Effects of smokeless tobacco on the uterine wall of adult non pregnant female swiss albino rats

Abstract

This study was aimed to evaluated effects of smokeless tobacco on the uterine wall of female rats of Swiss albino species. This was a controlled experimental study with three groups of adult female Swiss albino rats. The study was conducted over a period of 31 days, at Animal House, Husbandry, and Veterinary Sciences at Sindh Agricultural University, Tandojam, and the Department of Anatomy, Isra University, Faculty of Medicine & Allied Medical Sciences, Hyderabad, Sindh, Pakistan. Thirty adult female Swiss albino rats were randomly assigned to three groups: Group A (control, no treatment), Group B (treated with 5% smokeless tobacco), and Group C (treated with 10% smokeless tobacco). Blood samples were collected on the 31st day to analyze Follicle-stimulating hormone (FSH) and Luteinizing hormone (LH) levels in the serum. After euthanization, uteruses were removed and weighed, followed by histological examination using Hematoxylin & Eosin and Mason's trichrome stains. The experimental groups (B and C) exhibited a significant reduction in uterine weight and decreased serum levels of FSH & LH. Histological examination showed cystically dilated submucosal glands and pronounced atrophy in the uteri of the experimental groups. Additionally, the myometrial wall thickness was also decreased. The use of smokeless tobacco negatively affects the uterine wall of female Swiss albino rats, resulting in histological changes and decreased serum levels of FSH and LH.