiling of hemorrhagic activity in venoms for the wider toxinology community.

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“…Moreover, excessive administration of EDTA may cause electrolyte imbalance in patients. A previous study showed that a premix of sodium silicate complex (SSC) with PM could reduce subdermal hemorrhage and elongate the survival in a rodent model of PM envenoming [ 17 ], suggesting that SSC may not only inhibit hemotoxic effects but also alleviate neurotoxic influences. Thus, SSC could be an adjuvant as first aid for viper snakebites.…”

Section: Treatmentmentioning

confidence: 99%

“…Therefore, there remains an unmet need to develop an adjuvant therapy for snakebite envenoming. Most viper-bite cases studied, in both clinical reports and animal studies, were reported and diagnosed with hemorrhage and tissue necrosis in skin and muscle [ 15 , 16 , 17 ]. Little is known about viper-bite-induced complications in the central nervous system (CNS).…”

Section: Introductionmentioning

confidence: 99%

Cerebral Complications of Snakebite Envenoming: Case Studies

Lin

Chen

Liu

et al. 2022

Toxins

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There are an estimated 5.4 million snakebite cases every year. People with snakebite envenoming suffer from severe complications, or even death. Although some review articles cover several topics of snakebite envenoming, a review of the cases regarding cerebral complications, especially rare syndromes, is lacking. Here, we overview 35 cases of snakebite by front-fanged snakes, including Bothrops, Daboia, Cerastes, Deinagkistrodon, Trimeresurus, and Crotalus in the Viperidae family; Bungarus and Naja in the Elapidae family, and Homoroselaps (rare cases) in the Lamprophiidae family. We also review three rare cases of snakebite by rear-fanged snakes, including Oxybelis and Leptodeira in the Colubridae family. In the cases of viper bites, most patients (17/24) were diagnosed with ischemic stroke and intracranial hemorrhage, leading to six deaths. We then discuss the potential underlying molecular mechanisms that cause these complications. In cases of elapid bites, neural, cardiac, and ophthalmic disorders are the main complications. Due to the small amount of venom injection and the inability to deep bite, all the rear-fanged snakebites did not develop any severe complications. To date, antivenom (AV) is the most effective therapy for snakebite envenoming. In the six cases of viper and elapid bites that did not receive AV, three cases (two by viper and one by elapid) resulted in death. This indicates that AV treatment is the key to survival after a venomous snakebite. Lastly, we also discuss several studies of therapeutic agents against snakebite-envenoming-induced complications, which could be potential adjuvants along with AV treatment. This article organizes the diagnosis of hemotoxic and neurotoxic envenoming, which may help ER doctors determine the treatment for unidentified snakebite.

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Effects of Sodium Silicate Complex against Hemorrhagic Activities Induced by Protobothrops mucrosquamatus Venom

Cited by 5 publications

References 38 publications

Machine-learning guided Venom Induced Dermonecrosis Analysis tooL: VIDAL

Machine-learning guided Venom Induced Dermonecrosis Analysis tooL: VIDAL

ALOHA: AI-guided tool for the quantification of venom-induced haemorrhage in mice

Cerebral Complications of Snakebite Envenoming: Case Studies

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