Effects of sodium fluoride on immune response in murine macrophages

Fuente, Beatriz de la; Vázquez, Marta; Rocha, R.A.; Devesa, Vicenta; Vélez, Dinoraz

doi:10.1016/j.tiv.2016.03.001

Cited by 36 publications

(11 citation statements)

References 34 publications

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“…Micromolar fluoride concentrations can affect mitochondrial activity and subsequently alter reactive oxygen species (ROS) production . These changes may lead to a different expression level of pro-inflammatory cytokines . The osteoimmunology has shown that immune cells play a vital role in regulating bone dynamics.…”

Section: Introductionmentioning

confidence: 99%

Sodium Fluoride under Dose Range of 2.4–24 μM, a Promising Osteoimmunomodulatory Agent for Vascularized Bone Formation

Xia

Mai

et al. 2018

ACS Biomater. Sci. Eng.

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Fluoride has essential effects on bone physiological activity and is widely used in bone biomaterials modification. However, this beneficial effect is highly related to the dose range and improper dosing can lead to pathological conditions such as fluorosis of bone. Therefore, this study first investigated the dose dependent effect of fluoride on bone regeneration. In the range of 0.24–240 μM, in vivo vascularized bone formation can be achieved via fine-tuning the fluoride concentration, and the peak osteogenic effect was found at 2.4–24 μM. The underlying mechanism is related to the modulation of the osteoimmune environment. Fluoride elicited significant osteoimmunomodulatory effect in modulation of the inflammatory cytokines and expression of osteogenic factors (BMP2, OSM, spermine/spermidine) and angiogenic factor (VEGF, IGF-1) during the early response. Fluorine with the doses of 2.4 and 24 μM could increase polyamines and IGF-1 production in macrophages, thus promoting osteogenesis of BMSCs and angiogenesis of HUVECs. These doses could also inhibit the inflammatory response of macrophages. In vitro osteogenesis and angiogenesis were both improved by the fluorine (2.4 and 24 μM)/macrophage conditioned medium, which is consistent with the in vivo results. These results collectively imply that fluoride is an effective osteoimmunomodulatory agent that can regulate both osteogenesis and angiogenesis. “Osteoimmune-smart” bone biomaterials can be developed via incorporating fluorine, and the release concentration should be controlled within the range of 2.4–24 μM for improved bone formation.

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Section: Introductionmentioning

confidence: 99%

Sodium Fluoride under Dose Range of 2.4–24 μM, a Promising Osteoimmunomodulatory Agent for Vascularized Bone Formation

Xia

Mai

et al. 2018

ACS Biomater. Sci. Eng.

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“…Yan et al [ 37 ] has also reported that fluoride can induce the expression of TNF-α, IL-1β and interleukin-6 (IL-6) in rat brain. In addition, De la Fuente et al [ 38 ] has found that low levels of fluoride can decrease the expression of the anti-inflammatory cytokine interleukin-10 (IL-10) in murine macrophages. However, the effects of fluoride on NF-κB activation and inflammatory mediators in the kidney of animals and human beings are unclearly at present.…”

Section: Introductionmentioning

confidence: 99%

Sodium fluoride induces renal inflammatory responses by activating NF-κB signaling pathway and reducing anti-inflammatory cytokine expression in mice

et al. 2017

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Fluoride is widely distributed in the environment and often results in adverse health effects on animals and human beings. It has been proved that fluoride can induce inflammatory responses in vitro. However, very limited reports are focused on fluoride-induced inflammatory responses in vivo. In this study, mice were used to investigate sodium fluoride (NaF) induced renal inflammatory responses and the potential mechanism by using the methods of pathology, biochemistry, enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. A total of 240 ICR mice were randomly divided into four equal groups: the control group and three experimental groups (NaF was given orally at the dose of 0, 12, 24 and 48 mg/kg body weight for 42 days, respectively). The results showed that NaF in excess of 12 mg/kg induced the renal histopathological lesions, and inflammatory responses via the activation of nuclear factor-kappa B (NF-κB) signaling pathway and the reduction of anti-inflammatory cytokines expression. The activation of NF-κB signaling pathway was characterized by increasing the nitric oxide (NO) and prostaglandin E2 (PGE2) contents, inducible nitric oxide synthase (iNOS) activities and mRNA expression levels, and the mRNA and protein expression levels of cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) and interleukin-8 (IL-8) in three NaF-treated groups. Concurrently, the mRNA and protein expression levels of the anti-inflammatory cytokines including interleukin-4 (IL-4) and interleukin-10 (IL-10) were decreased in three experimental groups when compared with those in the control group.

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“…Our findings have recently shown that sodium fluoride (NaF) inhibits cell proliferation and induces cell apoptosis in cultured splenic lymphocytes from mice [22, 23]. It has been reported that high fluorine may affect the inflammation autoregulatory processes and the function of immune system [24]. In our previous studies, high fluorine has been found to affect immune organs including thymus [25], spleen [26–28], bursa of Fabricius [29] and cecal tonsil [30–33] by reducing the lymphocyte population and cytokine secretion, inhibiting organ growth, and causing lesions, oxidative stress and apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Sodium fluoride (NaF) causes toxic effects on splenic development in mice

et al. 2016

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At present, very limited studies focus on the toxic effect of sodium fluoride (NaF) on splenic development of human and animals in vivo. This study was firstly designed to evaluate the toxic effects of NaF on the splenic development of mice in vivo by observing histopathological lesions, changes of splenic growth index (GI), T and B cells, immunoglobulin A (IgA), immunoglobulin G (IgG) and immunoglobulin M (IgM) contents, cytokine protein expression levels, and cell cycle and cyclins/cdks protein expression levels using the methods of pathology, flow cytometry (FCM), western blot (WB), and enzyme-linked immunosorbent assay (ELISA). A total of 240 ICR mice were equally allocated into four groups with intragastric administration of distilled water in the control group and 12, 24, 48 mg/kg NaF solution in the experimental groups for 42 days. The results showed that NaF in 12 mg/kg and over caused the toxic effects on splenic development, which was characterized by reducing growth index and lymphocytes in the white and red pulp histopathologically, increasing cell percentages of the G0/G1 phase and decreasing cell percentages of the S phase, and reducing T cells and B cells as well as IgA, IgG, and IgM contents when compared with those in the control group. Concurrently, cytokines including interleukin-2 (IL-2), transforming growth factor beta (TGF-β), tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ) and cyclin (E/D and CDK2/4) protein expression levels were markedly decreased (P < 0.05 or P < 0.01), and interleukin-10 (IL-10) protein expression levels were significantly increased (P < 0.05 and P < 0.01) in the three NaF-treated groups. Toxic effects finally impaired the splenic cellular immunity and humoral immunity due to the reduction of T and B cell population and activity. Cell cycle arrest is the molecular basis of NaF-caused toxic effects on the splenic development.

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Effects of sodium fluoride on immune response in murine macrophages

Cited by 36 publications

References 34 publications

Sodium Fluoride under Dose Range of 2.4–24 μM, a Promising Osteoimmunomodulatory Agent for Vascularized Bone Formation

Sodium Fluoride under Dose Range of 2.4–24 μM, a Promising Osteoimmunomodulatory Agent for Vascularized Bone Formation

Sodium fluoride induces renal inflammatory responses by activating NF-κB signaling pathway and reducing anti-inflammatory cytokine expression in mice

Sodium fluoride (NaF) causes toxic effects on splenic development in mice

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