Effects of sodium fluoride on the reproductive development of Bombyx mori

Tang, Wenchao; Zheng, Xiaokun; Li, Dan; Xiao, Yuanyuan; Shang, Shan; Shi, Min; Zhu, Yong

doi:10.1016/j.etap.2018.09.009

Cited by 16 publications

(2 citation statements)

References 35 publications

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“…Kidney tissue blocks ($1 mm 3 ) were fixed in 4% glutaraldehyde fixing solution (Sangon Biotech) for 2 h at 4 C. Then washed, post-fixed, dehydrated, polymerized, serial ultrathin sections, stained, and the specific operation steps followed the methods described in our previous publication (Tang et al, 2018). Images were obtained using a TEM (JEM-1400PLUS, JEOL Ltd., Tokyo, Japan) at 100 Kv.…”

Section: Ultrastructure Observation By Transmission Electron Microsco...mentioning

confidence: 99%

Analysis of cantharidin‐induced kidney injury and the protective mechanism of resveratrol in mice determined by liquid chromatography/mass spectrometry‐based metabonomics

Xiao,

Liu,

Zhang

et al. 2024

J of Applied Toxicology

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Cantharidin (CTD) is the main active component in the traditional Chinese medicine Mylabris and an effective anti‐tumor agent. However, it is relatively toxic and exhibits nephrotoxicity, which limits its clinical use. However, its toxic mechanism is not clear. The toxic effects of CTD exposure on the kidney and the protective effect of resveratrol (RES) were studied in a mouse model, by determination of serum biochemical and renal antioxidant indicators, histopathological and ultrastructural observation, and metabonomics. After CTD exposure, serum uric acid, creatinine, and tissue oxidative stress indicators increased, and the renal glomerular and tubular epithelial cells showed clear pathological damage. Ultrastructure observation revealed marked mitochondrial swelling, endoplasmic reticulum dilation, and the presence of autophagy lysosomes in glomerular epithelial cells. RES ameliorated the renal injury induced by CTD. Metabonomics analysis indicated that CTD can induce apoptosis and oxidative damage in kidney cells, mainly by disrupting sphingolipid and glutathione metabolism, increasing sphingosine and sphingomyelin levels, and decreasing glutathione levels. RES counteracts these effects by regulating renal cell proliferation, the inflammatory response, oxidative stress, and apoptosis, by improving the levels of phosphatidylcholine (PC), LysoPC, and lysophosphatidyl glycerol in the glycerophospholipid metabolism pathway, thereby reducing CTD‐induced nephrotoxicity. The mechanisms of CTD‐induced renal injury and the protective effect of RES were revealed by metabonomics, providing a basis for evaluating clinical treatment regimens to reduce CTD‐induced nephrotoxicity.

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Section: Ultrastructure Observation By Transmission Electron Microsco...mentioning

confidence: 99%

Analysis of cantharidin‐induced kidney injury and the protective mechanism of resveratrol in mice determined by liquid chromatography/mass spectrometry‐based metabonomics

Xiao,

Liu,

Zhang

et al. 2024

J of Applied Toxicology

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“…It has been widely used in functional gene research [10,11], metabolic pathway and mechanism analysis [12], and key marker screening [13,14]. There have been reports on the transcriptome [15][16][17], proteome [9], and microbial diversity [5] after NaF stress in silkworm. However, the differences in metabolic pathways of fluoride-tolerant and sensitive silkworm varieties under NaF stress have not been reported using metabonomics.…”

Section: Introductionmentioning

confidence: 99%

Gas Chromatography-Mass Spectrometry Based Midgut Metabolomics Reveals the Metabolic Perturbations under NaF Stress in Bombyx mori

Zhang

Qian

et al. 2019

Insects

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Fluoride tolerance is an important economic trait in sericulture, especially in some industrial development regions. Analyses of physiological changes involving structural damage to the insect body and molecular analyses of some related genes have focused on this area; however, the changes that occur at the metabolic level of silkworms after eating fluoride-contaminated mulberry leaves remain unclear. Here, metabonomic analysis was conducted using gas chromatography-mass spectrometry (GC-MS) to analyze the changes in midgut tissue after NaF stress using silkworm strains 733xin (susceptible stain) and T6 (strain resistant to fluoride), which were previously reported by our laboratory. Differential metabolomics analysis showed that both T6 and 733xin strains displayed complex responses after exposure to 200 mg/kg NaF. The purine metabolism and arginine and proline metabolic pathways of fluoride-tolerant strains reached significant levels, among which 3′-adenylic acid and hypoxanthine were significantly upregulated, whereas guanine, allantoic acid, xanthine, N-acetyl-L-glutamic acid, and pyruvate were significantly downregulated. These metabolic pathways may be related to the fluoride tolerance mechanism of NaF poisoning and tolerant strains.

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