Effects of sodium butyrate and salinomycin upon intestinal microbiota, mucosal morphology and performance of broiler chickens

Abstract

The effect of dietary sodium butyrate (SB) or salinomycin (SAL) or both additives on performance, small intestinal morphology and microbial ecology of broiler chickens was studied. A growth trial was conducted with 96 Ross 308 female broilers from 1 to 30 days of age. Four treatment groups were fed with a non-supplemented control diet or three experimental diets supplemented with i) 300 mg SB (Adimix 30 coated) per kg, ii) 60 mg SAL (Sacox) per kg or iii) both additives in combination. Feed intake and body-weight gain decreased and gain-to-feed ratio increased due to SAL supplementation, while addition of SB did not affect performance in comparison with the control diet but positively affected feed intake and body-weight gain in comparison with birds fed the SAL-supplemented diet. Villus height in jejunum decreased, while crypt depth increased due to SAL supplementation. Addition of SB increased crypt depth in jejunum. No significant effect of either additive was observed in ileum morphology. Total short-chain organic acids concentration in ileal digesta decreased with SAL supplementation, mainly due to lower lactic acid concentration, but no effects were observed in the caeca. The SAL supplementation was accompanied by a pH increase in ileum and a pH decrease in caecum. No significant effect of SB addition was observed for these parameters. Total bacterial numbers and Lactobacillus [lactic acid bacteria (LAB)] counts in ileal and caecal contents were lower in birds fed with SAL-supplemented diet in comparison with birds fed with control or SB diet. DNA fingerprints revealed SAL supplementation to affect the microbial population by suppressing dominating LAB, potentially L. aviarius. The presented results show that dietary SAL, supplemented alone or in combination with SB, suppressed the microbial activity and altered the microbial community structure mainly in ileum. SAL alone negatively affected feed intake and body-weight gain; however, the effect was ameliorated by SB supplementation.

The Role of Coated Sodium Butyrate on Performance of Broilers Fed High Protein and Reduced Energy Diets

“…These results are in agreement with Smulikowska et al (2009), Zhang et al (2011) and Czerwiński et al (2012. Collectively, these studies suggested that the dietary addition of SB did not have significant effects on WG, FI and FCR of birds grown in hygienic conditions and with high health status (Smulikowska et al, 2009).…”

“…Furthermore, in the current study, SB did not affect ileal and caecal pH values and SCFA contents. Similar findings were reported in literature (Mahdavi and Torki, 2009;Smulikowska et al, 2009;Czerwiński et al, 2012). However, the current study showed that SB at a higher dose (2 g/kg) significantly increased growth rate for the birds fed wheat-based diets.…”

“…Several studies have suggested that dietary SB or its salts had a positive effect on bird performance (Antongiovanni et al, 2007;Taherpour et al, 2009). However, other studies showed no such beneficial effects (Smulikowska et al, 2009;Zhang et al, 2011;Czerwiński et al, 2012).…”

Effect of Organic Acid Salts as an Alternative to Antibiotic Growth Promoters on the Production Performance of Commercial Broiler Chicken

“…The body weight gain obtained in this study with respect to higher level organic acid inclusion agreed with the result of Oruwari et al, (1993) who observed calcium propionate increased the body weight at the dose rate of 1 g/kg and also agreed with Khosravi et al, (2008) who had incorporated the calcium propionate at the dose rate of 2 g/kg. Likewise, in coated sodium butyrate supplemented treatment, results were in agreement with Hu and Guo (2007), Czerwinski et al, 2012, Brozoska et al, (2013 reiterated that sodium butyrate added to the diets broiler enhanced the body weight gain. The organic acid incorporated in the diet might have a positive influence on the digestibility of in-fed nutrients, improves the gut health and that could be the reason for higher body weight gain in the groups supplemented with OAs and the same can be confirmed with the dose dependant improvement noticed in the body weight gain in those groups.…”

The nisin improves broiler chicken growth performance and interacts with salinomycin in terms of gastrointestinal tract microbiota composition

“…The results confirmed that microbiota modulation through ionophore coccidiostats is based on the suppression of Gram-positive pathogens such as Clostridium perfringens (Bjerrum et al, 2005), which causes necrotic enteritis in poultry, and by limiting proliferation of Bacteroides, Clostridiacae, Enterobacteriacae and Lactobacillus spp./Enterococcus spp. which can compete with the host-bird for nutrients uptake as well as impair fat absorption through bile acid deconjugation (Masuda, 1981;Klaver and van der Meer, 1993;Czerwiński et al, 2012).…”