Effects of sodium butyrate on human chronic myelogenous leukaemia cell line K562

The Histone Deacetylase Inhibitor MS-275 Interacts Synergistically with Fludarabine to Induce Apoptosis in Human Leukemia Cells

“…Recently, considerable attention has focused on HDAC inhibitors as anticancer agents, particularly in hematopoietic malignancies, in view of their ability to induce either differentiation or apoptosis in leukemic cells (15,17). Although it is presumed that such actions result from deacetylation of histones and transcriptional activation of genes involved in these processes, the specific downstream events responsible for induction of cell death or maturation remain to be fully elucidated.…”

“…14). In fact, several HDAC inhibitors (e.g., SAHA, butyrate) have been shown to induce maturation in various human leukemia cell lines (15,16). However, under other circumstances, HDAC inhibitors induce apoptosis rather than maturation, particularly in human leukemia cells (17,18).…”

Histone deacetylase inhibitor enhances the anti-leukemic activity of an established nucleoside analogue

“…Agents like phenylbutyrate and phenylacetate have been shown to potentiate the lethal effects of several conventional anti-leukemic agents. 8,9 It has been previously reported and is evident from this study that HDAC inhibitors may promote free radical formation, interfere with cytoprotective signaling pathways, activate stress-related cascades, reduce expression of some anti-apoptotic proteins and enhances generation of ceramide, one or more of which may be responsible for such synergy with conventional cytotoxic agents.…”

Induction of Differentiation in K562 Cell Line by Nicotinic Acid-Related Compounds

“…Nicotinamide, nicotinamide N-oxide, and several compounds decreased hemoglobin content and -globin expression. K562 cells can be induced to differentiate to a wide variety of mature cells, i.e., PMA induces differentiation to megakaryocyte, 11) and hemin induces erythroid differentiation, 12,13) while 12-O-tetradecanoyl-phorbol-13-acetate (TPA) induces differentiation to monocytes/macrophage, granulocytes, or megakaryocytes.…”

“…11) In contrast, treatment with hemin or sodium butyrate induces differentiation into erythroid lineage. 12,13) K562 cell line provides a useful system for studying human erythroid differentiation because it expresses markers of erythroid lineage, such as hemoglobin. In this study, we investigated the differentiation-inducing ability of nicotinic acid-related compounds in K562 cell line.…”