Effects Of Sodium Aurothiomalate On Hyaluronic Acid Synthesis In Normal And Rheumatoid Synovial Fibroblast Cultures

Vuorio, Eero; Takala, I; Pulkki, Kari; Einola, Sakari

doi:10.3109/03009747909114451

Cited by 10 publications

(4 citation statements)

References 9 publications

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“…When added to red cell lysates, a specific binding of gold to cysteine moiety of GSH along with release of thiomalate has been observed. Vuorio et al [16] studied the effects of aurothiomalate on the quantity and quality of HA synthesized by normal and RA fibroblast cultures. They observed that the molecular size of the biopolymer fragments produced by RA fibroblasts were shifted towards normal HA molar-mass values.…”

mentioning

confidence: 99%

Aurothiomalate as Preventive and Chain‐Breaking Antioxidant in Radical Degradation of High‐Molar‐Mass Hyaluronan

Valachová

Vargová

Rapta

et al. 2011

Chemistry & Biodiversity

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The potential anti- or pro-oxidative effects of a disease-modifying antirheumatic drug, aurothiomalate, to protect high-molar-mass hyaluronan against radical degradation were investigated along with L-glutathione - tested in similar functions. Hyaluronan degradation was induced by the oxidative system Cu(II) plus ascorbate known as the Weissberger's oxidative system. The time- and dose-dependent changes of the dynamic viscosity of the hyaluronan solutions were studied by the method of rotational viscometry. Additionally, the antioxidative activity of aurothiomalate expressed as a radical-scavenging capacity based on a decolorization 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay was inspected. At the higher concentrations tested, L-glutathione showed excellent scavenging of (.) OH and peroxyl-type radicals, however, at the lowest concentration applied, its pro-oxidative effect was revealed. The effects of aurothiomalate on hyaluronan degradation were similar to that of L-glutathione, however, at the lowest concentration tested, no significant pro-oxidant effect was observed.

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confidence: 99%

Aurothiomalate as Preventive and Chain‐Breaking Antioxidant in Radical Degradation of High‐Molar‐Mass Hyaluronan

Valachová

Vargová

Rapta

et al. 2011

Chemistry & Biodiversity

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“…RA-FLS and CIA-FLS cultures exhibited populations of fusiform FLSs with cytoplasmic expansions forming branches. Both cell types were anchored to the substrate with numerous cytoplasmic projections at the lamellipodia ends, and the protein structure of the actin cytoskeleton protruded at the mobile end of the cell [23]. Lamellipodia propelled entire cell structures through the substrate (Fig.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Morphofunctional analysis of fibroblast-like synoviocytes in human rheumatoid arthritis and mouse collagen-induced arthritis

et al. 2023

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Background Fibroblast-like synoviocytes (FLS) play a prominent role in rheumatoid synovitis and degradation of the extracellular matrix through the production of inflammatory cytokines and metalloproteinases (MMPs). Since animal models are frequently used for elucidating the disease mechanism and therapeutic development, it is relevant to study the ultrastructural characteristics and functional responses in human and mouse FLS. The objective of the study was to analyze ultrastructural characteristics, Interleukin-6 (IL-6) and Metalloproteinase-3 (MMP-3) production and the activation of intracellular pathways in Fibroblast like synoviocytes (FLS) cultures obtained from patients with rheumatoid arthritis (RA) and from mice with collagen-induced arthritis (CIA). Methods FLSs were obtained from RA patients (RA-FLSs) (n = 8) and mice with CIA (CIA-FLSs) (n = 4). Morphology was assessed by transmission and scanning electron microscopy. IL-6 and MMP-3 production was measured by ELISA, and activation of intracellular signaling pathways (NF-κB and MAPK: p-ERK1/2, p-P38 and p-JNK) was measured by Western blotting in cultures of RA-FLSs and CIA-FLSs stimulated with tumor necrosis factor-alpha (TNF-α) and IL-1β. Results RA-FLS and CIA-FLS cultures exhibited rich cytoplasm, rough endoplasmic reticula and prominent and well-developed Golgi complexes. Transmission electron microscopy demonstrated the presence of lamellar bodies, which are cytoplasmic structures related to surfactant production, in FLSs from both sources. Increased levels of pinocytosis and numbers of pinocytotic vesicles were observed in RA-FLSs (p < 0.05). Basal production of MMP-3 and IL-6 was present in RA-FLSs and CIA-FLSs. Regarding the production of MMP-3 and IL-6 and the activation of signaling pathways, the present study demonstrated a lower response to IL-1β by CIA-FLSs than by RA-FLSs. Conclusion This study provides a comprehensive understanding of the biology of RA-FLS and CIA-FLS. The differences and similarities in ultrastructural morphology and important inflammatory cytokines shown, contribute to future in vitro studies using RA-FLS and CIA-FLS, in addition, they indicate that the adoption of CIA-FLS for studies should take careful and be well designed, since they do not completely resemble human diseases.

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“…Stained cells were examined and photographed using epifluorescence illumination at 485 nm (fluorescein excitation) on a Leitz Dialux 10 photomicroscope. Table 1 have been studied previously for synthesis of hyaluronic acid and other connective tissue macromolecules (43, 17). Most of these cell lines were started from synovial tissue obtained at surgery.…”

Section: Methodsmentioning

confidence: 99%

Arthritis‐associated changes in flow cytometric characteristics of cultured synovial fibroblasts

Eerola

Pulkki

Pelliniemi

et al. 1988

Arthritis & Rheumatism

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Synovial fibroblasts cultured from patients with rheumatoid or reactive arthritis and from controls were studied by flow cytometry, spectroscopy, and electron microscopy. Analysis of 29 cell lines revealed consistent differences between arthritic and normal fibroblasts. Cells cultured from inflamed synovial tissue exhibited higher autofluorescence than did control fibroblasts, and displayed exceptional light scatter properties in flow cytometry, indicating changes in cytoplasmic structures. Electron microscopic examination of the fibroblasts from arthritic synovial tissue revealed large numbers of round, swollen, laminated, mitochondrion-like bodies, which were not observed in the control fibroblasts. The changes observed by flow cytometry (light scatter and autofluorescence) coincided with the presence of the mitochondrion-like organelles. The strong autofluorescence observed in the arthritic fibroblasts resembled the fluorescence spectrum of mitochondrial flavoproteins. These data suggest that persistent metabolic and structural changes have occurred in the mitochondria of synovial fibroblasts and inflammatory synovial tissue. The usefulness of flow cytometry in identifying such cells is described.From the

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Effects Of Sodium Aurothiomalate On Hyaluronic Acid Synthesis In Normal And Rheumatoid Synovial Fibroblast Cultures

Cited by 10 publications

References 9 publications

Aurothiomalate as Preventive and Chain‐Breaking Antioxidant in Radical Degradation of High‐Molar‐Mass Hyaluronan

Aurothiomalate as Preventive and Chain‐Breaking Antioxidant in Radical Degradation of High‐Molar‐Mass Hyaluronan

Morphofunctional analysis of fibroblast-like synoviocytes in human rheumatoid arthritis and mouse collagen-induced arthritis

Arthritis‐associated changes in flow cytometric characteristics of cultured synovial fibroblasts

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