Effects of sodium houttuyfonate on transcriptome of Pseudomonas aeruginosa

Abstract

ObjectivesThe purpose of this experiment is to analyze the changes of transcriptome in Pseudomonas aeruginosa under the action of sodium houttuyfonate (SH) to reveal the possible mechanism of SH inhibiting P. aeruginosa. We analyzed these data in order to compare the transcriptomic differences of P. aeruginosa in SH treatment and blank control groups.Data descriptionIn this project, RNA-seq of BGISEQ-500 platform was used to sequence the transcriptome of P. aeruginosa, and sequencing data of 8 samples of P. aeruginosa are generated as follows: SH treatment (SH1, SH2, SH3, SH4), negative control (Control 1, Control 2, Control 3, Control 4). Quality control is carried out on raw reads to determine whether the sequencing data is suitable for subsequent analysis. Totally 170.53 MB of transcriptome sequencing data is obtained. Then the filtered clean reads are aligned and compared to the reference genome to proceed second quality control. After completion, 5938 genes are assembled from sequencing data. Further quantitative analysis of genes and screening of differentially expressed genes based on gene expression level reveals that there are 2047 significantly differentially expressed genes under SH treatment, including 368 up-regulated genes and 1679 down-regulated genes.

Sodium New Houttuyfonate Inhibits Candida albicans Biofilm Formation by Inhibiting the Ras1-cAMP-Efg1 Pathway Revealed by RNA-seq

“…The larvae were observed continuously for 84 h and the number of deaths was recorded to determine the survival rate of each group. In view of studies that show that the LD 50 of SNH is 119.14 mg/kg ( Jiang et al, 2006 ) and the LD 100 of SNH is 200 mg/kg ( Lou et al, 2012 ), our research group selected the dose range 1024–32 μg/mL for SH in previous experiments ( Wang et al, 2019 ; Zhao et al, 2019 ). Therefore, the safety range of SNH is large, and it has no obvious cytotoxicity.…”

Alkyl ferulic acid esters: Evaluating their structure and antibacterial properties

“…Floating motion plays an important role in the pathogenic ability of P. aerugino, which can initiate the adhesion of bacteria to the adhesive surface and contribute to the formation of P. aerugino biofilm. Therefore, these partial results suggest that the inhibition of this motion may be one of the reasons for the reduction of biofilm formation (Diao et al, 2018;Zhao et al, 2019). Furthermore, a recent comparative study by Xu et al (2022) revealed that FA had a greater biofilm repression effect than p-coumaric acid (p-CA) against pathogenic Salmonella enteritidis by binding to proteins involved in flagella motility.…”